[1]张 拓,庞春杰,韩岚岚,等.大豆蚜基因组DNA四种提取方法的比较[J].大豆科学,2013,32(04):473-476.[doi:10.11861/j.issn.1000-9841.2013.04.0473]
　ZHANG Tuo,PANG Chun-jie,HAN Lan-lan,et al.Comparison of Four Methods for Genomic DNA Extraction from? Aphis glycines[J].Soybean Science,2013,32(04):473-476.[doi:10.11861/j.issn.1000-9841.2013.04.0473]
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大豆蚜基因组DNA四种提取方法的比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年04期 页码: 473-476 栏目: 出版日期: 2013-08-25
Title:
Comparison of Four Methods for Genomic DNA Extraction from? Aphis glycines
作者:
?张 拓; 庞春杰; 韩岚岚; 赵奎军
?东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
?ZHANG Tuo; PANG Chun-jie; HAN Lan-lan; ZHAO Kui-jun
?College of Agriculture，Northeast Agricultural University，Harbin 150030，China
关键词:
大豆蚜; 基因组DNA; 提取方法
Keywords:
Aphis glycines; Genomic DNA; Extraction method
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.04.0473
文献标志码:
A
摘要:
?以大豆蚜（Aphis glycines）为材料，比较分析了氯仿异戊醇抽提法、盐析法、KAc法及试剂盒法4种基因组DNA提取方法。结果表明：在提取大豆蚜基因组DNA的纯度上，氯仿-异戊醇抽提法和盐析法较高；提取所用时间由短到长依次为试剂盒提取法、盐析法、氯仿-异戊醇抽提法和KAc法。盐析法能够更快速有效地提取大豆蚜基因组DNA，是一种值得推广应用的基因组DNA提取方法,另外3种方法也有一定的优势，应根据试验条件和要求选择相应的基因组DNA提取方法。
Abstract:
?Using Aphis glycines as material，four genomic DNA extraction method including chloroform-isoamyl alcohol extraction，salting-out method，KAc and genomic DNA kit method were compared and analyzed. The purity of genomic DNA from chloroform-isoamyl alcohol extraction and salting-out method was better than the other two methods.And the total time of extraction in ascending order was genomic DNA kit method,salting-out method,the chloroform-isoamyl alcohol extraction and KAc.Salting-out method acted more quickly and effectively,the other three methods also had some advantages,therefore,the corresponding methods for genomic DNA extraction should be chosen according to the test conditions.

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备注/Memo

?基金项目:现代农业产业技术体系建设专项资金（CARS04）;公益性行业（农业）专项（201103002）。

更新日期/Last Update: 2014-02-17