ZHAO Yu-ming,JIANG Jun,ZHANG Jian-ning,et al.Investigation on Rapid Measurement of Water-soluble Soybean Protein through Spectrophotometry[J].Soybean Science,2010,29(01):124-126.[doi:10.11861/j.issn.1000-9841.2010.01.0124]
光度法快速测定大豆水溶性蛋白质的研究
- Title:
- Investigation on Rapid Measurement of Water-soluble Soybean Protein through Spectrophotometry
- 文章编号:
- 1000-9841（2010）01-0124-03
- Keywords:
- Protein; Azocarmine G; Water-soluble protein; Spectrophotometry
- 分类号:
- TS210.7
- 文献标志码:
- A
- 摘要:
- 在室温条件下的酸性介质中，大豆水溶性蛋白质能与偶氮洋红G（azocarmine G）迅速结合，生成紫红色复合物，该复合物的最大吸收波长为580 nm，比偶氮洋红G红移了50 nm，其吸光强度与大豆水溶性蛋白质的质量浓度在3～34 μ·mL-1范围内存在较好的选择性和较宽的线性关系。依此建立了测定大豆水溶性蛋白质的新方法，同时优化了大豆水溶性蛋白质的提取过程。该方法具有快速、稳定的特点，与凯氏法所得测定结果基本一致。
- Abstract:
- A purple-red complex could be formed rapidly between azocarmine G and water-soluble soybean protein in acidic medium.The absorbance of this complex had a maximum absorption wavelength at 580 nm and exhibited a bathochromic shift of 50 nm compared with the maximum absorption wavelength of azocarmine G.Its absorbance with the concentration of soluble proteins at least in 3~34 μ·mL-1 was linearly.A method on rapid measurement of water-soluble soybean protein was established based on above-mentioned.Simultaneously we optimized the extraction procedure of water-soluble soybean protein.The results obtained by this method was rapid，stable.And the results were same with that obtained by Kjeldahl’s method.
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备注/Memo
第一作者简介：赵宇明（1977-），男，工程师，硕士，现从事食品检测与方法研究。E-mail:zhao_yuming@126.com。