DONG Ya-nan,WEI Lai,DING Jun-jie,et al.Excavation the Resistance Locus to Cercospora sojina Hara Race 12 in Soybeanby Association Analysis[J].Soybean Science,2016,35(03):402-406.[doi:10.11861/j.issn.1000-9841.2016.03.0402]
应用关联分析挖掘大豆对灰斑病12 号生理小种的抗性位点
- Title:
- Excavation the Resistance Locus to Cercospora sojina Hara Race 12 in Soybeanby Association Analysis
- Keywords:
- Cercospora sojina; Resistance locus; Association analysis
- 分类号:
- S565. 1
- 文献标志码:
- A
- 摘要:
- 利用126 份SSR 标记对320 份对大豆灰斑病12 号生理小种具有不同抗性的大豆材料进行基因组扫描、群体结构和连锁不平衡分析,并用STRUCTURE 软件、TASSEL 软件中的GLM 分析方法对大豆抗性进行关联分析。结果表明: 320 份大豆品种中有23 个表现高抗, 53 个表现抗病, 178 个表现中抗, 56 个感病, 10 个高感。找到与大豆抗灰斑病12 号生理小种的相关位点共7 个: H 连锁群上的Satt052、F 连锁群上的Satt335、C2 连锁群上的Satt557、A1 连锁群上的Sat_368、D1a 连锁群上的Sat_346、O 连锁群上的Satt243、J 连锁群上的Sat_151。
- Abstract:
- Genomic scanning,population structure and linkage disequilibrium analysis were carried out on 320 physiologicalraces of soybean with different resistance to gray leaf spot disease 12 by using 126 SSR markers. Soybean resistance associationanalysis to Cercospora sojina Hara race 12 were run with software STRUCTURE and GLM procedure from software TASSEL.Research results showed that there were 23 soybean varieties showed high resistance,53 showed resistance to disease,178 were resistant,56 were susceptible,and 10 were high susceptible. Seven resistance locus to Cercospora sojina Hara race12 were found ineluding Satt052 on H linkage group,Satt335 on F linkage group,Satt557 on C2 linkage group,Sat_368 onA1 linkage group,Sat_346 on D1a linkage group. Satt243 on O linkage group,Sat_151 on J linkage group.
参考文献/References:
[1] 张文慧,杜吉到,陈庆山,等. 抗大豆灰斑病育种研究进展[J]. 黑龙江八一农垦大学学报,2006,18( 1) : 22-26. ( ZhangW H,Du J D,Chen Q S,et al. Advances on breeding for frogeyeleaf spot resistance of soybean[J]. Journal of Heilongjiang AugustFirst Land Reclamation University,2006,18( 1) : 22-26. )
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备注/Memo
基金项目: 黑龙江省农业科技创新工程( 2014ZD011 ) ; 哈尔滨市应用技术与开发项目( 2013RFQYJ016 ) ; 国家现代农业产业技术体系( CARS-04-PS05) 。