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[1]李灿东,蒋洪蔚,郭泰,等.大豆耐旱选择群体基因型分析与株高QTL定位[J].大豆科学,2011,30(01):15-19.[doi:10.11861/j.issn.1000-9841.2011.01.0015]
　LI Can-dong,JIANG Hong-wei,GUO Tai,et al.QTL Identification of Plant Height and Analysis of Genotype to Soybean in Selection Population[J].Soybean Science,2011,30(01):15-19.[doi:10.11861/j.issn.1000-9841.2011.01.0015]

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大豆耐旱选择群体基因型分析与株高QTL定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷期数: 2011年01期页码: 15-19 栏目: 出版日期: 2011-02-25

Title:: QTL Identification of Plant Height and Analysis of Genotype to Soybean in Selection Population

文章编号:: 1000-9841（2011）01-0015-05

作者:: 李灿东¹; 2; 蒋洪蔚¹; 郭泰²; 王志新²; 吴秀红²; 郑伟²; 陈庆山³; 胡国华¹; 4; 1. 黑龙江省农垦科研育种中心，黑龙江哈尔滨 150090；
2. 黑龙江省农业科学院佳木斯分院，黑龙江佳木斯 154007
3.东北农业大学农学院，黑龙江哈尔滨 150030；
4.国家大豆工程技术研究中心，黑龙江哈尔滨 150050

Author(s):: LI Can-dong¹; 2; JIANG Hong-wei¹; GUO Tai²; WANG Zhi-xin²; WU Xiu-hong²; ZHENG Wei²; CHEN Qing-shan³; HU Guo-hua¹; 4; 1.Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090;
2. Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007;
3. College of Agriculture Northeast Agricultural University, Harbin 150030;
4.The National Research Center of Soybean Engineering and Technology, Harbin 150050, Heilongjiang, China

关键词:: 大豆; 耐旱性; 株高; QTL分析

Keywords:: Soybean; Drought tolerance; Plant height; QTL identification

分类号:: S565.1

DOI:: 10.11861/j.issn.1000-9841.2011.01.0015

文献标志码:: A

摘要:: 以红丰11为轮回亲本、Clark为供体亲本构建回交群体进行耐旱性鉴定，并对获得的选择群体进行全基因组SSR标记扫描，计算供体基因型导入频率，同时利用卡方测验检测偏分离SR位点，对株高进行QTL定位。结果表明：卡方测验检测到11个SSR偏分离位点（超导入）分布于8条连锁群；株高共定位8个QTL位点分布于A₁、C₂、E、F、L、M和O连锁群。在C₂连锁群的Satt305位点，耐旱供体等位基因显著性检测和株高QTL位点具有一致性；在E连锁群的Sat_136位点，F连锁群的Satt586和GMRUBP位点，L连锁群的Satt156位点，M连锁群的GMSL514位点均与有一致性，这些位点可能是控制大豆耐旱性的重要位点。

Abstract:: A primary backcross introgression soybean population was constructed by using Hongfeng 11 as recurrent parent and Clark as donor parent. After screened by drought stress, the genotype of selective population were obtained with the whole genome SSR markers, and the frequency of donor genes segments had been analyzed. QTLs of plant height was mapped by Chi-test analysis. In total, 11 SSR excessive introgression on 8 chromosomes were detected with χ² test. The QTL identification was conducted by one-way ANOVA (for single marker analysis, P<0.01). Eight QTLs of RPH were found in A₁, C₂, E, F, L, Mand O linkage groups. The QTL at Satt305 in C₂ linkage group was coincident with excessive introgression of χ² test. The same with Sat_136 in E linkage group, Satt586 and GMRUBP in F linkage group, Satt156 in L linkage group, GMSL514 in M linkage group. So these QTL were the essential loci for drought tolerance, and the foundation in fine mapping, cloning and molecular breeding of favorable genes related with drought tolerance.

参考文献/References:

[1]李原园, 李英能, 苏人琼, 等. 中国农业水危机及其对策[J]. 农业现代化研究, 1997 (3): 52-54. (Li Y Y, Li Y N, Su R Q, et al. Water crisis in agricultural sustainable development and its countermeasures in China[J]. Research of Agricultural Modernization, 1997(3): 52-54.) 

[2]山仑, 陈国良. 黄土高原旱地农业的理论与实践[M]. 北京：科学出版社, 1993: 125-129. (Shan L, Chen G L. Theory and practice for droughty region agriculture of Loess Plateau[M]. Beijing: Science Press, 1993: 125-129.)

[3]刘学义. 大豆抗旱性评定方法探讨[J]. 油料作物学报, 1986(4): 23-26. (Liu X Y. Discuss on evaluating method to drought-resistance of soybean[J]. Chinese Journal of Oil Crop Sciences, 1986(4): 23-26.)

[4]Sloane R J, Patterson R P, Carter T E. Field drought tolerance of soybean plant introduction[J]. Crop Science, 1990, 30: 118-123.

[5]Liu F L, Andersen M N, Jacobsen S E. Stomatal control and water use efficiency of soybean (Glycine maxMerrill) during progressive soil drying[J]. Environmental and Experimental Botany, 2004, 54: 1-8.

[6]Hudak C M, Patterson R P. Vegetative growth analysis of a drought resistant soybean plant introduction[J]. Crop Science, 1995, 35: 464-471.

[7]Garcia A, Gonzalez M C. Morphological markers for the early selection of drought tolerant rice varieties[J]. Cultivate Tropical, 1997, 18: 47-50.

[8]黄培堂译. 分子克隆三[M]. 北京: 科学出版社, 2002: 363-365. (Huang P T， trans. Molecular Cloning 3rd[M]. Beijng: Science Press, 2002: 363-365.)

[9]贺超英. 大豆微卫星遗传图谱构建及抗病相关基因的克隆与研究[D]. 北京：中国科学院遗传与发育生物学研究所, 2001：35-38. (He C Y. Construction of soybean genetic map by SSR and its application in cloning of disease-resistance genes[D]. Beijing：Institute of Genetics and Developmental Biology of Chinese Academy of Sciences, 2001：35-38.)

[10]刘莹, 蔡祈明. 大豆根系形态及苗期耐旱根系性状的研究[J]. 河北工程大学学报, 2009, 26(4): 59-61. (Liu Y, Cai Q M. Root morphology and root traits related to drought tolerance at vegetation stage in soybean[J]. Journal of Hebei University of Engineering, 2009, 26(4): 59-61.)

[11]刘莹, 张孟臣. 夏大豆产量相关根系性状及QTJL定位[J]. 河北工程大学学报, 2010, 27(1): 65-69. (Liu Y, Zhang M C. Study on root trait related yield in soybean growing in summer and QTL mapping[J]. Journal of Hebei University of Engineering, 2010, 27(1): 65-69.)

[12]刘莹, 盖钧镒, 吕慧能,等. 大豆耐旱种质鉴定和相关根系性状的遗传与QTL定位[J]. 遗传学报, 2005, 32(8): 855-863. (Liu Y, Gai J Y, Lv H N, et al. Identification of drought tolerant germplasm at seeding stage and QTL mapping of related root traits in soybean[J]. Journal of Genetics and Genomics, 2005, 32(8): 855-863.)

[13]Specht J E, Chase K, Macrander M, et al. Soybean response to water: A QTL analysis of drought tolerance[J]. Crop Science, 2001, 41(2): 493-509. 

[14]Mian M A R, Ashley D A, Boerma H R. An additional QTL for water use efficiency in soybean[J]. Crop Science, 1998, 38: 390-393. 

[15]Mian M A R, Bailey M A, Ashley D A. Molecular markers associated with water use efficiency and leaf ash in soybean[J]. Crop Science, 1996, 36: 1252-1257.

[16]杨剑平, 陈学珍, 王文平,等. 大豆实验室PEG6000模拟乾旱体系的建立[J]. 中国农学通报, 2003, 19(3): 65-68. (Yang J P, Chen X Z, Wang W P, et al. The establishment of the simulated system of drought for soybean in laboratory[J]. Chinese Agricultural Science Bulletin, 2003, 19(3): 65-68.)

[17]李甜, 朱延姝, 张晓萍, 等. 不同抗旱类型大豆品种生理特征的探讨[J]. 东北师范大学学报, 1999(2): 122-124. (Li T, Zhu Y S, Zhang X P, et al. The discussion for physiological in different drought-resistance of soybean[J]. Journal of Northeast Normal University, 1999(2): 122-124.)

[18]李贵全, 杜维俊, 孔照胜, 等. 不同大豆品种抗旱生理生态的研究[J]. 山西农业大学学报, 2000, 20(3): 197-200. (Li G Q, Du W J, Kong Z S, et al. Study on the relationships between drought-resistance and physiological and ecological indicators in different soybean varieties[J]. Journal of Shanxi Agricultural University, 2000, 20(3): 197-200.)

[19]李灿东, 蒋洪蔚, 张闻博,等. 大豆荚粒性状QTL定位[J]. 分子植物育种, 2008, 6(6): 1-10. (Li C D, Jiang H W, Zhang W B, et al. QTL analysis of seed and pod traits in soybean[J]. Molecular Plant Breeding, 2008, 6(6): 1-10.)

[20]Xu J L, Lafitte H R, Gao Y M, et al. QTLs for drought escape and tolerance identified in a set of random introgression lines of rice[J]. Theoretical and Applied Genetics, 2005,111: 1642-1650.

[21]郑天清, 徐建龙, 傅彬英,等. 遗传搭车与方差分析在水稻定向选择群体的抗旱性位点分析中的初步应用[J]. 作物学报, 2007, 33(5): 799-804. (Zheng T Q, Xu J L, Fu B Y, et al. Application of genetic hitch-hiking and ANOVA in identification of loci for drought tolerance in populations of rice from directional selection[J]. Acta Agronomica Sinica, 2007, 33(5): 799-804.)

[22]蒋洪蔚, 李灿东, 刘春燕, 等. 大豆导入系群体芽期耐低温位点的基因型分析及QTL定位[J]. 作物学报, 2009, 35(7): 1268-1273. (Jiang H W, Li C D, Liu C Y, et al. Genotype analysis and QTL mapping for tolerance to low temperature in germination by introgression lines in soybean[J]. Acta Agronomica Sinica, 2009, 35(7): 1268-1273.)

[23]Li Z K, Fu B Y, Gao Y M, et al. Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativaL.)[J]. Plant Molecular Biology, 2005, 59: 33-52.

[24]李灿东, 蒋洪蔚, 张闻博, 等. 大豆定向选择群体耐旱性位点基因型分析及QTL定位[J]. 中国农学通报, 2009, 25(3):285-292. (Li C D, Jiang H W, Zhang W B, et al. Genotype and QTL analysis of drought tolerance loci for directional population in soybean[J]. Chinese Agricultural Science Bulletin, 2009, 25(3):285-292.)

[22]王慧，喻德跃，吴巧娟，等. 大豆对斜纹夜蛾抗生性基因的微卫星标记(SSR)的研究[J]. 大豆科学，2004，23（2）：91-95. (Wang H, Yu D Y, Wu Q J,et al. Characterization of resistance genes to cotton worm with SSR markers in soybean[J]. Soybean Science, 2004, 23(2)：91-95.)

[23]Komatsu K, Okuda S, Takahashi M, et al. QTL mapping of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean[J]. Crop Science, 2005, 45：2044-2048.

[24]刘华，王慧，李群，等. 大豆对斜纹夜蛾抗性的遗传分析及相关QTL的定位[J]. 中国农业科学，2005, 38(7)：1369-1372. (Liu H, Wang H, Li Q, et al. Inheritance analysis and mapping QTLs related to cotton worm resistance in soybean[J]. Scientia Agricultura Sinica, 2005, 38(7)：1369-1372. )

[25]付三雄，王慧，吴娟娟，等. 应用重组自交系群体定位大豆抗虫QTL[J]. 遗传，2007, 29(9)：1139-1143. (Fu S X, Wang H, Wu J J, et al. Mapping insect resistance QTLs of soybean with RIL population[J]. Hereditas, 2007, 29(9)：1139-1143.) 

[26]詹秋文，盖钧镒. 大豆种质资源对斜纹夜蛾(Prodenia Litura)抗性的鉴定[J]. 应用与环境生物学报，2000,6(1):18-23. (Zhan Q W，Gai J Y. Evaluation of resistance of soybean germplasm to cotton worm (Prodenia litura, Fabricius)[J]. Chinese Journal of Applied & Environmental Biology, 2000, 6(1)：18-23.)

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备注/Memo

基金项目：农业重大专项资助项目（2009ZX08009-13B）；农业公益性行业科研专项资助项目（200903003）。

第一作者简介：李灿东(1984-)，男，硕士，研究方向为大豆遗传育种及生物技术。E-mail: licandong@126.com。?

通讯作者：胡国华（1969-），男，研究员，主要从事大豆遗传育种研究。E-mail: Hugh757@vip.163.com。

更新日期/Last Update: 2014-09-11