[1]郑雅楠,陈乐,陈井生,等.休眠期大豆胞囊线虫体内关键酶活性变化[J].大豆科学,2013,32(04):526-529.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
　ZHENG Ya-nan,CHEN Le,CHEN Jing-sheng,et al.Changes of Related Enzyme Activities in Dormant Soybean Cyst Nematode,Heterodera glycines[J].Soybean Science,2013,32(04):526-529.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
点击复制

休眠期大豆胞囊线虫体内关键酶活性变化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年04期 页码: 526-529 栏目: 出版日期: 2013-08-25
Title:
Changes of Related Enzyme Activities in Dormant Soybean Cyst Nematode,Heterodera glycines
作者:
?郑雅楠1; 陈乐2; 陈井生3; 王媛媛2; 段玉玺2; 陈立杰2
?1.沈阳农业大学 林学院，辽宁 沈阳 110866；2.沈阳农业大学 植物保护学院，辽宁 沈阳 110866；3.黑龙江省农业科学院 大庆分院，黑龙江 大庆 163316
Author(s):
?ZHENG Ya-nan1; CHEN Le2; CHEN Jing-sheng3; WANG Yuan-yuan2; DUAN Yu-xi2; CHEN Li-jie2
?1.College of Forestry,Shenyang Agricultural University,Shenyang 110866,China;2.College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China;3.Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316,China
关键词:
大豆胞囊线虫; 休眠; 酶; 活性
Keywords:
Heterodera glycines; dormancy; enzyme; activity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.04.0526
文献标志码:
A
摘要:
?大豆胞囊线虫（Heterodera glycines,SCN）病是一种重要的大豆病害，以休眠胞囊越冬。为明确休眠期SCN的生理代谢变化，采用分光光度仪测定了休眠期SCN体内几种关键酶活性。结果表明：海藻糖酶（trehalase,TRE）、糖原磷酸化酶（glycogen phoshorylase,GP）、山梨醇脱氢酶（NAD-sorbitol dehydrogenase,NAD-SDH）、磷酸果糖激酶（phosphofructokinase,PFK）、丙酮酸激酶（pyruvate,PK）和果糖1，6-二磷酸羧醛酶（fructosel,6bisphosphotase,FBP）均与SCN休眠密切相关，进入休眠SCN体内海藻糖酶活性迅速升高，糖原磷酸化酶活性也显著升高，有利于海藻糖所需碳源的供给，而磷酸果糖激酶、丙酮酸激酶、果糖1，6-二磷酸羧醛酶和山梨醇脱氢酶4种酶活性均显著降低，休眠解除后活性回升。
Abstract:
?Soybean cyst nematode(SCN)disease(Heterodera glycines),which is a major disease of soybean,overwinters in its dormant stage.In order to find out the physiological metabolism of dormant SCN,the activities of dormancyrelated enzymes,including trehalase(TRE),glycogen phoshorylase(GP),NADsorbitol dehydrogenase(NADSDH),phosphofructokinase(PFK),pyruvate(PK)and fructose.1，6bisphosphotase(FBP）,were assayed in dormant SCN during different dormancy stage by spectrophotometer.The result showed that activities of these enzymes were closely related to dormancy of SCN,significantly increasing of TRE and GP activities when SCN entered into dormant stage,favored to supply of carbon source needed by TRE.But the activity of PFK,PK,FBP and NADSDH in dormant SCN were obviously lower than active SCN,then went up after dormancy broken.

相似文献/References:

[1]李 凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
　LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(04):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
[2]李泽宇,李肖白,陈井生,等.大豆品种（系）抗大豆胞囊线虫14号生理小种的抗性鉴定研究[J].大豆科学,2014,33(03):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
　LI Ze-yu,LI Xiao-bai,CHEN Jing-sheng,et al.Identification of Soybean Varieties for Resistance to Soybean Cyst Nematode Races 14[J].Soybean Science,2014,33(04):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
[3]袁翠平,赵洪锟,王玉民,等.利用SSR标记评价抗胞囊线虫野生大豆种质的遗传多样性[J].大豆科学,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
　YUAN Cui-ping,ZHAO Hong-kun,WANG Yu-min,et al.Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers[J].Soybean Science,2014,33(04):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
[4]李晓明,杜春梅,郑楠,等.芽孢杆菌BL-21、HNDF2对大豆胞囊线虫抑制效果的研究[J].大豆科学,2011,30(04):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
　LI Xiao-ming,DU Chun-mei,ZHENG Nan,et al.Inhibitory Effect of Bacillus BL-21,HNDF2 on Soybean Cyst Nematode[J].Soybean Science,2011,30(04):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
[5]郑雅楠,王媛媛,陈井生,等.大豆胞囊线虫热激蛋白基因Hsp70的克隆与原核表达[J].大豆科学,2012,31(02):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
　ZHENG Ya-nan,WANG Yuan-yuan,CHEN Jing-sheng,et al.Cloning and Prokaryotic Expression of Hsp70 from Soybean Cyst Nematode(Heterodera glycines)[J].Soybean Science,2012,31(04):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
[6]刘丹丹,段玉玺,陈立杰.酸类化合物对大豆胞囊线虫防效及寄主生长的影响[J].大豆科学,2012,31(02):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
　LIU Dan-dan,DUAN Yu-xi,CHEN Li-jie.Effect of Acid Compound on Soybean Cyst Nematode and Host Growth[J].Soybean Science,2012,31(04):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
[7]胡 新,许艳丽,LI Shu-xian,等.利用抗感品种混种防治大豆胞囊线虫效果的研究[J].大豆科学,2012,31(03):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
　HU Xin,XU Yan-li,LI Shu-xian,et al.Effect of Cultivar Mixture on Growth and Developoment of Soybean Inoculated with Soybean Cyst Nematode[J].Soybean Science,2012,31(04):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
[8]宋洁,许艳丽,姚钦,等.尿素对大豆胞囊线虫的抑制作用[J].大豆科学,2012,31(05):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
　SONG Jie,XU Yan-li,YAO Qin,et al.Suppression of Urea on Soybean Cyst Nematode[J].Soybean Science,2012,31(04):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
[9]胡海波,韩英鹏,滕卫丽,等.重组自交系群体对大豆胞囊线虫3号生理小种抗性与主要农艺性状的相关分析[J].大豆科学,2012,31(05):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]
　HU Hai-bo,HAN Ying-peng,TENG Wei-li,et al.Correlation between Resistance to Soybean Cyst Nematode Race No.3 and Major Agronomic Traits of Recombinant Inbred Lines[J].Soybean Science,2012,31(04):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]
[10]王大刚,卢为国,马 莹,等.新育成大豆品种对SMV和SCN的抗性评价[J].大豆科学,2009,28(06):949.[doi:10.11861/j.issn.1000-9841.2009.06.0949]
　WANG Da-gang,LU Wei-guo,MA Ying,et al.Evaluation of Resistance of Soybean Cultivars to Soybean Mosaic Virus and Soybean Cyst Nematode[J].Soybean Science,2009,28(04):949.[doi:10.11861/j.issn.1000-9841.2009.06.0949]
[11]孙晶双,郑雅楠,段玉玺,等.温度、光照和土壤含水量对大豆胞囊线虫休眠卵孵化的影响[J].大豆科学,2009,28(04):690.[doi:10.11861/j.issn.1000-9841.2009.04.0690]
　SUN Jing-shuang,ZHENG Ya-nan,DUAN Yu-xi,et al.Effect of Temperature，Illumination and Soil Water Content on Hatching of Dormant Eggs of Soybean Cyst Nematode[J].Soybean Science,2009,28(04):690.[doi:10.11861/j.issn.1000-9841.2009.04.0690]

备注/Memo

?基金项目:现代农业产业技术体系资助项目（CARS-04）；农业部公益性行业（农业）科研专项（200903040-03）。

更新日期/Last Update: 2014-04-04