LIU Ming,TAN Bin,WU Yong-pei,et al.Optimization of Condition for Liquid State Fermenting Soybean Antioxidative Peptide[J].Soybean Science,2008,27(03):509-514.[doi:10.11861/j.issn.1000-9841.2008.03.0509]
液态发酵大豆抗氧化活性肽条件的优化
- Title:
- Optimization of Condition for Liquid State Fermenting Soybean Antioxidative Peptide
- 文章编号:
- 1000-9841(2008)03-0509-06
- Keywords:
- Liquid state fermentation; Soybean antioxidative peptide; Antioxidant activity; Response surface methodology
- 分类号:
- Q815.S529.TQ936.1+6
- 文献标志码:
- A
- 摘要:
- 筛选和优化液态发酵法生产大豆抗氧化活性肽的条件。以总抗氧化活性的强弱为指标,通过对发酵温度、种龄、pH值、接种量、料液比、发酵时间等条件作单因素、析因试验确定主要影响因素,并采用最速上升试验和中心组合二次旋转回归响应面法优化pH值、接种量。优化获得液态发酵大豆抗氧化活性肽的最佳工艺参数为发酵温度37℃,种龄36 h、pH值为7.39,接种量为5.19%,料液比4%,发酵时间36 h。在此条件下发酵产物总抗氧化活性795.75 U.g-1(豆粕),试验值与预测值基本相符。
- Abstract:
- In order to optimize the liquid state fermenting conditions of soybean antioxidative peptide,the single factor experiments and factorial experiment design for medium rate,fermentation temperature,inoculative rate,primary pH value and water addition were carried out using total antioxidant activity as index.Then the reactive temperature and pH were optimized by steepest ascent design and secondary regression common rotary combination design.Results showed that suitable technologcial conditions of liquid state fermentation for soybean antioxidative peptide were: the medium rate 4%,inoculative rate 5.19%,age of inoculum 36 h,fermentation temperature 37.0℃,primary pH value 7.39,and fermentation time 36 h.Under the optimal conditions,the total antioxidant activity of fermentative product reaches 795.75 U.g-1?bean residue.The experimental result is basically agreed with the predicted value.
参考文献/References:
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LIU Ming,LUO Yuan-xu,NI Hui,et al.ACTIVE ANALYSIS OF SOYBEAN ANTIOXIDATIVE PEOTIDE FROM SOLID STATE FERMENTATION[J].Soybean Science,2007,26(03):381.[doi:10.3969/j.issn.1000-9841.2007.03.020]
备注/Memo
基金项目: 科技部“十一五”国家科技支撑重点资助项目(2006BAD02B01);集美大学创新团队基金资助项目。