[1]段玉玺,李海燕,陈立杰,等.大豆不同品种根内类黄酮提取物对大豆胞囊线虫的抑制作用[J].大豆科学,2014,33(05):724-727.[doi:10.11861/j.issn.1000-9841.2014.05.0724]
　DUAN Yu-xi,LI Hai-yan,CHEN Li-jie,et al.Inhibitory Effects of Flavonoids Extracted from Different Soybean Root on Heterodera glycines Ichinohe[J].Soybean Science,2014,33(05):724-727.[doi:10.11861/j.issn.1000-9841.2014.05.0724]
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大豆不同品种根内类黄酮提取物对大豆胞囊线虫的抑制作用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 724-727 栏目: 出版日期: 2014-10-25
Title:
Inhibitory Effects of Flavonoids Extracted from Different Soybean Root on Heterodera glycines Ichinohe
文章编号:
1000-9841.2014.05.0724
作者:
段玉玺1; 李海燕1; 2; 陈立杰1; 陈井生1; 3
1.沈阳农业大学 植物保护学院/北方线虫研究所，辽宁 沈阳 110866；
2.黑龙江八一农垦大学 农学院，黑龙江 大庆 163316；
3.黑龙江省农业科学院 大庆分院，黑龙江 大庆 163313
Author(s):
DUAN Yu-xi1; LI Hai-yan1; 2; CHEN Li-jie1; CHEN Jing-sheng1; 3
1.Nematology Institute of Northern China/Department of Plant Protection，Shenyang Agricultural University，Shenyang 110866,China；
2.Agricultural College，Heilongjiang Bayi Agricultural University,Daqing 163316,China；
3.Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316,China
关键词:
大豆根; 大豆胞囊线虫; 类黄酮提取物; 蛋白质; 糖
Keywords:
Soybean root; Heterodera glycines Ichinohe; Flavonoids extraction; Protein; Glucose
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0724
文献标志码:
A
摘要:
以对大豆胞囊线虫表现不同抗性的五寨黑豆（抗)和合丰35（感)为材料，利用乙醇为有机溶剂，提取大豆根内类黄酮化合物，测定类黄酮提取物对大豆胞囊线虫卵的孵化及J2幼虫的杀线活性。结果表明：大豆不同品种根内类黄酮粗提物对大豆胞囊线虫卵的孵化、J2幼虫均有一定的抑制作用。处理24，48和72 h后，五寨黑豆对卵孵化的抑制率比合丰35分别提高12.76%、9.03%和9.37%。处理48 h，合丰35的校正死亡率比五寨黑豆增加7.0%。抗、感品种根内类黄酮提取物对大豆胞囊线虫J2的生理毒性结果表明：抗、感病品种根内类黄酮提取物均可有效抑制大豆胞囊线虫体内蛋白质和糖的含量。对蛋白质含量的抑制率分别为57.07%和17.60%；对糖含量的抑制率分别为13.75%和8.20%，五寨黑豆显著高于合丰35。
Abstract:
Two cultivars with different resistance to Heterodera glycines，Wuzhaiheidou(resistant)and Hefeng 35(susceptible)were selected as materials to extract flavonoids in root by ethanol as organic solvent.The nematicidal activity of flavonoids extraction on the hatchability of cysts and the activity of J2 were determined.The results showed that flavonoids extracted from different varieties could effectively inhibit Heterodera glycines eggs hatched and kill J2.The inhibiting of Wuzhaiheidou flavonoids extraction increased 12.76%,9.03% and 9.37% more than Hefeng 35 when treated for 24,48 and 72 h.Treating 48 h，revised mortality of Hefeng 35 flavonoids extraction on J2 increased 7.0% than Wuzhaiheidou.The results of physiological toxicity of two varieties flavonoids extraction showed that:the flavonoids extraction of resistant and susceptible varieties could inhibite the glucose and protein content of Heterodera glycines.The inhibition of protein content were 57.07% and 17.60%,respectively.The inhibition of glucose content were 13.75% and 8.20%,respectively.

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备注/Memo

基金项目：农业部公益性行业科研专项（201103018，200903040-03）;国家自然科学基金（31171823）;现代农业产业技术体系（CARS-04-PS13)。

第一作者简介：段玉玺（1964-），男，教授，博导，主要从事植物病理学和植物线虫学教学和科研。E-mail:duanyx6407@163.com。

更新日期/Last Update: 2014-12-27