HAO Jian-jun,LU Huan,HUANG Chun-hua,et al.Influence of HSO3-、Cl-and Na+on Photosynthesis of Soybean Seedlings[J].Soybean Science,2012,31(05):855-857,860.[doi:10.3969/j.issn.1000-9841.2012.05.037]
HSO3-、Cl-和Na+对大豆幼苗光合作用的影响
- Title:
- Influence of HSO3-、Cl-and Na+on Photosynthesis of Soybean Seedlings
- 文章编号:
- 1000-9841(2012)05-0855-03
- Keywords:
- NaCl; NaHSO3; Soybean seedlings; Photosynthesis; Rubisco
- 分类号:
- S641.2
- 文献标志码:
- A
- 摘要:
- 以铁丰31大豆幼苗为试材,在大豆幼苗叶片完全展开时以一定浓度的NaCl和NaHSO3及清水喷施于叶片的正反两面,测定光合速率、可溶性糖、光合色素等生理指标,以探讨HSO3-、Cl-和Na+对光合作用的影响。结果表明:Na+对大豆幼苗叶片可溶性糖含量的增加、ATP合酶活性的提高、叶片Rubisco含量的增加均有一定的促进作用。Cl-在整个实验中作用不明显。 HSO3-对大豆幼苗叶片叶绿素a和叶绿素b的合成有显著促进作用;并能显著提高全链光合电子传递速率、ATP合酶活性,使光合磷酸化过程加强; HSO3-显著提高了Rubisco羧化活性及PEPC活力和PEPC比活力;并提高了Rubisco和PEPC含量,从而促进大豆幼苗的光合速率增加。
- Abstract:
- Soybean seedlings of Tiefeng No.31 was sprayed with NaCl and NaHSO3 solutions,respectively,and determined the photosynthetic rate,soluble sugar,photosynthetic pigment and other physiological indexes.Na+ increased soluble sugar content,ATP enzyme activity,and the content of Rubiscoase of soybean leaves.HSO3- promoted the synthesis of Chl a and Chl b;significantly increased the whole chain photosynthetic electron transfer rate and ATP synthase activity,strengthened the photosynthetic phosphorylation process,and speed up the process of the dark reactions of photosynthesis.Furthermore, HSO3- ?significantly improved the Rubiscoase carboxy change activity,PEPCase vitality as well as content of Rubisco and PEPC.Hence,promoted the photosynthetic rate of soybean seedlings.
参考文献/References:
[1]彭子模.亚硫酸氢钠对植物叶片气孔开度的调节作用[J].新疆师范大学学报(自然科学版),2000,19(2):41.(Peng Z M.Regulative function of NaHSO3 to stomatal exposure of plant blade[J].Journal of Xinjiang Normal University(Natural Sciences Edition),2000,19(2):41.) [2]周广业,王宏凯.光呼吸抑制剂亚硫酸氢钠的增产效果研究[J].土壤肥料,2000(6):35-38.(Zhou G Y,Wang H K.Research on the yield increasing effect of photorespiration inhibitor NaHSO3[J].Soil and Fertilizer,2000(6):35-38.) [3]张树芹,王宪泽,赵士杰,等.NaHSO3对小麦光合速率、光呼吸及籽粒氨基酸组成的影响[J].麦类作物,1999,19(1):44-46.(Zhang S Q,Wang X Z,Zhao S J,et al.The influence of NaHSO3 on wheat photosynthetic rate,light breathing and the grain amino acid composition[J].Journal of Triticeae Crops,1999,19(1):44-46.) [4]Wang H W,Shen Y G.How bisulfit enhances photosynthesis[J].Journal of Plant Physiology and Molecular Biology,2002,28(4):247-252. [5]Guo Y P,Hu M J,Zhou H F,et al.Low concentrations of NaHSO3 increase photosynthesis,biomass,and attenuate photo inhibition in Satsuma mandarin(Citrus unshiuMarc.)plants[J].Photosynthetica,2006,44(3):333-337. [6]Guo Y P,Peng Y,Lin M L,et al.Different pathways are involved in the enhancement of photo-synthetic rate by sodium bisulfite and benzy ladenine,a case study with strawberry(Fragaria ananassa Duch)plants[J].Plant Growth Regulation,2006,48:65-72. [7]郝建军,刘延吉.植物生理实验技术[M].沈阳:辽宁科学技术出版社,2001:20-23.(Hao J J,Liu Y J.Plant physiology experiment technology[M].Shenyang:Liaoning Science and Technology press,2001:20-23.) [8]张宪政.作物生理研究法(第一版)[M].北京:农业出版社,1992:15-16.(Zhang X Z.Crop physiology research(1st edition)[M].Beijing:Agricultural Press,1992:15-16.) [9]Arnon D I.Copper enzymes in isolated chloroplasts.Polyphenoloxidase in Beta vulgaris[J].Plant Physiology,1949,24(1):1-15. [10]陈启林,山仑,程智慧,等.低温弱光对黄瓜类囊体膜偶联状态的影响[J].西北农业大学学报,2000,28(16):6-11.(Cheng Q L,Shan L,Cheng Z H,et al.Influence of low temperature and weak light on cucumber thylakoid membrane coupling condition[J].Journal of Northwest Agricultural University,2000,28(16):6-11.) [11]王春梅,施定基,朱水芳,等.黄瓜花叶病毒对烟草叶片和叶绿体光合活性的影响[J].植物学报,2000,42(4):388-392.(Wang C M,Shi D J,Zhu S F,et al.Influence of cucumber mosaic virus on tobacco leaf and chloroplasts photosynthetically activity[J].Acta Botanic Sinica,2000,42(4):388-392.) [12]李合生.植物生理生化试验原理和技术(第一版)[M].高等教育出版社,2000.(Li H S.Principle and technology of plant physiological and biochemical experiments(1st edition)[M].Beijing:Higher Education Press,2000.) [13]Eckardt N A,Portis A R J.Heat denaturation profiles of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and Rubisco activase and the inability of Rubisco activase to restore activity of heat-denatured Rubisco[J].Plant Physiology,1997,113(1):243-248. [14]冯福生,马力耕.低温对玉米叶片PEP羧化酶及其调节特性的影响[J].植物生理报,1992,18(1):45-49.(Feng F S,Ma L G.Influence of low temperature on PEP carboxylase and its control characteristics of corn leaf[J].Journal of Plant Physiology,1992,18(1):45-49.) [15]董永华,史吉平,李广敏,等.干旱条件下乙烯对小麦幼苗PEP羧化酶活性的影响[J].河北农业大学学报,1995,18(3):26-30.(Dong Y H,Shi J P,et al.Influence of ethylene on wheat seedlings PEP carboxylase activity under drought conditions[J].Journal of Hebei Agricultural University,1995,18(3):26-30.)
相似文献/References:
[1]郭秋云,王 萍,刘兆普.大豆下胚轴不定芽对卡那霉素和氯化钠耐性的研究[J].大豆科学,2013,32(02):211.[doi:10.3969/j.issn.1000-9841.2013.02.016]
GUO Qiu-yun,WANG Ping,LIU Zhao-pu.Kanamycin and NaCl Tolerance of Adventitious Shoot Induced from Hypocotyl in Soybean[J].Soybean Science,2013,32(05):211.[doi:10.3969/j.issn.1000-9841.2013.02.016]
[2]张云月,王鹏,王丕武,等.大豆子叶节及苗期NaCl筛选试验研究[J].大豆科学,2011,30(03):438.[doi:10.11861/j.issn.1000-9841.2011.03.0438]
ZHANG Yun-yue,WANG Peng,WANG Pi-wu,et al.Susceptibility of Soybean Cotyledonary Node and Seedlings to NaCl Stress[J].Soybean Science,2011,30(05):438.[doi:10.11861/j.issn.1000-9841.2011.03.0438]
[3]徐明照,郭宁,朱贝贝.NaCl诱导大豆根尖细胞凋亡的细胞学效应[J].大豆科学,2010,29(01):61.[doi:10.11861/j.issn.1000-9841.2010.01.0061]
XU Ming-zhao,GUO Ning,ZHU Bei-bei.Cytological Effect on Apoptosis of Root Tip Cell of Soybean Induced by NaCl[J].Soybean Science,2010,29(05):61.[doi:10.11861/j.issn.1000-9841.2010.01.0061]
[4]王晗,于洋,郝建军.KHCO3和NaHSO3对大豆幼苗光合速率的影响[J].大豆科学,2008,27(03):546.[doi:10.11861/j.issn.1000-9841.2008.03.0546]
WANG Han,YU Yang,HAO Jian-jun.Effect of KHCO3?and NaHSO3 on Photosynthetic Rate of Soybean Seedlings[J].Soybean Science,2008,27(05):546.[doi:10.11861/j.issn.1000-9841.2008.03.0546]
备注/Memo
基金项目:辽宁省教育厅科学技术研究项目(2004A011)。