[1]常云霞,徐克东,陈 璨,等.水杨酸对低温胁迫下大豆幼苗生长抑制的缓解效应[J].大豆科学,2012,31(06):927-931.[doi:10.3969/j.issn.1000-9841.2012.06.015]
　CHANG Yun-xia,XU Ke-dong,CHEN Can,et al.Salicylic Acid Mitigating the Inhibition of Low Temperature Stress to Soybean Seedlings[J].Soybean Science,2012,31(06):927-931.[doi:10.3969/j.issn.1000-9841.2012.06.015]
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水杨酸对低温胁迫下大豆幼苗生长抑制的缓解效应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年06期 页码: 927-931 栏目: 出版日期: 2012-12-30
Title:
Salicylic Acid Mitigating the Inhibition of Low Temperature Stress to Soybean Seedlings
文章编号:
1000-9841（2012）06-0927-05
作者:
常云霞1; 徐克东2; 陈 璨1; 陈 龙1
1.周口师范学院 生命科学系，河南 周口 466001；
2.周口师范学院 植物遗传与分子育种重点实验室，河南 周口 466001)
Author(s):
CHANG Yun-xia1; XU Ke-dong2; CHEN Can1; CHEN Long1
1.Department of Life Science,Zhoukou Normal University,Zhoukou 466001,Henan;?
2.Key Lab of Plant Genetics & Molecular Breeding,Zhoukou Normal University,Zhoukou 466001,Henan,China
关键词:
大豆幼苗; 低温胁迫; 水杨酸; 缓解效应
Keywords:
Salicylic acid; Soybean seedling; Low temperature stress; Mitigative effect
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.015
文献标志码:
A
摘要:
以中豆-31为材料，采用水培方法，研究了不同浓度的水杨酸(SA)对4℃低温胁迫下大豆幼苗生长的影响。结果表明，低温胁迫下，大豆幼苗叶绿素和可溶性蛋白含量、根系活力显著降低，可溶性糖、游离脯氨酸（Pro）、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）、过氧化物酶（POD）活性显著提高(P<0.01)；外施SA显著提高了低温胁迫下幼苗叶片的叶绿素、可溶性蛋白、可溶性糖和Pro含量，SOD、POD活性和根系活力，显著降低了膜脂过氧化产物MDA含量(P<0.01)。由此可见，外施SA可以通过提高大豆幼苗可溶性蛋白、可溶性糖、Pro含量以及SOD、POD活性来维持细胞膜的稳定性，降低膜脂过氧化伤害程度，从而缓解低温胁迫对幼苗生长的抑制，并以1.5 mmol·L-1外源SA效果最好。
Abstract:
Using soybean “Zhongdou-31” as the material,effects of different concentration of salicylic acid(SA) on the growth of soybean seedling under 4℃ low-temperature stress was studied by hydroponic culture.Under low-temperature stress,The content of chlorophyll,soluble proteins and the root activity were significantly decreased；the contents of proline,soluble sugar,MDA and the activities of SOD and POD were significantly increased(P<0.01).When the saybean seadlings were suffered from low-temperature stress for 2 days,exogenous SA significantly increased the contents of chlorophyll,soluble proteins,soluble sugar and proline,the root activity and the activities of SOD and POD,while significantly decreased MDA content(P<0.01).The results showed that exogenous SA could effectively keep cytomembrane stabilization.and reduce the damage from membrane lipid peroxidation.Hence,mitigate the harmful effects from low-temperature stress on plants,with the optimal concentration of 1.5 mmol·L-1 SA.

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备注/Memo

基金项目：河南省教育厅自然科学研究计划资助项目(2011B180057);周口师范学院生物化学与分子生物学重点学科建设项目。

第一作者简介：常云霞（1978-），女，硕士，讲师，主要从事植物抗性生理研究。E-mail：changyx618@126.com。
通讯作者：陈龙（1962-），男，教授，主要从事生物化学与分子生物学研究。E-mail：chenlongzg@126.com。

更新日期/Last Update: 2014-08-19