ZHOU Ke-qin,HAN Bing-jin,ZHANG Qiu-ying,et al.Effect of BRF-1 and BRF-2 on Root Rot and Seed Yield in Soybean[J].Soybean Science,2012,31(05):801-803.[doi:10.3969/j.issn.1000-9841.2012.05.024]
生防菌BRF-1和BRF-2对大豆根腐病和产量的影响
- Title:
- Effect of BRF-1 and BRF-2 on Root Rot and Seed Yield in Soybean
- 文章编号:
- 1000-9841(2012)05-0801-03
- 分类号:
- S435.651
- 文献标志码:
- A
- 摘要:
- BRF-1和BRF-2是中国科学院东北地理与农业生态农田分子生态学科组从大豆根际土壤筛选得到的2株革兰氏阳性生防细菌,为了检测它们对田间大豆根腐病的防治效果,2010年在沈阳军区龙镇农副业基地对BRF-1和BRF-2进行田间试验。结果表明:2株生防细菌均有效地抑制了大豆根腐病的发生,发病程度分别降低44.4%和8.8%,BRF-1菌株效果较好,在降低大豆根腐病病情指数的同时,能增加大豆植株花期、始粒期地上部和地下部干物重,提高大豆产量5.9%,是一株很有潜力的生防菌。
- Abstract:
- BRF-1(Paenibacillus Polymyxa)and BRF-2(Bacillus subtilis)are gram-positive bacteria obtained from soybean root rhizosphere.Dual-culture tests showed they had strong antagonism to many fungal pathogens,while they inhibited the development of plant diseases in vitro experiment.In 2010,a field trial with BRF-1 and BRF-2 was carried out in northern region of Heilongjiang Province to investigate their field performance in reducing disease incidence of soybean.Both BRF-1 and BRF-2 inhibited soybean root rot ?with a reduction in disease severity index by 44.4% and 8.8%,respectively.BRF-1 was a potential plant growth promoting bacteria in controlling soybean root rot,which increased above-ground dry matter and root dry matter accumulation at flowering and initial seed-filling stages,and thus enhanced seed yield by 5.9%.
参考文献/References:
[1]Bashan Y,De-Bashan L E.Bacteria/plant growth-promoting〔C〕//D Hillel.In Encyclopedia of soils in the environment.Oxford UK:Elsevier,2005,1:103-115.
[2]Bashan Y,Holgium G.Proposal for the division of plant growth-promoting rhizobacteria into two classifications:biocontrol-PGPB(plant-growth-promoting bacteria)and PGPB[J].Soil Biology and Biochemistry,1998,30:1225-1228.
[3]Oberh?nsli T,Défago G,Haas D.Indole-3-acetic acid(IAA)synthesis in the biocontrol strain CHAO of Pseudomona fluorescens:role of trytophan side chain oxidase[J].Journal of General Microbiology,1991,137:2273-2279.
[4]王光华,周克琴,张秋英,等.拮抗细菌BRF-1对几种植物病原真菌的抗生效果[J].中国生物防治,2003,19(2):73-77.(Wang G H,Zhou K Q,Zhang Q Y,et al.Antagonism of bacillus strain BRF-1 against plant pathogenic fungi[J].Chinese Journal of Biological Control,2003,19(2):73-77.)
[5]王光华,金剑,徐美娜,等.生防细菌BRF-1和BRF-2鉴定及生物学特征[J].中国生物防治,2007,23(1):49-54.(Wang G H,Jin J,Xu M N,et al.Identification and biological characteristics of biocontrol bacterial strains BRF-1 and BRF-2[J].Chinese Journal of Biological Control,2007,23(1):49-54.) [6]陈雪丽,王光华,金剑,等.多粘类芽孢杆菌BRF-1和枯草芽孢杆菌BRF-2对黄瓜和番茄枯萎病的防治效果[J].中国生态农业学报,2008,16(2):446-450.(Chen X L,Wang G H,Jin J,et al.Biocontrol effect of?Paenibaciuus polymyxa?BRF-1 and Bacillus subtilis BRF-2 on fusarium wilt disease of cucumber and tomato[J].Chinese Journal of Eco-agriculture,2008,16(2):446-450.) [7]Zhou K Q,Yamagishi M,Osaki M.?Paenibacillus BRF-1 has biocontrol ability against Phialophora gregata?disease and promotes soybean growth[J].Soil Science and Plant Nutrition,2008,54:870-875.
[8]Broedban T P,Baker K F,Waterworth Y.Bacteria and actinomycetes antagonistic to fungal root pathogens in Australian soil[J].Australian Journal Biological Science,1971,24:925-944.
[9]陈雪丽,郝再彬,王光华,等.多粘类芽孢杆菌BRF-1抗菌蛋白的分离纯化[J].中国生物防治,2007,23(1):156-159.(Chen X L,Hao Z B,Wang G H,et al.Purification of antifungal protein from?Paenibacillus polymyxa?BRF-1[J].Chinese Journal of Biological Control,2007,23(1):156-159.)
[10]姜莉莉,陈彦闯,辛明秀.枯草芽孢杆菌在防治植物病害上的应用及研究进展[J].安徽农学通报,2009,15(7):37-39.(Jiang L L,Chen Y C,Xin M X.Application and research advance in controlling plant fungous diseases by Bacillus subtilis[J].Anhui Agricultural Science Bulletin,2009,15(7):37-39.)〖HJ〗〖FL)〗〖LM〗
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备注/Memo
基金项目:中国科学院知识创新工程重要方向项目(KSCX2-YW-N-078);国家科技支撑计划项目(2009BADB3B06);中国科学院东北地理与农业生态研究所青年博士研究基金项目(KZCX3-SW-NA3-28)。