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[1]白丽,李海朝,马莹,等.大豆对大豆花叶病毒SC-11株系抗性的遗传及基因定位[J].大豆科学,2009,28(01):1-6.[doi:10.11861/j.issn.1000-9841.2009.01.0001]
　BAI Li,LI Hai-chao,MA Ying,et al.Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC-11 in Soybean[J].Soybean Science,2009,28(01):1-6.[doi:10.11861/j.issn.1000-9841.2009.01.0001]

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大豆对大豆花叶病毒SC-11株系抗性的遗传及基因定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷期数: 2009年01期页码: 1-6 栏目: 出版日期: 2009-02-25

Title:: Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC-11 in Soybean

文章编号:: 1000-9841(2009)01-0001-06

作者:: 白丽; 李海朝; 马莹; 王大刚; 刘宁; 智海剑; 南京农业大学国家大豆改良中心，作物遗传与种质创新国家重点实验室，江苏南京 210095

Author(s):: BAI Li; LI Hai-chao; MA Ying; WANG Da-gang; LIU Ning; ZHI Hai-jian; National Center for Soybean Improvement，National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University，Nanjing 210095，Jiangsu，China

关键词:: 大豆花叶病毒; 抗性遗传; 等位性; 基因定位; 分子遗传连锁图

Keywords:: Soybean mosaic virus（SMV); Resistance inheritance; Allelism; Gene mapping; Molecular genetic linkage map

分类号:: S565.1

DOI:: 10.11861/j.issn.1000-9841.2009.01.0001

文献标志码:: A

摘要:: 大豆花叶病毒病（soybean mosaic virus，SMV）是世界性大豆病害，严重危害大豆的产量和质量。SC-11为我国黄淮夏大豆区以及北方春大豆区SMV主要流行株系。研究大豆品种对SC-11的抗性遗传方式，不同大豆材料对SC-11抗性位点的等位性关系，并对抗性基因进行了SSR标记定位。结果表明：齐黄1号对SC-11的抗性由一对显性基因（R_SC-11）控制；齐黄1号、齐黄22、广吉和早熟18对SC-11的抗病基因是等位的或紧密连锁的；经分离群体组群分析法研究发现，齐黄1号抗SC-11的位点R_SC-11位于F连锁群，与SSR标记Satt114、Satt334、Sat_234和Sct_033紧密连锁，距离分别为11.1 cM、8.9 cM、4.6 cM和4.7 cM；选取F连锁群上亲本间有多态的18对引物构建了F连锁群的遗传图谱，全长254.8cM，标记间平均距离为13.41cM。研究结果为大豆抗病育种以及抗性基因的精细定位和图位克隆奠定了基础。

Abstract:: Soybean mosaic virus（SMV）is one of major viral diseases in soybean production worldwide.It causes yield loss and seed quality deficiency seriously.SMV strain SC-11 is a common prevalent strain in both of the Huang-Huai summer planting soybean region and the northern China spring planting soybean region.The major objectives of this research were to determine the inheritance of resistance，test the allelism of the resistance genes among the resistant cultivars，map the resistant gene to SC-11.The results showed that Qihuang No.1 has a single dominant resistance gene to SC-11.The resistance genes to SC-11 among Qihuang No.1，Qihuang 22，Kwanggyo and Zaoshu 18 were allelic.Bulk segregant analysis(BSA）demonstrated that in the F-2 mapping population from Qihuang No.1×Nannong1138-2，Four SSR markers Satt114，Satt334，Sat_234 and Sct_033 in F linkage group were found closely linked to R_SC-11，with genetic distances of 11.1cM，8.9 cM，4.6 cM and 4.7 cM，respectively.R_SC-11was located between Sat_234 and Sct_033 in F linkage group.Using 18 SSR markers locating in F linkage group of the integrated joint map and expressing polymorphism between the two parents，the genetic map of the F linkage group was constructed.The full length of the linkage group is 245.8cM with an average interval of 13.41cM between two markers.

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备注/Memo

基金项目：国家自然科学基金资助项目（30571176）；国家科技支撑计划资助项目（2006BAD01A04）；国家高技术研究发展计划（863计划）资助项目（2006A10A111）；高等学校创新引智计划资助项目（Supported by the 111 project）(B08025)。

作者简介：白丽（1982-），女，硕士研究生，现从事大豆抗病遗传育种研究。

更新日期/Last Update: 2014-09-29