[1]杨莹,邢光南,盖钧镒.中国野生大豆对斜纹夜蛾的抗生性鉴定及资源遴选[J].大豆科学,2016,35(03):448-454.[doi:10.11861/j.issn.1000-9841.2016.03.0448]
　YANG Ying,XING Guang-nan,GAI Jun-yi.Evaluation of Antibiosis to Common Cutworm ( Spodoptera litura) and Screeningfor Ｒesistance Sources Among Wild Soybeans ( Glycine soja) in China[J].Soybean Science,2016,35(03):448-454.[doi:10.11861/j.issn.1000-9841.2016.03.0448]
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中国野生大豆对斜纹夜蛾的抗生性鉴定及资源遴选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 448-454 栏目: 出版日期: 2016-05-20
Title:
Evaluation of Antibiosis to Common Cutworm ( Spodoptera litura) and Screeningfor Ｒesistance Sources Among Wild Soybeans ( Glycine soja) in China
作者:
杨莹; 邢光南; 盖钧镒
( 南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室( 综合) /作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心，江苏南京210095)
Author(s):
YANG Ying; XING Guang-nan; GAI Jun-yi
Soybean Ｒesearch Institute of Nanjing Agricultural University /National Center for Soybean Improvement /Key Laboratory for Biology and Genetic Improvementof Soybean( General) ，Ministry of Agriculture /National Key Laboratory for Crop Genetic and Germplasm Enhancement /Jiangsu Collaborative InnovationCenter for Modern Crop Production，Nanjing 210095，China
关键词:
斜纹夜蛾;  野生大豆;  隶属函数;  抗虫性鉴定
Keywords:
Common cutworm ( Spodoptera litura) ;  Wild soybean ( Glycine soja) ;  Membership function index;  Evaluation ofinsect resistance
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0448
文献标志码:
A
摘要:
摘要: 试验在前期对栽培大豆进行抗生性鉴定的基础上， 2014 和2015 年在养虫室喂养斜纹夜蛾初孵幼虫，以喂养第6，9和12 天幼虫重的隶属函数值为抗生性指标评价了来自我国各大豆生态区的200 份代表性野生大豆种质资源的抗虫性。两年第6 天结果的联合方差分析表明: 材料间、年份间、材料与年份互作间差异均达极显著水平。2015 年不同喂养期幼虫重的隶属函数值联合方差分析表明: 材料间、喂养期间差异均达极显著水平，材料与喂养期间无明显互作。按标准品种分级法对供试材料进行分级，发现野生大豆抗生性与地理来源有关，长江中下游生态区( III) 及西南－ 中南生态区( IV) 的高抗材料较多，而北方生态区( I) 的高感材料较多。从供试的野生大豆资源中筛选出高抗和高感材料各10 份，高抗材料的抗性高于国际常用的栽培大豆抗源PI227687 和Lamar，可用于后续的野生大豆抗虫鉴定、抗虫机理及抗虫育种等研究。
Abstract:
Based on our previous studies on evaluation of antibiosis to common cutworm ( Spodoptera litura) in cultivated soybeans( Glycine max) ，the antibiosis of wild soybean ( Glycine soja) was also evaluated in 2014 and 2015. A representativesample composed of 200 wild accessions from the four eco-regions in China were tested in lab conditions with the newlyhatched larva of Spodoptera litura reared in insectarium as tester and the membership index value of the 6th，9th and 12thday’s larva weight as indicator. The results of joint ANOVA of the 6th day’s membership index value in 2014 and 2015showed that there existed significant difference among /between accessions，years and accession × year interactions. The resultsof joint ANOVA of different feeding duration in 2015 showed that it existed significant difference among different feeding durationand accessions，while accession × feeding-duration interactions did not show significant difference. The accessions weregrouped into highly resistant，resistant，medium resistant，susceptible and highly susceptible grades using a standard checkgrade system. A significant relationship was detected between eco-region and resistance grade，more resistant wild accessionsexisted in Middle and Lower Yangtse Ｒiver Eco-region( III) and Southwest-Central South Eco-region( IV) ，while more susceptiblewild accessions existed in North Eco-region( I) . Ten highly resistant and ten highly susceptible wild soybean accessionswere screened out as checks and resistance source materials for further study on the resistance mechanism and resistancebreeding. The ten highly resistant wild soybean accessions performed higher resistance than the highly resistant cultivated soybeanPI227687，Lamar and other resistance sources using the same resistance evaluation system.

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备注/Memo

基金项目: 国家重点基础研究发展计划“973 计划”( 2011CB1093) ; 国家自然科学基金( 31571694) ; 农业部公益性行业专项( 201203026-4) ; 教育部111 项目( B08025) ; 教育部长江学者和创新团队( PCSＲT13073) ; 中央高校基本科研业务费项目( KYZ201202-8，KYZ201504) ; 国家现代农业产业技术体系( CAＲS-04) ; 江苏省优势学科建设工程专项; 江苏省JCIC-MCP 项目。

第一作者简介: 杨莹( 1991-) ，女，硕士，主要从事大豆抗虫育种研究。E-mail: 2013101157@ njau. edu. cn。
通讯作者: 盖钧镒( 1936-) ，男，教授，主要从事大豆种质资源与遗传育种研究。E-mail: sri@ njau. edu. cn;邢光南( 1980-) ，男，博士，副教授，主要从事大豆抗虫育种研究。E-mail: xinggn@ njau. edu. cn。

更新日期/Last Update: 2016-06-29