XU Ming-zhao,GUO Ning,ZHU Bei-bei.Cytological Effect on Apoptosis of Root Tip Cell of Soybean Induced by NaCl[J].Soybean Science,2010,29(01):61-63.[doi:10.11861/j.issn.1000-9841.2010.01.0061]
NaCl诱导大豆根尖细胞凋亡的细胞学效应
- Title:
- Cytological Effect on Apoptosis of Root Tip Cell of Soybean Induced by NaCl
- 文章编号:
- 1000-9841（2010）01-0061-03
- Keywords:
- Root of Soybean; NaCl; Apoptosis; Cytological effects
- 分类号:
- X171.5
- 文献标志码:
- A
- 摘要:
- 以大豆种子为试材，NaCl 溶液为诱导剂，分别对生长中的大豆根尖进行诱导，研究NaCl 溶液处理浓度和处理时间对根尖细胞凋亡的影响，并对凋亡细胞进行形态学显微观察和基因组DNA分子凝胶电泳分析。结果表明：利用NaCl溶液处理根尖细胞，能够引起细胞凋亡，细胞凋亡频率与处理溶液浓度和处理时间有关；在细胞发生凋亡时，凋亡中的细胞形态和结构在不同时期表现各异。证明利用NaCl溶液作为诱导剂处理生长中的大豆根尖细胞，能够引起细胞凋亡；凋亡细胞的形态和结构发生变化，DNA发生降解。
- Abstract:
- Treating soybean seed as experimental material，NaCl as the inducer，and by the way of inducing growing root tip of soybean，this research makes study on the response of root tip cell apoptosis of soybean to different NaCl treating hour and concentration.The microscopic observation on morphological apoptosis cell as well as gel-electrophoresis on root tip cell DNA of soybean showed：NaCl treatment on root tip cell would lead to apoptosis cell and its rate is related to NaCl concentration and treating time；the morphology and structure of apoptosis cell varied with stage.The research suggests that treatment upon root tip cell of soybean by NaCl as the inducer would lead to cell apoptosis，its morphology and structure varies and cell DNA degrades as well.
参考文献/References:
[1]翟中和，王喜忠，丁明孝.细胞生物学（第3版）[M].北京:高等教育出版社，2007:442-445.（Zhai Z H，Wang X Z，Ding M X.Cell biology(Third Edition)[M].Beijing:Higher Education Press，2007:442-445.） [2]张跃华，冉超，商艳艳，等.硝基苯对蚕豆根尖细胞凋亡的影响[J].生物学通报，2007，42(1):47-48.(Zhang Y H，Ran C，Shang Y Y，et al.Effects of nitrobenzene to apoptosis of Vicia?root tip cells[J].Biology Bulletin，2007，42(1):47-48.) [3]Golstein P.Cell death in us and others[J].Science，1998，281:1283. [4]Jabs T.Reactive oxygen intermediates as mediators of programmed cell death in plants and animals[J].Biochemical Pharmacology，1999，57(3):231-245. [5]肖军，高洁.高等植物细胞凋亡的诱因及生物学意义[J].山东农业大学学报(自然科学版)，2008，39(1):125-128.(Xiao J，Gao J.The inducement of apoptosis in higher plants and its biological significanc[J].Journal of Shandong Agricultural University(Natural Science)，2008，39(1):125-128.) [6]张旭红，林爱军，苏玉红，等.镉引起蚕豆叶片DNA损伤和细胞凋亡研究[J].环境科学，2006，27(4):787-793.(Zhang X H，Lin A J，Su Y H，et al.DNA damages and apoptosis induced by cell in the leaves of horse bean Vicia faba[J].Environmental Science，2006，27(4):787-793.) [7]潘建伟，陈虹，顾青.环境胁迫诱导的植物细胞程序性死亡[J].遗传，2002，24（3）:385-388.(Pan J W，Chen H，Gu Q.Environmental stress-induced programmed cell death of plant[J].Hereditas，2002，24（3）:385-388.) [8]马学军，舒跃龙，译.精编分子生物学实验指南[M].北京:科学出版社，2005:54-55，57-58.(Ma X J，Shu Y L,Translation.Experimental protocols in molecular biology guide[M].Beijing:Science Press，2005:54-55，57-58.) [9]Katsuhara M.Apoptosis-like cell death in barley roots under salt stress[J].Plant and Cell Physiology，1997，38:1091-1093.
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备注/Memo
基金项目：安徽农业大学细胞生物学重点课程开放性综合实验资助项目。