[1]卢双勇,韩英鹏,滕卫丽,等.大豆抗花叶病毒及耐疫霉根腐病的SSR标记分析[J].大豆科学,2008,27(05):746-750.[doi:10.11861/j.issn.1000-9841.2008.05.0746]
　LU Shuang-yong,HANG Ying-peng,TENG Wei-li,et al.Molecular Markers Associated with Soybean Mosaic Virus Resistance and Phytophthora Root Rot Tolerance in Soybean[J].Soybean Science,2008,27(05):746-750.[doi:10.11861/j.issn.1000-9841.2008.05.0746]
点击复制

大豆抗花叶病毒及耐疫霉根腐病的SSR标记分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 746-750 栏目: 出版日期: 2008-10-25
Title:
Molecular Markers Associated with Soybean Mosaic Virus Resistance and Phytophthora Root Rot Tolerance in Soybean
文章编号:
1000-9841(2008)05-0746-05
作者:
卢双勇; 韩英鹏; 滕卫丽; 李文滨
东北农业大学大豆研究所，国家教育部大豆生物学重点实验室，黑龙江 哈尔滨 150030
Author(s):
LU Shuang-yong; HANG Ying-peng; TENG Wei-li; LI Wen-bin
Soybean Research Institate,Northeast Agricultural University，Key Laborary of Soybean Biology of Chinese Education Ministry,Harbin 150030,Heilongjiang,China
关键词:
大豆花叶病毒; 大豆疫霉根腐病; SSR标记
Keywords:
Soybean mosaic virus(SMV); Soybean phytophthora root rot(PRR); SSR marker
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0746
文献标志码:
A
摘要:
抗大豆花叶病毒而感大豆疫霉根腐病亲本东农93-046与感大豆花叶病毒耐大豆疫霉根腐病亲本Conrad及其杂交所得的160个F5 RIL群体分别接种SMV N1、SMV N3株系及从佳木斯田间分离得到的疫霉根腐病混合菌种，结果表明：东农93-046对花叶病毒表现为抗病而对疫霉根腐病表现为感病，Conrad则相对应的表现为感病和耐病；RIL群体中对花叶病毒SMV N1、SMV N3的抗感表现都按1:1分离（χ2= 0.64*，χ2 = 0.43*），疫霉根腐病病害损失率基本符合正态分布（skew=0.93，kurtosis= 0.86）。利用SSR分子标记技术对该群体进行花叶病毒病抗性基因定位和疫霉根腐病的耐性QTL分析，分别将花叶病毒抗性基因RN1、RN3定位于F连锁群，其与Satt114的距离分别为5.2 cM 和4.9 cM，同时在该连锁群检测到一个与疫霉根腐病耐病性相关的QTL 即QPR_1，其与Satt252的距离为4.5 cM，在D1b+W连锁群上检测到两个与疫霉根腐病耐病性相关的QTL即是QPR_2、QPR_3，其与Satt428（Satt579）、Satt274的距离分别为1.05 cM，2.00 cM 。
Abstract:
The purpose of this study is to identify molecular loci resistance to soybean mosaic virus and to identify quantitative trait loci (QTLs) associated with the tolerance to soybean phytophthora root rot (PRR) using 160 F5?derived RI lines from a combination between Dongnong 93-046 (a soybean cultivar resistant to SMV but susceptible to PRR) and Conrad (a breeding line susceptible to SMV but tolerance to PRR).The results showed that the resistant and susceptible segregation in RIL population was fitted to ratio 1：1 for SMV，and the loss rate from PRR inoculation fit to normal distribution.Two SMV resistant loci and three QTLs associated with PRR tolerance were identified using SSR technique.SMV Resistant loci，RN1 and RN3 was located in MLGF and tightly linked with SSR makers Satt114 and Satt362.QTL QPR_1 associated with PRR tolerance was also be located in MLG F.QTLs QPR_2 and QPR_3 was located in MLGD1b+W.The phenotypic contribution of the three QTLs were 9.38%，19.4% and 7.02%，respectively.

参考文献/References:

[1]Kiihl R A S，Hartwig E E.Inheritance of reaction to soybean mosaic-virus in soybeans[J].Crop Science，1979，19:372-375.

[2] Buzzell R I，Tu J C.Inheritance of soybean resistance to soybean mosaic virus[J].Heredity，1984，75 (1):82.
[3]Buzzell R I，Tu J C.Inheritance of a soybean stem-tip necrosis reaction to soybean mosaic virus[J].Heredity，1989，80(5):400-401.
[4] Ma G，Chen P，Buss G R，et al.Genetic characteristics of two genes for resistance to soybean mosaic virus in PI486355 soybean[J].Theoretical and Appled Genetics，1995，91(6):907-914.
[5]盖钧镒.我国大豆遗传改良和种质研究[M]//宋健.中国科学技术前沿，第五卷，北京:高等教育出版社，2002: 629-691.(Gai J Y.Genetic improvement and germplasm study of soybean in China[M]//Song J.Science and Technology at the Frontier in China，Vol.5，Beijing:Higher Education Press，2002: 629-691.)
[6]张玉东，盖钧镒，马育华，等.大豆对两个大豆花叶病毒本地株系抗性的遗传研究[J].作物学报，1989，15(3):213-220.(Zhang Y D，Gai J Y，Ma Y H，et al.Inheritance of resistance to two local soybean mosaic virus strains S_A and S_C in soybeans[J].Acta Agronomica Sinica，1989，15(3):213-220.)
[7]向远道，盖钧镒，马育华，等.大豆对四个大豆花叶病毒株系的抗性及其连锁遗传研究[J].遗传学报，1991，18 (1):51-58.(Xiang Y D，Gai J Y，Ma Y H，et al.A study of the inheritance and linkage of resistance to four strains of soybean mosaic virus in soybeans[J].Acta Genetica Sinica，1991，18 (1): 51-58.)
[8]孙志强，刘玉芝，孙大敏，等.大豆对大豆花叶病毒1、2、3号毒系抗性的遗传[J].中国油料作物学报，1990(2):20-24.(Sun Z Q，LiuY Z，Sun D M，et al.Inheritance of resistance to SMV strain 1.2.3 in soybeans[J].Chinese Journal of Oil Crop Sciences，1990 (2):20-24.)
[9]廖林，刘玉芝，孙大敏，等.大豆花叶病的抗性遗传Ⅰ.几个引用抗原对东北大豆花叶病毒二号株系的抗性遗传[J].遗传学报，1994，21(5):403-408.(Liao L，LiuY Z，Sun D M，et al.Inheritance of soybean resistant to soybean mosaic virus Ⅰ.Inheritance of several resistant varieties introduced resistant to No.Ⅱ strain of SMV[J].Acta Genetica Sinica，1994，21(5):403-408.)
[10]栾晓燕.大豆对SMV3号株系成株抗性遗传的研究[J].大豆科学，1997，16(3):223-226.(Luan X Y.Inhereitance of soybean plant resistant to No.3(173)strain of soybean mosaic virus[J].Soybean Science，1997，16(3):223-226.)
[11]智海剑，盖钧镒.大豆花叶病毒及抗性遗传的研究进展[J].大豆科学，2006，25(2):174-180.(Zhi H J，Gai J Y.Advance in the study on soybean mosaic virus[J].Soybean Science，2006，25(2):174-180.)
[12] Wrather J A，Anderson T R，Arsyad D M，et al.Soybean disease loss estimates for the top ten soybean-producing countries in 1998[J].Canadian Journal of Plant Pathology，2001，23: 115-121.
[13]韩晓增，何志鸿，张增敏.大豆主要病虫害防治技术[J].大豆通报，1998，6:5-6.(Han X Z，He Z H，Zhang Z M.Main plant diseases and insects pests prevention and cure technique in soybean[J].Soybean Bulletin，1998(6):5-6.)
[14]朱振东，王化波，王晓鸣，等.中国大豆疫霉菌分布及毒力多样性研究[J].中国农业科学，2003，36:793-799.(Zhu Z D，Wang H B，Wang X M，et al.Distribution and virulence diversity of Phytophthora sojae in China[J].Scientia Agricultura Sinica，2003，36:793-799.)
[15]Gordona S G，Martin S K St，Dorrancea A E.Rps8 maps to resistance gene rich region on soybean molecular linkage group F[J].Crop Science，2006，46: 168-173.
[16] Schafer J F.Tolerance to plantdisease.[J].Annual Review of Phytopathology，1971，9:235-252.
[17]Thomison P R，Thomas W J Kenworth.Evidence of pathogen specify in tolerance of soybean cultivars to phytophthora rot[J].Crop Science，1988，28:714-15.
[18]滕卫丽，李文滨，邱丽娟，等.大豆SMV 3号株系抗病基因的SSR标记[J].大豆科学，2005(3):244-249.(Teng W L，Li W B，Qiu L J，et al.Identification of SSR marker linked to the resistance gene of SMV3 in soybean[J].Soybean Science，2005(3):244-249.)
[19]Han Y P，Teng W L，Yu K F，et al.Mapping QTL tolerance to Phytophthora root rot in soybean using microsatellite and RAPD/SCAR derived markers[J].Euphytica，2008，162:231-239.
[20]王珍，方宣钧.植物DNA分离[J].分子植物育种，2003，1(2):281-288.(Wang Z，Fang X J.Plant DNA isolation[J].Molecular Plant Breeding，2003，1(2):281-288.)
[21]Lander E S，Green P，Abrahamson J，et al.Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations[J].Genomics，1987，1:174-181.
[22]Koshimizu S，Lizuka T.Studies on soybean virus diseases in Japan[C].Natl.Agric.Exp.Stn.Bull.1963，27，1-103.
[23]Hayes A J，Ma G，Buss G R，et al.Molecular marker mapping of Rsv4，a gene conferring resistance to allknown strains of soybean mosaic virus[J].Crop Science，2000，40:1434-1437.
[24]王永军，东方阳，王修强，等.大豆5个大豆花叶病毒株系抗性基因的定位，遗传学报，2004，31(1):87-90.(Wang Y J，Dongfang Y，Wang X Q，et al.Mapping of five genes resistant to SMV strains in soybean[J].Acta Genetica Sinica，2004，31(1):87-90.)

相似文献/References:

[1]李 凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
　LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
[2]韩英鹏,程 章,赵 雪,等.大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定[J].大豆科学,2013,32(06):740.[doi:10.11861/j.issn.1000-9841.2013.06.0740]
[3]高 乐,宋英培,李 凯,等.大豆花叶病毒HC-Pro基因保守序列克隆及其RNAi载体的构建[J].大豆科学,2013,32(06):744.[doi:10.11861/j.issn.1000-9841.2013.06.0744]
[4]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(05):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[5]王大刚,田 震,李 凯,等.鲁豫皖大豆产区大豆花叶病毒株系的鉴定及动态变化分析[J].大豆科学,2013,32(06):806.[doi:10.11861/j.issn.1000-9841.2013.06.0806]
[6]张雯娜,李晋玉,田金艳,等.逆转录环介导等温扩增技术快速检测大豆花叶病毒[J].大豆科学,2014,33(03):422.[doi:10.11861/j.issn.10009841.2014.03.0422]
　ZHANG Wen-na,LI Jin-yu,TIAN Jin-yan,et al.Rapid Detection of Soybean Mosaic Virus by Reverse Transcription Loop Mediated Isothermal Amplification[J].Soybean Science,2014,33(05):422.[doi:10.11861/j.issn.10009841.2014.03.0422]
[7]韩英鹏,赵 雪,李修平,等.大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定[J].大豆科学,2014,33(01):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
　HAN Yingpeng,ZHAO Xue,LI Xiuping,et al.SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot[J].Soybean Science,2014,33(05):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
[8]杨永庆,侯文焕,边全楽,等.河北地区大豆花叶病毒株系的组成与分布[J].大豆科学,2014,33(01):87.[doi:10.11861/j.issn.1000-9841.2014.01.0087]
　YANG Yong-qing,HOU Wen-huan,BIAN Quan-le,et al.Composition and Distribution of SMV Strains in Hebei[J].Soybean Science,2014,33(05):87.[doi:10.11861/j.issn.1000-9841.2014.01.0087]
[9]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(06):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
　SHEN Hong-bo,YAO Wen-qiu,YU Yong-mei,et al.Control Efficiency of Different Biopesticides on Soybean Phytophthora Root Rot[J].Soybean Science,2011,30(05):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
[10]王大刚,张 磊,智海剑.大豆花叶病毒株系鉴定与分子生物学研究进展[J].大豆科学,2012,31(04):668.[doi:10.3969/j.issn.1000-9841.2012.04.031]
　WANG Da-gang,ZHANG Lei,ZHI Hai-jian.Advances in Identification of Strains and Molecular Biology of Soybean Mosaic Virus[J].Soybean Science,2012,31(05):668.[doi:10.3969/j.issn.1000-9841.2012.04.031]

备注/Memo

基金项目：国家高技术研究发展计划（863计划）资助项目（2006AA10Z1F1）。

作者简介：卢双勇（1982-），男，硕士研究生，研究方向大豆生物技术、大豆抗病育种。
通讯作者：李文滨，教授，博士生导师。E-mail:wenbinli@yahoo.com。

更新日期/Last Update: 2014-10-06