[1]柏宇,关大伟,李力,等.耐高氮优良大豆根瘤菌株的筛选与鉴定[J].大豆科学,2014,33(06):861-865.[doi:10.11861/j.issn.1000-9841.2014.06.0861]
　BAI Yu,GUAN Da-wei,LI Li,et al.Screening and Characterization of Superior Nitrogen-Tolerance Soybean Rhizobia[J].Soybean Science,2014,33(06):861-865.[doi:10.11861/j.issn.1000-9841.2014.06.0861]
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耐高氮优良大豆根瘤菌株的筛选与鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 861-865 栏目: 出版日期: 2014-12-25
Title:
Screening and Characterization of Superior Nitrogen-Tolerance Soybean Rhizobia
文章编号:
1000-9841.2014.06.0861
作者:
柏宇; 关大伟; 李力; 姜昕; 马鸣超; 李俊
中国农业科学院 农业资源与农业区划研究所/农业部微生物产品质量安全风险评估实验室，北京 100081
Author(s):
BAI Yu; GUAN Da-wei; LI Li; JIANG Xin; MA Ming-chao; LI Jun
Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Microbial Products,Ministry of Agriculture,Beijing 10081,China
关键词:
筛选; 大豆根瘤菌; 耐氮; 优良菌株
Keywords:
Screening; Soybean rhizobia; Nitrogen-tolerance; Superior strain
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0861
文献标志码:
A
摘要:
采用高氮平板与蛭石盆栽相结合的方法，以黑龙江省农业科学院1979年设立的黑土长期定位试验7种不同施肥处理中分离到的254株大豆根瘤菌为材料，筛选耐高氮的优良大豆根瘤菌。高氮平板结果显示：随着尿素浓度的增加，可生长的菌株数量逐渐减少，其中严重抑制菌株生长的尿素浓度为5 g.L-1，该浓度条件下只有11株菌能够生长，均来自连续施用氮肥的处理。进一步采用模拟高氮环境蛭石盆栽的方法，将能在5 g.L-1尿素条件下生长的11株菌进行复筛，以植株干重、植株全氮量、根瘤干重和数量作为评价指标，获得1株在高氮条件下具有结瘤固氮能力的菌株5841。经16S rDNA序列系统发育分析，初步确定该菌株属于日本慢生大豆根瘤菌（Brandyrhizobium japonicum)。
Abstract:
The superior nitrogen-tolerance rhizobia strains were screened from 254 strains of rhizobia,which were isolated from 7 different fertilization treatments in long-term black soil fertility and fertilizer efficiency experiment station adopting high-concentration nitrogen plate method and vermiculite pot.The black soil fertility and fertilizer efficiency experiment station were constructed by Heilongjiang Academy of Agricultural Sciences in 1979.The results obtained from high-concentration nitrogen plate method indicated that increasing urea concentration had an increased bacteriostatic effect.Urea concentration which could seriously inhibit growth of strains was 5 g.L-1,only 11 strains could grow in this urea concentration,which were all isolated from continuous application of nitrogen fertilizer.In the subsequent high-concentration nitrogen vermiculite pot experiment,the best strain 5841 were selected according to the plant dry weight,number of nodules,dry weight of nodules and total N content of the plant,which had the ability of nodulation nitrogen fixation in high-concentration nitrogen.Based on 16S rDNA sequence phylogenetic analysis,the strain 5841 was identified as Brandyrhizobium japonicum.

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备注/Memo

基金项目：现代农业产业技术体系建设专项(CARS-04)；中央级公益性科研院所基本科研业务费专项资金（2013-2）；国家高技术研究发展计划“863计划”（2013AA102802-04）。

第一作者简介：柏宇（1988-），男，硕士,主要从事生物固氮研究。E-mail:bby1116@163.com。

更新日期/Last Update: 2014-12-29