[1]来永才,李炜,林红,等.不同组配方式下大豆异黄酮含量的遗传分析[J].大豆科学,2011,30(04):580-584.[doi:10.11861/j.issn.1000-9841.2011.04.0580]
　LAI Yong-cai,LI Wei,LIN Hong,et al.Genetic Analysis on Isoflavone Content of Different Soybean Hybridization[J].Soybean Science,2011,30(04):580-584.[doi:10.11861/j.issn.1000-9841.2011.04.0580]
点击复制

不同组配方式下大豆异黄酮含量的遗传分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年04期 页码: 580-584 栏目: 出版日期: 2011-08-25
Title:
Genetic Analysis on Isoflavone Content of Different Soybean Hybridization
文章编号:
1000-9841（2011）04-0580-05
作者:
来永才1; 李炜1; 林红2; 肖佳雷1; 李琬1; 杨雪峰2; 毕影东1
1.黑龙江省农业科学院 耕作栽培研究所，黑龙江 哈尔滨 150086；
?2.黑龙江省农业科学院 育种研究所，黑龙江 哈尔滨150086
Author(s):
LAI Yong-cai1; LI Wei1; LIN Hong2; XIAO Jia-li1; LI Wan1; YANG Xue-feng2; BI Ying-dong1
1.Institute of Crop Tillage and Cultivation,Heilongjiang Acadomy of Agriculture Sciences, Harbin 150006;?
2.Institute of Breeding, Heilongjiang Acddemy of Agriculture Sciences,Harbin 150006, Heilongjiang， China
关键词:
大豆[Glycine max(L.)Merr.]; 异黄酮; 配合力
Keywords:
Soybean[Glycine max(L.)Merr.]; Isoflavone; Combining ability
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0580
文献标志码:
A
摘要:
选用野生大豆、栽培大豆及种间高世代材料按异黄酮含量不同配制杂交组合，对亲本和后代大豆籽粒异黄酮含量进行遗传分析。结果表明:大豆母本配合力F值均达到显著水平，将方差分解成母本组P1(高、中、低)和父本组P2(高、中、低)亲本的一般配合力及特殊配合力方差并进行F测验后发现，母本组一般配合力方差F测验达到了显著水平，表明杂交组合所测性状受到亲本基因加性效应影响，亲本不同组合的一般配合力和特殊配合力差异较大。33个大豆亲本组合中，特殊配合力相对效应值的变幅为-28.6%～32.5%，效应值最高的组合（龙品05-277×野01-177）为32.5%，该组合可作为实现高异黄酮育种目标的重要参考组合。在大豆高异黄酮育种中，亲本最好采用高×高类型配置组合。
Abstract:
In order to broaden genetic basis of soybean and enhance breeding efficiency,36 hybridized combinations were designed according to isoflavone content(high and low).Heritability and combining ability effect of isoflavone content was analyzed.The difference of female parent combining ability level was significant with F test.General combining ability variance of female parent was significant with F test when the variance was resolved into female parent group and male parent group according to isoflavone content(high,middle and low).Both additive and non-additive effect of parents for isoflavone content were exist in these combinations.Range of specific combining ability was -28.6%～32.5%,with Longpin 05-277×Wild 01-177 the highest.For high isoflavone breeding,parents combination of high×high should be selected.

参考文献/References:

[1]Messina M J,Wood C E.Soy isoflavones,estrogen therapy,and breast cancer risk:analysis and commentary[J].Nutrition Journal,2008,7(1):17-28.

[2]Song W O,Chun O K,Hwang I,et al.Soy isoflavones as safe functional ingredients[J].Journal of Medicinal Food,2007,10(4):571-80.

[3]Doerge D R,Sheehan D M.Goitrogenic and estrogenic activity of soy isoflavones[J].Environmental Health Perspectives,2002,110(3):349-53.

[4]Kaufman P B,Duke J A,Brielmann H,et al.A comparative survey of leguminous plants as sources of the isoflavones,genistein and daidzein:implications for human nutrition and health[J].The Journal of Alternative and Complementary Medicine,1997,3(1):7-12.

[5]Aoki T,Akashi T,Ayabe S.Flavonoids of leguminous plants:structure,biological activity,and biosynthesis[J].Journal of Plant Research,2000,113(4):475-88.

[6]Yu O,Jung W,Shi J,et al.Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues[J].Plant physiology,2000,124(2):781-49.

[7]王春娥,赵团结,盖钧镒.中国大豆资源异黄酮含量及其组分的遗传变异和演化特征[J].中国农业科学,2010,43(19):1-11.(Wang C E,Zhao T J,Gai J Y.Genetic variability and evolutionary peculiarity of isoflavone content and its components in soybean germplasm from China[J].Scientia Agricultura Sinica,2010,43(19):1-11.)

[8]林红,齐宁,李向华,等.黑龙江省野生大豆资源考察研究[J].中国油料作物学报,2006,28（4）:427-430.(Lin H,Qi N,Li X H,et al.New progress on wild soybean survey in Heilongjiang province[J].Chinese Journal of Oil Crop Sciences,2006,28（4）:427-430.)

[9]Lers A,Burd S,Lomaniec E,et al.The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation[J].Plant Molecular Biology,1998,36(6):847-856.

[10]Bolwell G P,Bell J N,Cramer C L,et al.L-Phenylalanine ammonia-lyase from phaseolus vulgaris[J].European Journal of Biochemistry,2005,149(2):411-419.

[11]Henderson S J,Friend J.Increase in PAL and lignin-like compounds as race-specific resistance responses of potato tubers to Phytophthora?infestans[J].Journal of Phytopathology,2008,94(4):323-334.

[12]来永才,李炜,毕远林,等.黑龙江省野生大豆高异黄酮新种质创新利用研究Ⅲ大豆种间杂交F1代异黄酮的遗传规律和杂种优势的研究[J].大豆科学,2008,27（2）:212-214.(Lai Y C,Li W,Bi Y L,et al.Innovation and utilization of new high isoflavone resource of wild soybean in Heilongjiang Ⅲ Hereditary law of F1isoflavone content and heterosis in soybean interspecific cross[J].Soybean science,2008,27（2）:212-214.)

[13]Griffing B.Concept of general and specific combining ability in relation to diallel crossing systems[J].Australian Journal of Biological Sciences 1956,9(4):463-493.

[14]Betrán F J,Ribaut J M,Beck D,et al.Genetic diversity,specific combining ability,and heterosis in tropical maize under stress and nonstress environments[J].Crop Science,2003,43:797-806.

[15]宋启建,盖钧镒.大豆杂种后代蛋白质和脂肪含量的配合力研究[J].作物学报,1991,17（2）:128-134.(Song Q J,Gai J Y.An analysis of genetic ability and combining ability for protein and oil content in soybean interspecific cross[J].Acta Agronomica Sinica,1991,17（2）:128-134.)

[16]崔润芝,田保明.夏大豆产量性状的遗传力和配合力分析[J].华北农学报,1994,9（4）:59-64.(Cui R Z,Tian B M.An analysis of genetic ability and combining ability for yield characters in summer soybean[J].Acta Agriculturac Boreali-Sinica,1994,9（4）:59-64.)

[17]Henderson C R.Specific and general combining ability[M].Heterosis,Iowa State College Press,Ames,IA,1950:352-70.

[18]孙君明,常汝镇.大豆籽粒中异黄酮含量的遗传初步分析[J].中国农业科学,2002,35（1）:16-21.(Sun J M,Chang R Z.Genetic analysis on isoflavone content in soybean beeds[J].Scientia Agricultura Sinica,2002,35（1）:16-21.)

[19]梁慧珍,李卫东,方宣钧,等.大豆异黄酮及其组分含量的配合力和杂种优势[J].中国农业科学,2005,38（10）:2147-2152.(Liang H Z,Li W D,Fang X J,et al.Soybean isoflavone and its components among the soybean varieties[Glycine max(L.)Merr.][J].Scientia Agricultura Sinica,2005,38（10）:2147-2152.)


相似文献/References:

[1]张晶莹,孙君明,李 斌,等.高效液相色谱法快速检测饲料中大豆异黄酮的主要组分含量[J].大豆科学,2013,32(04):530.[doi:10.11861/j.issn.1000-9841.2013.04.0530]
　ZHANG Jing-ying,SUN Jun-ming,LI Bin,et al.A Rapid Method for Determination of Soybean Isoflavone Components in Feeds by HPLC[J].Soybean Science,2013,32(04):530.[doi:10.11861/j.issn.1000-9841.2013.04.0530]
[2]解晚晴,吕玉翠,孔祥珍,等.高温加热豆浆对其异黄酮成分的影响[J].大豆科学,2014,33(01):62.
　XIE Wan-qing,LYU Yu-cui,KONG Xiang-zhen,et al.Effect of Heat Treatment on Soy Isoflavones in Soy Milk[J].Soybean Science,2014,33(04):62.
[3]梁晓芳,王步军.大豆中异黄酮和皂苷的提取、制备研究进展[J].大豆科学,2014,33(01):128.[doi:10.11861/j.issn.1000-9841.2014.01.0128]
　LIANG Xiao-fang,WANG Bu-jun.Research Progress of Extraction and Preparation Methods of Soybean Isoflavones and Soyasaponins[J].Soybean Science,2014,33(04):128.[doi:10.11861/j.issn.1000-9841.2014.01.0128]
[4]尹学哲,金延华,王玉娇,等.大豆异黄酮和皂甙对D-氨基半乳糖所致肝损伤小鼠肝脏抗氧化活力的影响[J].大豆科学,2014,33(01):139.[doi:10.11861/j.issn.1000-9841.2014.01.0139]
　YIN Xuezhe,JIN Yanhua,WANG Yujiao,et al.Effects of Soy Isoflavones and Saponins on Hepatic Antioxidative Activity of Mice with Acute Liver Injury Induced by D galactoamine[J].Soybean Science,2014,33(04):139.[doi:10.11861/j.issn.1000-9841.2014.01.0139]
[5]许惠仙,汪霞,金延华,等.大豆异黄酮和皂甙对肝癌前病变大鼠血清标志酶及抗氧化活性的影响[J].大豆科学,2012,31(01):124.[doi:10.3969/j.issn.1000-9841.2012.01.028]
　XU Hui-xian,WANG Xia,JIN Yan-hua,et al.Effect of Soybean Isoflavones and Saponins on Serum Marker Enzymes and Antioxidative Activities of Rat with Hepatic Preneoplasia[J].Soybean Science,2012,31(04):124.[doi:10.3969/j.issn.1000-9841.2012.01.028]
[6]毕影东,李 炜,林 红,等.大豆种间杂交异黄酮含量的杂种优势分析[J].大豆科学,2012,31(02):203.[doi:10.3969/j.issn.1000-9841.2012.02.009]
　BI Ying-dong,LI Wei,LIN Hong,et al.Heterosis of Soybean Isoflavone Content in Interspecific Hybridization[J].Soybean Science,2012,31(04):203.[doi:10.3969/j.issn.1000-9841.2012.02.009]
[7]赵文玺,汪 霞,金爱花,等.大豆异黄酮和皂甙对大鼠肝癌诱发初期肝脏氧化应激的干预作用[J].大豆科学,2012,31(04):675.[doi:10.3969/j.issn.1000-9841.2012.04.032]
　ZHAO Wen-xi,WANG Xia,JIN Ai-hua,et al.Effect of Soybean Isoflavones and Saponins on Hepatic Oxidative Stress of Rats in Early Stage of Hepatocarcinogenesis[J].Soybean Science,2012,31(04):675.[doi:10.3969/j.issn.1000-9841.2012.04.032]
[8]张 应,李向华,肖鑫辉,等.重庆不同类型大豆异黄酮含量与品质性状的测定与分析[J].大豆科学,2011,30(01):67.[doi:10.11861/j.issn.1000-9841.2011.01.0067]
　ZHANG Ying,LI Xiang-hua,XIAO Xin-hui,et al.Detection and Analysis of Isoflavone Content and Quality Traits of Different Soybean Types in Chongqing[J].Soybean Science,2011,30(04):67.[doi:10.11861/j.issn.1000-9841.2011.01.0067]
[9]杨雪峰,李文滨,齐 宁,等.野生大豆蛋白质、脂肪含量与异黄酮含量的相关性研究[J].大豆科学,2011,30(01):161.[doi:10.11861/j.issn.1000-9841.2011.01.0161]
　YANG Xue-feng,LI Wen-bin,QI Ning,et al.Correlation Between Protein, Oil and Isoflavone Content in Wild Soybean[J].Soybean Science,2011,30(04):161.[doi:10.11861/j.issn.1000-9841.2011.01.0161]
[10]牛丽颖,蔡广华,刘敏彦,等.淡豆豉中异黄酮提取工艺的优化研究[J].大豆科学,2011,30(01):164.[doi:10.11861/j.issn.1000-9841.2011.01.0164]
　NIU Li-ying,CAI Guang-hua,LIU Min-yan,et al.Research on Optimization of Isoflavone Extracting Technique from Semen Sojae Praeparatum[J].Soybean Science,2011,30(04):164.[doi:10.11861/j.issn.1000-9841.2011.01.0164]

备注/Memo

基金项目：国家科技支撑计划资助项目（2006BAD13B05-5）；黑龙江省博士后基金资助项目 (LBH-Z06233)； 哈尔滨市科技创新人才研究专项基金资助项目（2007RFXYN053）。

第一作者简介：来永才(1964-)，男，研究员，博士，从事野生大豆种质资源创新及利用研究。E-mail: yame0451@163.com。

更新日期/Last Update: 2014-08-15