YAN Hong,WEI Yan-yang.Optimization on Cultivation Conditions of High Effective Chlorimuron-Ethyl Degrading Strain[J].Soybean Science,2012,31(05):804-807.[doi:10.3969/j.issn.1000-9841.2012.05.025]
高效氯嘧磺隆降解菌株培养条件的优化
- Title:
- Optimization on Cultivation Conditions of High Effective Chlorimuron-Ethyl Degrading Strain
- 文章编号:
- 1000-9841（2012）05-0804-04
- Keywords:
- Chlorimuron-ethyl; Chlorimuron-ethyl degrading strain; Cultivation conditions; Optimization
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以筛选出的3株高效氯嘧磺隆降解细菌（A2、A5和A6）为实验对象，研究碳源、氮源、pH、温度及氯嘧磺隆初始浓度对菌株生长的影响，确定其最适培养条件。A2、A5和A6生长的最适碳源为乳糖，氮源为蛋白胨。A2生长最适乳糖浓度为20 g·L-1，最适蛋白胨浓度为10 g·L-1，适宜pH值范围5.4~7.0，最适pH值6.0，生长的适宜温度范围25~36℃，最适温度28℃；A5和A6生长最适乳糖浓度为15 g·L-1，最适蛋白胨浓度为10 g·L-1，适宜pH值范围5.4~8.0，最适pH值5.4，生长的适宜温度范围25~36℃，最适温度28℃。株菌于最佳培养条件下，培养60 h，其最大菌密度（OD600）可分别达到1.802、1.804和1.887，分别是野生菌株的15.5、8.4和42.9倍。
- Abstract:
- The high effective Chlorimuron-ethyl degrading strains(A2,A5 and A6),which used in this study,were isolated and selected in early experiments.The effects of carbon sources,nitrogen source,pH,temperature,and chlorimuron-ethyl concentration on the growth were explored in this experiment.The results showed that the best carbon source and nitrogen source were lactose and peptone,respectively,for these three strains.As for A2,optimal concentration of lactose and peptone was 20 g·L-1 and 10 g·L-1,respectively.Suitable pH range of growth for A2 was 5.4-7.0,whereas the optimal pH was 6.0.Suitable temperature range of growth was 25-36℃,whereas the optimum temperature was 28℃.The suitable concentration of lactose and peptone for A5 and A6 were 15 g·L-1 and 10 g·L-1,respectively.Appropriate pH range was 5.4-8.0,and the optimal pH was 5.4.Suitable temperature range of growth for A5 and A6 was 25-36℃,and the optimum temperature was 28℃.The biggest bacterium density(OD600)of the three strains(A2,A5,and A6)could reach to 1.802,1.804 and 1.887,respectively,which were 15.5,8.4 and 42.9 times higher than wild strains,under the optimum culture condition for 60 h.
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备注/Memo
基金项目：哈尔滨理工大学2011年校级开放实验项目资助。