[1]陈晟,郭丽琼,宋景深,等.T5代γ-亚麻酸转基因大豆的遗传稳定性分析[J].大豆科学,2012,31(01):24-28.[doi:10.3969/j.issn.1000-9841.2012.01.006]
　CHEN Sheng,GUO Li-qiong,SONG Jing-shen,et al.Genetic Stability Analysis of the Fifth Generation of Transgenic Soybeans Expressing γ-linolenic Acid[J].Soybean Science,2012,31(01):24-28.[doi:10.3969/j.issn.1000-9841.2012.01.006]
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T5代γ-亚麻酸转基因大豆的遗传稳定性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年01期 页码: 24-28 栏目: 出版日期: 2012-02-25
Title:
Genetic Stability Analysis of the Fifth Generation of Transgenic Soybeans Expressing γ-linolenic Acid
文章编号:
1000-9841（2012）01-0024-05
作者:
陈晟1; 郭丽琼1; 2; 宋景深1; 林俊芳1; 2
1.华南农业大学 食品学院；
2.华南农业大学 生物质能研究所，广东 广州 510642
Author(s):
CHEN Sheng1; GUO Li-qiong1; 2; SONG Jing-shen1; LIN Jun-fang1; 2
1.College of Food Science,South China Agricultural University;
2.Institute of Biomass,South China Agricultural University,Guangzhou 510642,Guangdong,China
关键词:
遗传稳定性; 转基因大豆; γ-亚麻酸; △6-脂肪酸脱氢酶
Keywords:
Genetic stability; Transgenic soybeans; γ-linolenic acid; △6-fatty acid desaturase
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.006
文献标志码:
A
Abstract:
The genetic stability of foreign gene and separation of △6-fad and bar gene were observed in the 5th generation of transgenic soybeans expressing γ-linolenic acid.By PCR amplification and resistance testing of selective marker gene,the genome of transgenic soybeans TS824 and TS825 were integrated △6-fad gene(marker free); TS81, TS83, TS85,TS87,TS814,TS816 and TS818 were integrated bar gene;TS88,TS89,TS810,TS813,TS815,TS816,TS817,TS819 and TS820 were integrated both △6-fad gene and bar gene;neither △6-fad gene nor bar gene was detected in TS82 and TS86.The results showed that △6-fad gene was stably maintained in the genome of part of transgenic soybeans,and △6-fad and bar gene of transgenic soybeans TS824 and TS825 were separated.

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项课题（2009ZX0004-007B）；广东省科技攻关项目（2005B20101009，2009A020102004）。

第一作者简介：陈晟（1988-），男，在读硕士，研究方向为转基因大豆及生物技术。E-mail：ngcs1988@126.com。
通讯作者：林俊芳（1962-），男，研究员，博士生导师，研究方向为生物活性物质与生物炼制。E-mail：linjf@scau.edu.cn。

更新日期/Last Update: 2014-08-15