[1]裴晓峰,关大伟,李 俊,等.耐旱大豆根瘤菌的筛选及其接种效应[J].大豆科学,2012,31(03):420-424.[doi:10.3969/j.issn.1000-9841.2012.03.017]
　PEI Xiao-feng,GUAN Da-wei,LI Jun,et al.Screening of Drought-Tolerance Rhizobium and Its Influence on Soybean[J].Soybean Science,2012,31(03):420-424.[doi:10.3969/j.issn.1000-9841.2012.03.017]
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耐旱大豆根瘤菌的筛选及其接种效应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 420-424 栏目: 出版日期: 2012-03-25
Title:
Screening of Drought-Tolerance Rhizobium and Its Influence on Soybean
文章编号:
1000-9841（2012）03-0420-05
作者:
裴晓峰1; 关大伟2; 李 俊2; 曹凤明2; 李 力2; 马鸣超2; 朱宝成1
1.河北农业大学 生命科学学院 河北 保定 071001；
2.中国农业科学院 农业资源与农业区划研究所，北京 100081
Author(s):
PEI Xiao-feng1; GUAN Da-wei2; LI Jun2; CAO Feng-ming2; LI Li2; MA Ming-chao2; ZHU Bao-cheng1
1.College of Life Sciences,Agricultural University of Hebei,Baoding 071001,Hebei;
2.Institute of Agricultural Resources and Agricultural Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 10081,China
关键词:
根瘤菌; 耐旱性; 甜菜碱; 丙二醛; 超氧化物歧化酶; 叶绿素
Keywords:
Rhizobium; Drought-tolerance; Betaine; MDA; SOD; Chlorophyll
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.017
文献标志码:
A
摘要:
采用聚乙二醇(PEG)6000模拟干旱的试验方法，从分离自黄淮海地区的19株根瘤菌和2株参比菌株中初步筛选得到能够在该条件下生长良好的根瘤菌7株。进一步进行土壤盆栽干旱胁迫复筛试验，通过根瘤数量、大豆植株干重和含氮量等指标分析，获得了3株耐旱根瘤菌株B.japonicum 4788、B.japonicum 4792和B.japonicum USDA110；测定了在盆栽条件下接种B.japonicum 4792大豆植株耐旱性相关生理的指标，与未接种对照相比，接种根瘤菌的大豆叶片中甜菜碱、SOD酶和叶绿素的含量都有不同程度的增加，丙二醛的含量减少，表明接种根瘤菌可以提高大豆的耐旱性能。
Abstract:
Seven soybean Rhizobia strains were screened under simulated drought with PEG-6000 from 21 Rhizobia strains(including two reference strains),which were isolated from Huanghuaihai area.Subsequently,three strains of B.japonicum 4788,B.japonicum 4792 and B.japonicum USDA110 were selected by their performance of nodulation and nitrogen fixing under drought stress including nodule numbers,plant dry weights and total N contents in soil pot experiment with simulated drought environment.Several parameters related with drought resistance of plant were determined when B.japonicum 4792 was inoculated under the condition mentioned above.The results showed that the contents of betain,chlorophyll and SOD in plant were increased but MDA was decreased compared with the uninoculated control,which were beneficial for accumulation of drought-resistance materials and improvement of drought-resistant for soybean plant.

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备注/Memo

基金项目：现代农业产业技术体系建设专项资助项目(CARS-04)。

第一作者简介：裴晓峰（1983-），男，在读硕士，研究方向为微生物与生化药学。E-mail：pxf983621@163.com。
通讯作者：李俊（1965-），男，研究员，主要从事农业微生物学资源和生物固氮研究。E-mail：jli@caas.ac.cn。

更新日期/Last Update: 2014-08-16