[1]李易初,张俊华.大豆菌核病菌不同菌丝亲和群的遗传差异性研究[J].大豆科学,2014,33(05):718-723.[doi:10.11861/j.issn.1000-9841.2014.05.0718]
　LI Yi-chu,ZHANG Jun-hua.Genetic Diversity of Sclerotinia Sclerotiorum in Soybean in Different Mycelia Compatibility Groups[J].Soybean Science,2014,33(05):718-723.[doi:10.11861/j.issn.1000-9841.2014.05.0718]
点击复制

大豆菌核病菌不同菌丝亲和群的遗传差异性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 718-723 栏目: 出版日期: 2014-10-25
Title:
Genetic Diversity of Sclerotinia Sclerotiorum in Soybean in Different Mycelia Compatibility Groups
文章编号:
1000-9841.2014.05.0718
作者:
李易初1; 2; 张俊华1
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
2.黑龙江省农业科学院 植物保护研究所，黑龙江 哈尔滨 150086
Author(s):
LI Yi-chu1; 2; ZHANG Jun-hua1
1.Agronomy College，Northeast Agricultural University,Harbin 150030,China;
2.Plant Protection Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China
关键词:
大豆菌核病; 菌丝亲和组群; 形态学; 遗传差异
Keywords:
Soybean Sclerotinia sclerotiorum; Mycelia compatibility group; Morphology; Genetic diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0718
文献标志码:
A
摘要:
为明确大豆菌核病菌不同菌丝亲和群的遗传差异性，经菌丝亲和组群（MCG)方法筛选，挑选12个独自成群有代表性菌株，以常规PDA培养基培养，比较其菌落形态、生长速率和菌核的各项形态学特性，并采用SSR分子标记分析其遗传多态性。结果表明：不同菌丝亲和群菌株之间，形态学差异较大，菌落形状、颜色和菌丝丰度均分为两类；菌核重量和大小等差异性显著，直径变化范围0.13～0.53 cm，菌核粒重变幅0.007～0.340 g；SSR分子标记技术与MCG方法分析核盘菌遗传差异性结果一致，且相似系数越高，一致性越高。可见，黑龙江地区大豆菌核病菌丝亲和群数量多，菌株之间存在多元性和差异性，核盘菌种群内存在较大的异质性，遗传类型复杂。
Abstract:
To identify genetic diversity of isolates of Sclerotinia sclerotiorum- from different mycelia compatibility groups,12 alone representative strains of different MCGs were selected by the Mycelia compatibility groups(MCG)screening methods.The strains were cultivated by PDA medium,and the colony characteristics,growth rate of mycelium and morphological features of sclerotia were compared.Genetic polymorphism was studied by SSR molecular marker.The results showed that the differences were significant in colony characteristics,mycelium growth rate,Sclerotia location on medium,the total number of Sclerotia and Sclerotia weight among the strains from different MCGs.The colony shape,colony color and hyphal fullness degree of strains were divided into two categories.Sclerotium diameter changed from 0.13 to 0.53 cm,Sclerotium grain weight ranged from 0.007 to 0.340 g.The average growth rate of strain was 2.206 cmd-1,the capability sclerotial formation was not affected by mycelium growth rate.The result of SSR molecular marker technology was similar with MCG’s,and the higher of the similarity coefficient,the higher consistence.Therefore,there are scores of mycelia compatibility groups of S.sclerotiorum in soybean in Heilongjiang province,the strains of S.sclerotiorum had polyphyly and diversity.It indicated the presence of heterogeneity and heredity types diversity of S.Sclerotiorum infecting soybean in Heilongjiang province.

参考文献/References:

［1］陈卫民,陈祥,宋红梅,等.新疆大豆菌核病研究初报[J].大豆通报,2003(5):14.(Chen W M,Chen X,Song H M,et al.The preliminary report of soybean Sclerotinia sclerotiorum in Xinjiang region[J].Soybean Bulletin,2003(5):14.)

[2]Bolton M,Thomma B,Nelson B.Sclerotinia sclerotiorum(Lib.)de Bary:biology and molecular traits of a cosmopolitan pathogen[J].Molecular Plant Pathology,2006,7(1):1-6.
[3]赵丹,许艳丽,李春杰.大豆菌核病的识别与综合防治[J].大豆通报,2006(3):15-16.(Zhao D,Xu Y L,Li C J.Identification and integrated managements for soybean Sclerotinia-sclerotiorum[J].Soybean Bulletin,2006(3):15-16.)
[4]董志敏,王曙明,刘玉芝,等.大豆抗菌核病研究进展[J].大豆科学,2008,27(6):1053-1057.(Dong Z M,Wang S M,Liu Y Z,et al.Progress on resistance to Sclerotinia sclerotiorum in soybean[J].Soybean Science,2008,27(6):1053-1057.)
[5]董全中.大豆菌核病的发生规律及综合防治[J].大豆通报，2003(3):56-63.(Dong Q Z.Occurrence of soybean Sclerotinia and integrated control[J].Soybean Bulletin,2003(3):56-63.)
[6]韩立新,刘颖,吴晓艳,等.大豆菌核病流行原因分析及综合治理措施[J].上海农业科技,2006(6):35-41.(Hang L X,Liu Y,Wu X Y,et al.Popular reasons analysis of soybean Sclerotinia and comprehensive control measures[J].Shanghai Agricultural Science and Technology,2006(6):35-41.)
[7]刘宇.大豆菌核病的发生及防治措施[J].黑龙江科技信息,2010,29:253.(Liu Y.Occurrence of soybean Sclerotinia and prevention measures[J].Heilongjiang Scientific and Technological Information,2010,29:253.)
[8]孙君明.不同菌核病(Sclerotinia sclerotiorum)分离物的形态学、致病性和遗传多样性的研究[D].北京：中国农业大学,2005.(Sun J M.Determination of morphology,pathogenicity and genetic diversity of Sclerotinia sclerotiorum(Lib.)de Bary isolates from different origins[D].Beijing:China Agricultural University,2005.)
[9]李佳,沈斌章,韩继祥,等.一种有效提取油菜叶片总DNA的方法[J].华中农业大学学报,1994,13(5):521-523.(Li J,Shen B Z,Han J X,et al.An effective method of extract rapeseed leaf DNA[J].Journal of Huazhong Agricultural University,1994,13(5):521-523.)
[10]王玉杰.向日葵菌核病病原菌遗传多样性及致病力分化的研究[D].呼和浩特:内蒙古农业大学,2011.(Wang Y J.Genetic diversity and pathogenicity variation of Sclerotinia sclerotiorum in Sunflower[D].Hohhot:Inner Mongolia:Inner Mongolia Agricultural University,2011.)
[11] Sebastian R L,Howell E C,King G J,et al.An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping population[J].Theoretical and Applied Genetics,2000,100(1):75-81.
[12]刘东,代丽婷,蒲子婧,等.黄瓜枯萎病菌毒力、营养体亲和性及ISSR分析[J].植物病理学报,2012,42(5):456-465.(Liu D,Dai L T,Pu Z J,et al.Virulence,vegetative compatibility and ISSR analysis of Fusarium oxysporum f.Sp.Cucumarinum[J].Acta Phytopathologica Sinica,2012,42(5):456-465.)
[13]战宇航,宋巍巍,范冬梅,等.黑龙江省不同地区大豆菌核病病原菌分离物的形态学分析[J].大豆科学,2010,29(1):72-76.(Zhan Y H,Song W W,Fan D M,et al.Morphological trait analysis of soybean Sclerotinia sclerotiorum isolates derived from different location of Heilongjiang province[J].Soybean Science,2010,29(1):72-76.)
[14] Akram A,Iqbal S M,Ahmad N,et al.Morphological variability and mycelial compatibility among the isolates of Sclerotinia sclerotiorum associated with stem rot of chickpea[J].Pakistan Journal of Botany,2008,40(6):2663-2668.
[15]张吉清,张学雷,肖炎农,等.黑龙江省大豆菌核病菌生长特性及菌丝体亲和型测定[J].中国油料作物学报,2013,35(3):307-312.(Zhang J Q,Zhang X L,Xiao Y N,et al.Biological characteristics and mycelial compatibility of Sclerotinia sclerotiorum from soybean in Heilongjiang province[J].Chinese Journal of Oil Crops Sciences,2013,35(3):307-312.)

相似文献/References:

[1]顾 鑫.气象因素对三江平原大豆菌核病的影响及预测模型的创建[J].大豆科学,2013,32(05):680.[doi:10.11861/j.issn.1000-9841.2013.05.0680]
　GU Xin.Effects of Meteorological Factors on Soybean Sclerotinia sclerotiorum in Sanjiang Plain and Related Prediction Model Establishment[J].Soybean Science,2013,32(05):680.[doi:10.11861/j.issn.1000-9841.2013.05.0680]
[2]丁俊杰.通径分析在大豆菌核病流行中的应用[J].大豆科学,2012,31(02):327.[doi:10.3969/j.issn.1000-9841.2012.02.036]
　DING Jun-jie.Application of Path Analysis in Soybean Sclerotinia Sclerotiorum Prevalence[J].Soybean Science,2012,31(05):327.[doi:10.3969/j.issn.1000-9841.2012.02.036]
[3]顾 鑫.不同施肥条件下大豆菌核病病情指数与影响因素的通径分析[J].大豆科学,2014,33(04):607.[doi:10.11861/j.issn.1000-9841.2014.04.0607]
　GU Xin.Path Analysis of the Sclerotinia Sclerotiorum Index of Soybean with Influencing Factors under Different Fertilization Conditions[J].Soybean Science,2014,33(05):607.[doi:10.11861/j.issn.1000-9841.2014.04.0607]
[4]张毅瑞,滕卫丽,李文滨.国内外大豆菌核病鉴定方法研究现状[J].大豆科学,2010,29(01):161.[doi:10.11861/j.issn.1000-9841.2010.01.0161]
　ZHANG Yi-rui,TENG Wei-li,LI Wen-bin.Comparison on the Methods of Evaluating Soybean Sclerotinia sclerotiorum in China and Foreign Countries[J].Soybean Science,2010,29(05):161.[doi:10.11861/j.issn.1000-9841.2010.01.0161]
[5]董志敏,王曙明,刘玉芝,等.大豆抗菌核病研究进展[J].大豆科学,2008,27(06):1053.[doi:10.11861/j.issn.1000-9841.2008.06.1053]
　DONG Zhi-min,WANG Shu-ming,LIU Yu-zhi,et al.Progress on Resistance to Sclerotinia Sclerotiorum in Soybean[J].Soybean Science,2008,27(05):1053.[doi:10.11861/j.issn.1000-9841.2008.06.1053]
[6]孙明明,韩英鹏,陈浩,等.大豆菌核病鉴定方法比较及分析[J].大豆科学,2007,26(05):728.[doi:10.3969/j.issn.1000-9841.2007.05.018]
　SUN Ming-ming,HAN Ying-peng,CHEN Hao,et al.COMPARISONS AND ANALYSES ON THE METHODS OF EVALUATING TOLERANCE TO SOYBEAN WHITE MOULD[J].Soybean Science,2007,26(05):728.[doi:10.3969/j.issn.1000-9841.2007.05.018]
[7]董利东,王金生,吴俊江,等.野生大豆种质资源对大豆菌核病抗性评价[J].大豆科学,2014,33(06):900.[doi:10.11861/j.issn.1000-9841.2014.06.0900]
　DONG Li-dong,WANG Jin-sheng,WU Jun-jiang,et al.Identification the Resistance of Wild Soybean Germplasm to Sclerotinia sclerotiorum[J].Soybean Science,2014,33(05):900.[doi:10.11861/j.issn.1000-9841.2014.06.0900]
[8]孙明明,韩英鹏,赵雪,等.大豆菌核病鉴定方法研究进展[J].大豆科学,2017,36(03):470.[doi:10.11861/j.issn.1000-9841.2017.03.0470]
　SUN Ming-ming,HAN Ying-peng,ZHAO Xue,et al.Research Development of Identification Methods in Soybean White Mold[J].Soybean Science,2017,36(05):470.[doi:10.11861/j.issn.1000-9841.2017.03.0470]

备注/Memo

基金项目：国家公益性行业(农业)科研专项(201103016)。

第一作者简介：李易初（1986-），硕士，助理研究员，主要从事植物病理学研究。E-mail:liyichu0724@163.com
。通讯作者：张俊华（1973-），男，教授，博导，主要从事病原物与寄主植物分子互作、植物病害及防治研究。E-mail:podozjh@163.com。

更新日期/Last Update: 2014-12-27