[1]鲁建聪,许艳丽,宋洁. 土壤扰动对寄生真菌代谢物温室防治大豆胞囊线虫的影响[J].大豆科学,2016,35(01):106-111.[doi:10.11861/j.issn.1000-9841.2016.01.0106]
　LU Jian-cong,XU Yan-li,SONG Jie.Effect of Soil Disturbance to the Biological Control of Fermentation Filtrate of Parasitic Fungi on Soybean Cyst Nematode in Glasshouse[J].Soybean Science,2016,35(01):106-111.[doi:10.11861/j.issn.1000-9841.2016.01.0106]
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土壤扰动对寄生真菌代谢物温室防治大豆胞囊线虫的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 106-111 栏目: 出版日期: 2016-01-20
Title:
Effect of Soil Disturbance to the Biological Control of Fermentation Filtrate of Parasitic Fungi on Soybean Cyst Nematode in Glasshouse
作者:
鲁建聪1; 2; 许艳丽2; 宋洁1; 2
1.中国科学院 东北地理与农业生态研究所/黑土区农业生态院重点实验室，黑龙江 哈尔滨 150081；
?2.东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
LU Jian-cong1; 2;  XU Yan-li2;  SONG Jie1; 2
1.Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences/Key Laboratory of Mollisols Agroecology, Harbin 150081, China;?
2.College of Agriculture, Northeast Agricultural University, Harbin 150040, China
关键词:
扰动; 大豆胞囊线虫; 寄生真菌; 代谢物
Keywords:
Disturbance;  Soybean cyst nematode;  Parasitic fungi;  Fermentation filtrate
DOI:
10.11861/j.issn.1000-9841.2016.01.0106
文献标志码:
A
摘要:
大豆胞囊线虫是影响大豆产量的主要限制性病原。大豆胞囊线虫的自然抑制性是存在的，并且日益受到关注，然而在胞囊线虫生态抑制方面的研究却很少。研究在温室条件下接种真菌后以扰动（土壤过0.5 cm孔筛）为土壤微环境并与无干扰对比，用厚垣轮枝菌（Pochonia chlamydosporium)、镰孢菌（Fusarium spp.）和淡紫拟青霉（Paecilomyces lilacinus）的混合代谢物分别对大豆胞囊线虫进行处理，探讨其对大豆胞囊线虫温室盆栽防效的影响。在接种二龄幼虫（J2）35 d后，对大豆胞囊线虫密度调查结果表明：土壤扰动条件下，接种混合代谢物与不接种混合代谢物相比，雌虫数量降低20.0%~35.9%，胞囊数量降低18.3%~35.0%，卵量降低21.5%~38.5%，根内J2数量降低14.6%~35.3%，其中以4号混合代谢物扰动对大豆胞囊线虫密度降低作用显著；土壤不扰动下，接种混合代谢物与不接种混合代谢物相比，雌虫密度降低19.5%~32.7%，胞囊密度降低19.5%~35.2%，卵量降低21.1%~37.8%，J2数量降低8.0%~27.6%，仅雌虫和根内J2数量与不接种混合代谢物对照差异显著，菌株之间差异不显著。对大豆植株生长发育调查结果表明：扰动可能会促进植株生长、植株鲜重增加，但与不扰动差异不显著。试验证明：扰动对盆栽大豆胞囊线虫雌虫、胞囊、卵和J2的密度有一定的影响，扰动有增强寄生真菌代谢物对胞囊线虫温室防效效果的趋势，对降低大豆胞囊线虫密度起到积极作用。真菌对大豆胞囊线虫生长发育具有抑制作用，土壤扰动干扰和真菌代谢物的配合应用会降低土壤中胞囊线虫的密度，并影响真菌在土壤中的生防效果。
Abstract:
Soybean cyst nematode (SCN), Heterodera glycines, remains a major yield-limiting pathogen of soybean. Natural suppression of SCN exists and becomes increasingly attractive. However, ecological mechanism leading to the suppressive state of cyst nematode is rarely studied.The glasshouse experiments were performed to determine the effects of soil disturbance and fermentation filtrate of parasitic fungal on soybean cyst nematode.Soil disturbance was simulated by soil passing through a sieve(aperture 0.5 cm) and compared with no-disturbance (non-sieve) treatment. Different fermentation filtrate of parasitic fungi were mixed by applying.Paecilomyces lilacinus, Fusarium spp. and Pochonia chlamydosporium.Density of SCN were investigated 35 days after soybeans planted in each pot- It showed that the SCN female, density of cyst, egg and J2 were significantly reduced with 20.0%-35.9%, 18.3%-35.0%, 21.5%-38.5% and 14.6%-35.3% respectively in the treatment of soil disturbance with mixed fermentation filtrate compared with soil disturbance without mixed fermentation, and the disturbance of the 4th mixed fermentation filtrate on soybean cyst nematode density was the most significantly. Without soil disturbance, applying mixed fermentation filtrate could reduce the SCN female, density of cyst, egg and J2 by 19.5%-32.7%, 19.5%-35.2%, 21.1%-37.8% and 8.0%-27.6% respectively.Compared with control treatments, there were no significant difference in the population of SCN female, when J2 treated with different mixed fermentation filtrate. This study also showed that soil disturbance could stimulate the growth of soybean, and increase the fresh weight of soybean plants, but there were no significant difference between those without disturbance.These results indicated that mixed fermentation filtrate could inhibit the development of the SCN female, density of cyst, egg and J2.Soil disturbance could promote preventive effectiveness of fungus metabolism in glasshouse and play an active role on reducing the population density of SCN. Fungi inhibit the growth and development of SCN, coordination application of soil disturbance interference and fungal metabolites might induce the population density of SCN and affect its biological control efficiency in soil.

参考文献/References:

［1］刘维志. 植物病原线虫学 ［M］.北京: 中国农业出版社, 2000: 28l-294. (Liu W Z. Plant pathogenic nematodes ［M］. Beijing: China Agriculture Press, 2000: 28l-294.)

［2］Hartwig E E. Breeding productive soybean cultivars resistant to the soybean cyst nematode for the southern United States［J］. Plant Disease, 1981, 65(4): 303-305.
［3］许艳丽. 土壤环境对大豆胞囊线虫卵孵化影响及线虫分子诊断研究 ［D］. 哈尔滨: 东北农业大学, 2004.(Xu Y L.The effects of soil environment on soybean cyst nematode egg hatch and nematode molecular diagnosis ［D］.Harbin：Northeast Agricultural University, 2004.)
［4］Chen S Y. Suppression of Heterodera glycines in soils from fields with long-term soybean monoculture ［J］. Biocontrol Science and Technology, 2007, 17(2): 125-134
［5］孙玉秋, 许艳丽, 李春杰, 等.作物轮作系统对土壤中大豆胞囊线虫胞囊量和单胞囊卵量的影响 ［J］.农业系统科学与综合研究, 2011, 27(2): 298-251. (Sun Y Q, Xu Y L, Li C J, et al. Influence of cropping rotation systems on the volume of cysts and eggs in a single cyst of soybean cyst nematode ［J］. System Sciences and Comprehensive Studies Agriculture, 2011, 27(2): 298-251.)
［6］靳学慧, 辛惠普, 郑雯, 等. 长期轮作和连作对土壤中大豆胞囊线虫数量的影响 ［J］. 中国油料作报, 2006, 28(2): 189-193. (Jin X H, Xin H P, Zheng W, et al. The influence of soil on the long term rotation and continuous collision on soybean cyst nematode ［J］.Chinese Journal of Oil Crop Sciences, 2006, 28(2): 189-193.)
［7］陈立杰, 段玉玺, 范圣长, 等. 大豆胞囊线虫病的生防因子研究进展 ［J］. 西北农林科技大学学报(自然科学版), 2005, 33(增):190-194.(Chen L J, Duan Y X, Fan S C, et al.Advances in antagonists of soybean cyst nematode ［J］. Journal of Northwest A ＆ F University (Natural Science Edition), 2005, 33(S): 190-194.)
［8］卢明科, 潘沧桑, 李舟. 厚垣轮枝孢菌(Verticillium chlamydosporium)防治植物线虫研究进展 ［J］. 西北农林科技大学学报（自然科学版）, 2004, 32(4): 103-105.(Lu M K, Pan C S, Li Z.Advances of studies of Verticillium chlamydosporium as a biological control agent for plant parasitic nematodes ［J］.Journal of Northwest A & F University (Natural Science Edition), 2004, 32(4): 103-105.)
［9］赵晓晖, 许艳丽. 镰刀菌发酵液对大豆胞囊线虫的抑制作用 ［J］.大豆科学, 2011, 30(3): 468-470. (Zhao X H, Xu Y L. Inhibition of Fusarium.spp fermented filtrates on soybean cyst nematode ［J］ Soybean Science, 2011, 30(3): 468-470.)
［10］Sun M H, Gao L, Shi Y X, et al.Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in china and their biocontrol potential ［J］.Journal of Invertebrate Pathology, 2006, 93: 22-28.
［11］孙玉秋, 许艳丽, 李春杰, 等. 东北地区大豆胞囊线虫卵定殖真菌的多样性研究 ［J］.华北农学报, 2011, 26(2)：233-238. (Sun Y Q, Xu Y L, Li C J, et al. Mycofloras in eggs of soybean cyst nematode in northeast China ［J］. Acta Agriculture Boreall-Sinica, 2011, 26(2)：233-238.)
［12］陈立杰, 刘小杰, 段玉玺, 等. 轮作和连作田大豆胞囊线虫胞囊上真菌定殖动态 ［J］ 大豆科学, 2009, 28(2)：266-270. (Chen L J, Liu X J, Duan Y X, et al. Dynamics of fungial colonization on cyst in rotation and continuous cropping of soybean ［J］.Soybean Science, 2009, 28(2)：266-270.)
［13］赵晓晖, 许艳丽. 厚垣轮枝菌发酵液对大豆胞囊线虫的抑制作用 ［J］. 大豆科技，2011(2):19-25. (Zhao X H, Xu Y L. Inhibition of Verticillium chlamydosporium fermented filtrates on soybean cyst nematode ［J］.Soybean Science and Technology, 2011(2)：19-25.)
［14］赵晓辉. 大豆胞囊线虫抑制性土壤中寄生真菌及其作用研究 ［D］.哈尔滨：东北林业大学, 2011.(Zhao X H.Suppressive soil of soybean cyst nematode and parasitic fungi ［D］. Harbin: Northeast Forestry University, 2011.)
［15］葛红莲, 郭坚华, 祁红英, 等. 复合菌剂AR99防治辣椒青枯病 ［J］. 植物病理学报, 2004, 34(2)：162-165.(Ge H L, Guo J H, Qi H Y, et al. Biological control of Capsieum baeterial wilt by compound baeterial mixture AR99 ［J］. ACTA Phytopathologica Sinica, 2004, 34(2)：162-165.)
［16］王波, 李红梅, 王碧, 等. 淡紫拟青霉与放线菌代谢物复配对南方根结线虫的防治 ［J］. 南京农业大学学报, 2009, 32(1)：55-60.(Wang B, Li H M, Wang B, et al. Biological control of Meloidogyne incognita by combination of Paecilomyces lilacinus and Actinomycetes spp. ［J］. Journal of Nanjing Agricultural University, 2009, 32(1)：55-60.)
［17］钱洪利. 明尼苏达被毛袍对大豆胞囊线虫作用的研究 ［D］. 哈尔滨：东北农业大学, 2009.(Qian H L.The effect of Hirsutella minnestensisisa on soybean cyst nematode ［D］.Harbin: Northeast Agricultural University, 2009.)
［18］Bao Y, Neher D A, Chen S. Effect of soil disturbance and biocides on nematode communities and extracellular enzyme activity in soybean cyst nematode suppressive soil ［J］. Nematology, 2011, 13(6)：687-699.
［19］Jenkins W R. A rapid centrifugal flotation technique for separating nematodes from soil ［J］. Plant Disease, Report, 1964, 48：692
［20］范圣长, 段玉玺, 陈立杰.大豆胞囊线虫胞囊内寄生真菌研究 ［J］.大豆科学, 2004, 23(1)：71-74. (Fan S C, Duan Y X, Chen L J. The reserach on the cyst entoparasitic fungi of soybean cyst nematode ［J］.Soybean Science, 2004, 23(1)：71-74.)

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备注/Memo

基金项目：国家自然科学基金（39071900）。

第一作者简介：鲁建聪（1989-），女，硕士，主要从事大豆胞囊线虫防治研究。E-mail:cong1048063166@163.com。
通讯作者：许艳丽（1958-），女，研究员，博导，主要从事植物线虫病害、作物病虫害生物生态控制和土壤微生态研究。E-mail:xyll@iga.ac.cn。

更新日期/Last Update: 2016-02-05