[1]戴浩,周青.UV-B胁迫对大豆幼苗光呼吸的影响[J].大豆科学,2008,27(03):447-450.[doi:10.11861/j.issn.1000-9841.2008.03.0447]
　DAI Hao,ZHOU Qing.Effect of UV-B Stress on Photorespiration in Soybean Seedlings[J].Soybean Science,2008,27(03):447-450.[doi:10.11861/j.issn.1000-9841.2008.03.0447]
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UV-B胁迫对大豆幼苗光呼吸的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 447-450 栏目: 出版日期: 2008-06-25
Title:
Effect of UV-B Stress on Photorespiration in Soybean Seedlings
文章编号:
1000-9841(2008)03-0447-04
作者:
戴浩1; 周青1; 2
1江南大学环境与土木工程学院环境科学研究室，江苏 无锡 214122；
2江南大学工业生物技术教育部重点实验室，江苏 无锡 214122
Author(s):
DAI Hao1; ZHOU Qing1; 2
1 Laboratory of Environmental Science，School of Environmental and Civil Engineering，Jiangnan University，Wuxi 214122，Jiangsu;
2Key Laboratory of Industrial Biotechnology，Ministry of Education，Jiangnan University，Wuxi 214122，Jiangsu，China
关键词:
UV-B辐射; 大豆幼苗; 光呼吸速率
Keywords:
UV-B radiation; Soybean seedlings; Photorespiration rate
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0447
文献标志码:
A
摘要:
紫外辐射对农作物的生态效应是影响农业生产的重要问题。为研究紫外辐射对农作物光呼吸的影响，在温室模拟增强紫外辐射UV-B(280~320 nm)环境下，以大豆(Glycine max)为材料，用溶液培养试验法研究UV-B胁迫对大豆幼苗光呼吸速率(Pr)，光合速率(Pn)，乙醇酸氧化酶(GO)和谷胺酰胺合成酶(GS)的影响。结果表明：在UV-B胁迫下，光呼吸关键酶GO和GS活性显著提高，大豆幼苗的Pr显著提高，Pn显著降低，光呼吸关键酶GO和GS活性的显著提高导致了Pr的显著提高。T1和T2组Pr与Pn的动态变化表明，UV-B辐射与Pr、Pn间存在明显的剂量效应。因此，UV-B胁迫可以通过对光呼吸关键酶的影响调控光呼吸速率的变化。
Abstract:
As an important problem affecting agricultural productivity，ecological effect of UV-B radiation on crop seeds germination is highly concerned by researchers at domestic and abroad.To study the effect of UV-B radiation stress on photorespiration(Pr)，photosynthesis(Pn)，and the key enzyme of glycolate oxidase(GO)and glutamine synthetase(GS)in soybean seedlings.Soybean seedlings were exposed to two levels of ultraviolet-B radiation(UV-B，280~320 nm) under laboratory hydroponics conditions.Three ultraviolet radiant intensity treatment of 0(CK)，0.15 W.m-2(T1) and 0.45 W.m-2(T2)were exposed when the third euphylla of the soybean seedlings was expanded.Each treatment was irradiated six hours every day(9：00~15：00) and lasted for five days，then recoveried for ten days.Results showed that when seedlings were intimidated by UV-B，the key enzymes of photorespiration as glycolate oxidase and glutamine synthetase were effectively increased，so as photorespiration rate，while photosynthetic rate was effectively decreased，speculating the increasing of glycolate oxidase and glutamine synthetase led to the increasing of photorespiration rate.Obvious dosage effect was lied among UV-B radiation and glycolate oxidase and glutamine synthetase.Results suggest that the photorespiration rate could be regulated by key enzyme of photorespiration when under UV-B radiation stress.

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备注/Memo

基金项目：国家发改委稀土专项基金资助项目(IFZ20051210)。

作者简介：戴浩(1984-)，男，硕士研究生，研究方向为环境生态学。E-mail: daihao_1314@163.com。

更新日期/Last Update: 2014-10-08