[1]宋志峰,王丽,孟凡钢,等.吉林省普通大豆品种（系）异黄酮含量分析[J].大豆科学,2009,28(06):1076-1080.[doi:10.11861/j.issn.1000-9841.2009.06.1076]
　SONG Zhi-feng,WANG Li,MENG Fan-gang,et al.Analyse of Isoflavones Content in Normal Soybean Varieties（Lines）in Jilin Province[J].Soybean Science,2009,28(06):1076-1080.[doi:10.11861/j.issn.1000-9841.2009.06.1076]
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吉林省普通大豆品种（系）异黄酮含量分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1076-1080 栏目: 出版日期: 2009-12-25
Title:
Analyse of Isoflavones Content in Normal Soybean Varieties（Lines）in Jilin Province
文章编号:
1000-9841(2009)06-1076-05
作者:
宋志峰1; 王丽1; 孟凡钢2; 王新风2; 黄璜1; 马巍2; 富健2
1.吉林省农业科学院 农业环境与资源研究中心，吉林 长春 130033；
2.吉林省农业科学院 大豆研究中心，吉林 长春 130033
Author(s):
SONG Zhi-feng1; WANG Li1; MENG Fan-gang2; WANG Xin-feng2; HUANG Huang1; MA Wei2; FU Jian2
1.Research Center of Agricultural Enviroment and Resource，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin；
2.Research Center of Soybean，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin，China
关键词:
HPLC; 大豆籽粒; 异黄酮; 测定
Keywords:
HPLC;  Soybean;  Isoflavone;  Determination
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1076
文献标志码:
A
摘要:
利用超声波法对吉林省26份普通大豆品种（系）大豆异黄酮含量进行HPLC测定。建立了一种HPLC测定大豆籽粒中异黄酮含量的方法。结果表明：异黄酮各异构体在50~1000 μmol.L-1范围内线性系数良好，相关系数为0.9998~0.9999；检出限为1.09~2.06 mg.L-1，定量限为3.25~6.46 mg.L-1；异黄酮各异构体回收率为97.11%~103.31%；相对标准偏差（RSD，n=7）分别为2.03%~4.32%。该方法线性范围广，线性关系好，灵敏度和准确度高，适合于大豆及大豆制品中异黄酮含量的测定。测得吉林省261份普通大豆品种（系）中的异黄酮含量范围1.46～4.97 mg.g-1，超过4 mg.g-1的品种（系）有23个，占测定品种（系）总数的8.81%。
Abstract:
A HPLC method for rapid determination of isoflavones from soybean is presented.The isoflavones were extracted by 80% methanol and followed hydrolyzing at 100℃ to complete conversion of the acetyl and malonyl forms of isoflavones to β-glucoside and aglucone forms.The isoflavones were separated on a C18 short column using gradient elution；column oven temperature，40℃；the diode array detector monitoring wavelength ，260 nm.The peak area of isoflavones versus their concentration had a good linearity（correlation coefficient were 0.9998-0.9999）in the range of 50-1000 μmol.L-1.Limits of detection(LOD）were in the range of 1.09-2.06 mg.L-1，limits of quantification(LOQ）were in the range of 3.25-6.46 mg.L-1.The recoveries of standard spiking of isoflavones were in the range of 97.11%-103.31%.The relative standard deviation(RSD，n=7）were in the range of 2.03%-4.32%.The linearity of the method was good in a wide range.This method has high analytic sensitivity and accuracy,and was suitable for determination of isoflavones in soybean and other cereal products.The isoflavone content in 261 Jilin province normal soybean varieties（lines）ranged from 1.46 mg.g-1.to 4.97 mg.g-1.The isoflavone content of 23 varieties（lines）soybean exceeded 4 mg.g-1.The research results indicated that the content range and characteristics of the soybean oligosaccharides in Jilin province and lay a foundation for the further research of soybean quality breeding and processing and utilization of soybean isoflavone.

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备注/Memo

基金项目：吉林省农业科学院大豆研究中心青年基金资助项目。

第一作者简介：宋志峰（1975-）,男,助理研究员,研究方向为农产品质量与安全。
通讯作者：富健，研究员。 E-mail：tywt0001@yahoo.com.cn。

更新日期/Last Update: 2014-09-12