[1]钟灿,肖深根,朱保葛,等.菜用大豆高效胚尖离体再生基因型筛选[J].大豆科学,2012,31(01):9-12.[doi:10.3969/j.issn.1000-9841.2012.01.003]
　ZHONG Can,XIAO Shen-gen,ZHU Bao-ge,et al.Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean[J].Soybean Science,2012,31(01):9-12.[doi:10.3969/j.issn.1000-9841.2012.01.003]
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菜用大豆高效胚尖离体再生基因型筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年01期 页码: 9-12 栏目: 出版日期: 2012-02-25
Title:
Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean
文章编号:
1000-9841（2012）01-0009-04
作者:
钟灿1; 肖深根1; 朱保葛3; 薛仁镐4; 卫志明2; 朱木兰2
1.湖南农业大学 园艺园林学院，湖南 长沙 410128；
2.中国科学院 上海生命科学研究院，植物生理生态研究所，国家植物基因研究中心（上海）， 上海 200032；
3.中国科学院 遗传与发育生物学研究所，北京 100101
4.青岛农业大学 生命科学学院，山东 青岛 266109
Author(s):
ZHONG Can1; XIAO Shen-gen1; ZHU Bao-ge3; XUE Ren-gao4; WEI Zhi-ming2; ZHU Mu-lan2
1.College of Horticulture and Gardening,Hunan Agricultural University,Changsha 410128,Hunan;
2.Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,National Center for Plant Gene Research (Shanghai)Chinese Academy of Sciences（CAS）, Shanghai 200032;
3.Institute of Genetics and Developmental Biology,CAS,Beijing 100101;
4.College of Life and Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China
关键词:
菜用大豆; 胚尖; 基因型筛选; 离体再生
Keywords:
Vegetable-type soybean; Embryonic tips; Selection of genotype; Regeneration
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.003
文献标志码:
A
摘要:
以华东地区4个主栽菜用大豆品种（交大05-133、交大02-89、沪宁96-10、青酥二号）的胚尖为起始外植体，研究消毒方法、预培养天数、6-BA浓度和培养基组合等对不定芽的诱导和伸长的影响。结果表明：用0.1% HgCl2消毒10 min后配合5%的NaClO消毒5 min，消毒效果最佳，胚尖活力好，且适用于各个品种；预培养时间为2 d，6-BA浓度为3.0 mg·L-1，有利于菜用大豆不定芽的诱导；1.0 mg·L-16-BA+0.1 mg·L-1 NAA培养基组合有利于增加有效不定芽数；0.05 mg·L-1 6-BA+0.1 mg·L-1IBA培养基组合有利于不定芽的伸长；交大05-133为最佳胚尖离体再生基因型，其分化频率为90.86%，诱导15 d后外植体平均不定芽数为4.65个，不定芽平均长度为1.38 cm。
Abstract:
To study the effect of sterilization,pre-culture days,different concentrations of 6-BA and medium formulations on induction and elongation of adventitious buds,embryonic tips from four vegetable-type soybean varieties ‘Jiaoda 05-133’,‘Jiaoda 02-89’,‘Huning 96-10’ and ‘Qingsuerhao’ were used as the initial explants.The results showed that the optimal sterilization was using 0.1% HgCl2for 10 min and 5% NaClO for 5 min.The optimal adventitious buds induction formulation was MSB5base medium with 3.0 mg·L-16-BA,and the optimal pre-culture days of embryonic tips was 2 d.The combination of 1.0 mg·L-16-BA and 0.1 mg·L-1NAA benefited to produce many adventitious buds.The combination of 0.05 mg·L-16-BA and 0.1 mg·L-1IBA benefited to the elongation of adventitious buds.Among the four varieties,the embryonic tips from Jiaoda 05-133 is the best one for regeneration,with 90.86% of adventitious buds induction rate,4.65 of mean number of adventitious buds and 1.38 cm of mean length of adventitious buds after culture for 15 d.

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备注/Memo

基金项目：转基因生物新品种培育重大专项资助项目（2009ZX08010-009B，2009ZX08004-009B）。

第一作者简介：钟灿（1986-），女，硕士，研究方向为蔬菜生物技术。E-mail:canzhong651@163.com。
通讯作者：朱木兰(1968-），女，副研究员，从事植物分子遗传研究。E-mail:mlzhu@sippe.ac.cn。

更新日期/Last Update: 2014-08-14