[1]李铁,刘昭军,刘丽艳,等.一种野生大豆根际土壤细菌的分离及其防治大豆病害的研究[J].大豆科学,2008,27(06):1007-1009,1014.[doi:10.11861/j.issn.1000-9841.2008.06.1007]
　LI Tie,LIU Zhao-jun,LIU Li-yan,et al.Isolation of a Bacteria from Wild Soybean Rhizosphere Soil and Its Function to Control Soybean Diseases[J].Soybean Science,2008,27(06):1007-1009,1014.[doi:10.11861/j.issn.1000-9841.2008.06.1007]
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一种野生大豆根际土壤细菌的分离及其防治大豆病害的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 1007-1009,1014 栏目: 出版日期: 2008-12-25
Title:
Isolation of a Bacteria from Wild Soybean Rhizosphere Soil and Its Function to Control Soybean Diseases
文章编号:
1000-9841(2008)06-1007-03
作者:
李铁1; 刘昭军1; 刘丽艳1; 张莉莉1; 成瑜1; 马启慧2
1黑龙江省农业科学院生物技术研究所，黑龙江 哈尔滨 150086；
2黑龙江省农业科学院信息中心，黑龙江 哈尔滨 150086
Author(s):
LI Tie1; LIU Zhao-jun1; LIU Li-yan1; ZHANG Li-li1; CHENG Yu1; MA Qi-hui2
1.Biotechnology Research Institute，Heilongjiang Academy of Agricultural Sciences(HAAS)，Harbin 150086,Heilongjiang；
2.Infornation Institute of HAAS，Harbin 150086，Heilongjiang，China
关键词:
野生大豆; 拮抗; 大豆连作
Keywords:
Glycine soja; Antagonism; Soybean continuous cropping
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.1007
文献标志码:
A
摘要:
为筛选拮抗大豆根腐病的土壤微生物，以邻苯二甲酸为唯一碳源培养基，从野生大豆根际土壤中分离获得1株细菌，命名为HK26-21。通过平皿接种以及16s rDNA序列扩增，根据其生理生化特征和16s rDNA（GenBank Accession No.EF032879）序列相似性分析，将该菌株鉴定为芽孢杆菌属(Bacillus)。通过单因素试验法确定了最佳降解条件：温度为25 ℃，pH为7.0，降解速率与接种量呈正相关。平皿微生物对峙试验表明菌株可拮抗大豆菌尖孢镰刀菌和立枯丝核菌。初步认为所分离野生大豆根际芽孢杆菌HK26-21可以缓解大豆连作障碍。
Abstract:
Through screening edaphon resisting soybean root rot，one bacterial strain was isolated from wild soybean rhizosphere in medium with phthalate as only carbonaceous organic compound.It could digest phthalate and was named HK26-21.Through plate inoculation and 16s rDNA sequence amplification，it was showed that HK26-21 was Bacillus according to physiology-biochemistry characteristics and the similarity of 16s rDNA analysis.Through single factor test，the optimal digesting conditions were：temperature 25 ℃，pH 7.0.Digesting rate had positive correlation with the amount of inoculums.Test of colony standoff in plate proved the bacterium could resist soybean pathogenic fungi(Fusarium oxysporum and Rhizoctonia solani).It was considered that the isolated bacterium from wild soybean rhizosphere could have biological control efficacy against pathogens of soybean root rot.

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备注/Memo

基金项目：黑龙江省农业科学院青年基金资助项目（2006年度）。

作者简介：李铁（1979-），男，研究实习员，主要从事作物基因工程及分子育种。

更新日期/Last Update: 2014-10-06