[1]于安亮,徐鹏飞,陈晨,等.大豆疫霉根腐病子叶接种法抗病性鉴定[J].大豆科学,2009,28(05):879-882，888.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
　YU An-liang,XU Peng-fei,CHEN Chen,et al.Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method[J].Soybean Science,2009,28(05):879-882，888.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
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大豆疫霉根腐病子叶接种法抗病性鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 879-882，888 栏目: 出版日期: 2009-10-25
Title:
Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method
文章编号:
1000-9841(2009)05-0883-06
作者:
于安亮1; 徐鹏飞1; 陈晨1; 王金生1; 吴俊江2; 李宁辉1; 李文滨1; 马凤鸣3; 邱丽娟4; 常汝镇4; 陈维元5; 张淑珍1
1.东北农业大学 大豆研究所，教育部大豆生物学重点实验室，黑龙江 哈尔滨，150030；
2.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨，150086；
3.东北农业大学 农学院，黑龙江 哈尔滨 150030；
4.中国农业科学院 作物科学研究所，北京100081；
5.黑龙江省农业科学院 绥化分院，黑龙江 绥化 152052
Author(s):
YU An-liang1; XU Peng-fei1; CHEN Chen1; WANG Jin-sheng1; WU Jun-jiang2; LI Ning-hui1; LI Wen-bin1; MA Feng-ming3; QIU Li-juan4; CHANG Ru-zhen4; CHEN Wei-yuan5; ZHANG Shu-zhen1
1.Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology of Chinese Education Ministry，Harbin 150030，Heilongjiang；2.Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；3.College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang；
关键词:
抗病性鉴定; 子叶接种法; 下胚轴接种法; 大豆疫霉根腐病
Keywords:
4.Crop Sciences Institute; Chinese Academy of Agricultural Sciences; Beijing 100081; Disease resistance identification; Cotyledon inoculation; Hypocotyl inoculation; Phytophthora sojae
分类号:
S435.651
DOI:
10.11861/j.issn.1000-9841.2009.05.0879
文献标志码:
A
摘要:
用子叶接种法鉴定了来自黑龙江、内蒙古、湖北、以及四川的65个大豆品种对疫霉根腐病1号生理小种的抗感情况。其中抗病品种11个，中间类型5个，感病品种49个。其鉴定结果与下胚轴接种法比较相同的有51个品种。经过DPS v7.05分析下胚轴接种法与子叶接种法之间的相关呈极显著，经r×c独立性测验得到χ2=1.46<χ0.05，22=5.99，2种方法差异不显著。证明了子叶接种法进行大豆疫霉根腐病抗性鉴定同样准确可行。此外还可以解决遗传分析上需要保存感病植株后代的问题，为遗传分析时鉴定疫霉根腐病抗感情况提供了可靠的方法。
Abstract:
Soybean production is seriously affected by Phytophthora sojae，one kind of destructive fungal diseases.The most effective measure to prevent and control the diseases is to carry out disease-resistant breeding. In this paper，65 soybean varieties from Heilongjiang，Inner Mongolia，Hunan and Sichuan were evaluated the reactions to P.sojae race 1 by way of cotyledon inoculation.Of all the varieties tested，11 were resistant，49 were susceptible，and the rest were intermediate. Fifty-one varieties had the same reaction as those evaluated with hypocotyl inoculation method.There was significant correlation between cotyledon inoculation and hypocotyl inoculation method according to DPS V7.05 analysis.By the independent tests of r×c table of cotyledon inoculation and vaccination hypocotyl，there were no significant differences between the two methods.Cotyledon inoculation was proved to be feasible to evaluate soybean germplasm infection with P.sojae compared with the commonly used hypocotyl inoculation method.It not only provided a method to identify the soybean resistance to P.sojae，but also solved the problem that the seeds of susceptible plants generated from crossing progeny could not be kept using hypocotyl inoculation method.

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备注/Memo

基金项目：国家自然科学基金资助项目(30671317, 30810103063, 30400285)；中国博士后基金资助项目(20060400835)；黑龙江省自然科学基金资助项目（C200814）；国家重点基础研究发展计划资助项目（2004CB117203-4）；黑龙江省新世纪人才培养计划资助项目(NCET-06-007)；黑龙江省农科院博士后基金资助项目（LRB06-010）；农业部寒地作物重点开放实验室开放课题资助项目。

作者简介：于安亮（1983-），男，硕士研究生，研究方向为大豆抗病遗传育种。
通讯作者：张淑珍，博士，教授。 E-mail:dnzhshzh@yahoo.com.cn。

更新日期/Last Update: 2014-09-18