[1]夏循礼,杨广笑,何光源.大豆异黄酮生物合成串联基因簇及其表达载体的构建[J].大豆科学,2009,28(01):7-10,20.[doi:10.11861/j.issn.1000-9841.2009.01.0007]
　XIA Xun-li,YANG Guang-xiao,HE Guang-yuan.Design of Tandem Genes Cluster and Expression Vectors for Soybean Isoflavone Biosynthesis[J].Soybean Science,2009,28(01):7-10,20.[doi:10.11861/j.issn.1000-9841.2009.01.0007]
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大豆异黄酮生物合成串联基因簇及其表达载体的构建

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 7-10,20 栏目: 出版日期: 2009-02-25
Title:
Design of Tandem Genes Cluster and Expression Vectors for Soybean Isoflavone Biosynthesis
文章编号:
1000-9841(2009)01-0007-04
作者:
夏循礼; 杨广笑; 何光源
华中科技大学中英HUST-RRes基因工程和基因组学联合实验室，湖北 武汉 430074
Author(s):
XIA Xun-li; YANG Guang-xiao; HE Guang-yuan
China UK HUST RRes Genetics Engineering and Genomics Joint Laboratory，Huazhong University of Science and Technology，Wuhan 430074，Hubei，China
关键词:
大豆异黄酮; 生物合成; 串联基因簇; 表达载体
Keywords:
Soybean isoflavones; Biosynthesis; Tandem genes cluster; Expression vector
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0007
文献标志码:
A
摘要:
植物异黄酮是一种重要的次级代谢产物，在植物的生理活动以及人类健康方面具有重要作用。植物异黄酮生物合成途径有三个调控酶：查尔酮合酶(Chalcone synthase，CHS)，查尔酮异构酶(Chalcone Isomerase，CHI)和异黄酮合酶(Isoflavone synthase，IFS)。以大豆为材料，利用RT-PCR方法克隆到查尔酮合酶基因(CHS)，查尔酮异构酶基因(CHI Ⅱ)和异黄酮合酶基因(IFS)的cDNA（Genebank登记号分别为EU526827，EU526829，EU526830）经过NCBI在线Blast比对，相似度均在98％以上，确认克隆的目标基因是正确的；将这三种基因顺序连接，构建成串联基因簇CHS- CHI ⅡIFS（命名为rIFS)，并且构建了rIFS的原核表达载体，进行了初步的表达研究。为进一步研究植物异黄酮的生物合成以及进行异黄酮生物工程奠定了基础。
Abstract:
Plant isoflavones is a sort of important secondary metabolites with signification in plant physiological function and healthiness for human being.There are three regulating enzymes in plant isoflavones biosynthesis pathway：Chalcone synthase(CHS)，Chalcone isomerase(CHI）and Isoflavone synthase(IFS).In this article，we cloned the cDNAs of CHS，CHIⅡ，and IFS using RT-PCR method；then assembled these three genes to make a tandem genes cluster-CHS-CHI Ⅱ-IFS(we named it as rIFS)；finally we constructed the E coli.expression vector of pET-rIFS and researched its expression.All these made the groundwork for further research on soybean isoflavone biosynthesis and isoflavone engineering.

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备注/Memo

基金项目：国家重点基础研究发展计划（973计划）前期专项资助项目（2005CCA1900）。

作者简介：夏循礼（1969-），男，博士研究生，研究方向为药用植物的分子生物学与生物技术研究。E-mail:xiaxunli@126.com。

更新日期/Last Update: 2014-09-29