[1]尹俊琦,王 楠,周 莹,等.两个大豆品种转hrpZPsta基因后代对大豆灰斑病的抗性分析[J].大豆科学,2013,32(02):238-241.[doi:10.3969/j.issn.1000-9841.2013.02.022]
　YIN Jun-qi,WANG Nan,ZHOU Ying,et al.Analysis on Resistance to Frogeye Leaf Spot between Two Species of Transgenic Soybean Progenies with HrpZpsta Gene[J].Soybean Science,2013,32(02):238-241.[doi:10.3969/j.issn.1000-9841.2013.02.022]
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两个大豆品种转hrpZPsta基因后代对大豆灰斑病的抗性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年02期 页码: 238-241 栏目: 出版日期: 2013-04-25
Title:
Analysis on Resistance to Frogeye Leaf Spot between Two Species of Transgenic Soybean Progenies with HrpZpsta Gene
作者:
尹俊琦; 王 楠; 周 莹; 卢 实; 吴 楠; 曲 静; 张 卓; 王丕武
（吉林农业大学 农学院，吉林 长春 130118）
Author(s):
YIN Jun-qi; WANG Nan; ZHOU Ying; LU Shi; WU Nan; QU Jing; ZHANG Zhuo; WANG Pi-wu
（College of Agronomy,Jilin Agricultural University，Changchun 130118,China）
关键词:
大豆灰斑病; hrpZpsta基因; 抗病性鉴定; 抗性评价
Keywords:
Soybean Cercospora sojina; hrpZpsta gene; Screening for disease resistance; Evaluation of resistance
DOI:
10.3969/j.issn.1000-9841.2013.02.022
文献标志码:
A
摘要:
大豆灰斑病是由真菌类病原菌引发的，能导致大豆植物体整株死亡的恶性植物病害。hrpZpsta基因所编码的激发子harpin蛋白，已被证实能够通过引起植物过敏性反应来提高植株机体对多种病原菌的抗性。将含有hrpZpsta基因的植物表达载体转化到大豆品种吉农17和吉农29中，以所得的T4代株系为试验材料，通过分子生物学方法与人工接菌方式对其进行检测。Southern blot和RT-PCR鉴定结果表明hrpZpsta基因能够在吉农17和吉农29的T4代转化株系中稳定的遗传和表达，并且转化植物后代对灰斑病病原菌的抗性较受体品种明显提高。此外，不同大豆受体品种对灰斑病的抗性存在差异。
Abstract:
Soybean Cercospora sojina is caused by fungal pathogens,which lead to plant death.Protein harpin which coded by gene hrpZpsta has been proved to elevate resistance for plants against several kinds of fungal pathogen by hypersensitive reaction.The recombinant expressed vector which contained hrpZpsta gene had been transformed into two soybean cultivars,Jinong 17 and Jinong 29,and T4 transgenic plants were identified both in molecular way and artificial inoculations.Southern blot and RTPCR test showed that hrpZpsta gene could inherit in T4 transgenic plants steadily on the molecular level,and artificial inoculation test revealed the resistant ability to Cercospora sojina had been improved obviously.In addition,the resistance to frogeye leaf spot varied with soybean cultivars.

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备注/Memo

?转基因生物新品种培育重大专项（2011ZX08004-004）。

更新日期/Last Update: 2014-04-03