[1]张小明,刘丽君,唐晓飞,等.中俄大豆种质遗传多样性分析[J].大豆科学,2008,27(01):15-20.[doi:10.11861/j.issn.1000-9841.2008.01.0015]
　ZHANG Xiao-ming,LIU Li-jun,TANG Xiao-fei,et al.Genetic Diversity of Soybean Germplasm in Russia and China[J].Soybean Science,2008,27(01):15-20.[doi:10.11861/j.issn.1000-9841.2008.01.0015]
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中俄大豆种质遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 15-20 栏目: 出版日期: 2008-02-25
Title:
Genetic Diversity of Soybean Germplasm in Russia and China
作者:
张小明1; 2; 刘丽君1; 唐晓飞1; 2; 杨喆1; 高明杰1; 张雷1; 蒲国峰1; 范海金3
1黑龙江省农业科学院大豆研究所，黑龙江哈尔滨 150086；
2东北农业大学，黑龙江哈尔滨 150086；3宁安农场，黑龙江宁安 157400
Author(s):
ZHANG Xiao-ming1; 2; LIU Li-jun1; TANG Xiao-fei1; 2; YANG Zhe1; GAO Ming-jie1; ZHANG Lei1; PU Guo-feng1; and FAN Hai-jin3
1Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；
2Northeast Agriculture University，Harbin 150086，Heilongjiang；
3Ning’an Farm,Ning’an 157400,Heilongjiang,China
关键词:
SSR; 野生大豆; 栽培大豆; 遗传多样性
Keywords:
SSR; Wild soybean; Cultivated soybean; Genetic diversity
DOI:
10.11861/j.issn.1000-9841.2008.01.0015
文献标志码:
A
摘要:
种质资源的扩增、改良和创新是解决大豆遗传基础狭窄的主要途径。利用SSR分子标记技术对来自俄罗斯和黑龙江省的82份野生大豆和东北四省区的39份栽培大豆材料进行遗传多样性分析，为种质资源利用和创新提供分子依据。在所合成的45对SSR引物中，12对引物扩增结果表现出良好的多态性，多态性位点共检测到50个等位基因，每个位点2~7个，平均4.17个，平均多态性信息量为0.595。聚类分析结果表明，在遗传相似系数0.734处，野生大豆和栽培大豆被明显的分开，与以往大豆属Soja亚属的形态学分类结果相一致，为野生大豆和栽培大豆分为两个种提供了分子水平上的依据。野生大豆和栽培大豆的平均遗传距离分别为0.2595和0.1895，表明野生大豆的遗传多样性比栽培大豆丰富。因此，可以利用俄罗斯和东北地区的野生大豆特有等位变异来扩大东北栽培大豆遗传多样性，进而拓宽东北大豆遗传基础。
Abstract:
The collection，improvement and innovation of germplasm resources are main ways to enrich the genetic basis of soybean.The genetic diversity of 84 accession of wild soybean from Russia and Heilongjiang province，China，and 39 accession of cultivated soybean from Northeast China，was studied by SSR technique.Forty-five SSR primers were synthesized according to published data，and 12 out of them had polymorphic alleles.A total of 50 alleles were observed，the number of alleles in different loci ranged from 2 to 7，averaging 4.17 per locus，the average polymorphism information content (PIC) was 0.595.Cluster analysis showed that wild and cultivated soybean belonged to two species from the dendgrom at the site of genetic similarity coefficient 0.734，which conformed to phenotypic results.The average genetic distance of wild soybean and cultivated soybean were 0.2595 and 0.1895 respectively，which suggested that wild soybean had more polymorphism than that of cultivated soybean.Hence，wild soybean from Russia and Northeast China could be used to broaden the genetic basis of cultivated soybean in Northeast China.

参考文献/References:

[1]Gai J Y， and Cui Z L.1994.Ancestral analysis of soybean cultivars released in china.Journal of Nanjing Agricultural University，17(3):19-23(盖钧镒，崔章林.1994.中国大豆育成品种亲本分析.南京农业大学学报，17(3):19-23)

[2]Guan R X. 2004.QTL mapping of soybean agronomic characters and genetic diversity analysis of soybean cultivars from China and Japan.Chinese Academy of Agriculture Sciences Post Doctoral Working Report，pp.43-63(关荣霞.2004.大豆重要农艺性状的QTL定位及中国大豆与日本大豆的遗传多样性分析.中国农业科学院博士后研究工作报告，pp.43-63)
[3]Hai L，Wang K J，and Yang K.2002.Genetic diversity of semi-wild soybean using SSR marker.Acta Botanica Boreali-Occidentalia Sinica，22(4):751-757(海林，王克晶，杨凯.2002.半野生大豆种质资源SSR位点遗传多样性分析.西北植物学报，22(4):751-757)
[4]Rongwen J，Akkayam M S，Bhagwat A A，Lavi U，and Cregan P B.1995.The use microsatellite DNA markers for soybean genotype identification.Theoretical and Applied Genetics，90(1):43-48
[5]Rogers S O，and Bendich A L.1994.Extraction of total cellular DNA from plants，algae and fungi.In：Gelvin S B，and Schilperoort A R.(eds).Plant Molecular Biology Manual.2nd ed.Dordrecht，The Netherlands，Rluwer press
[6]Wang B， Chang R Z，Tao L，Guan R X，Yan L，Zhang M H，Feng Z F，and Qiu L J.2003.Identification of SSR primer number for analyzing genetic diversity of Chinese cultivated soybean.Molecular Plant Breeding，1(1):82-88(王彪，常汝镇，陶莉，关荣霞，闫丽，张明恢，冯忠孚，邱丽娟.2003.分析中国栽培大豆遗传多样性所需SSR引物的数目.分子植物育种，1(1)：82-88)
[7]Wu X L， He C Y，Chen S Y，Zhuang B C，Wang K J，and Wang X C.2001.Phylogenetic Analysis of interspecies in Genus Glycine through SSR marker.Acta Genetics Sinica，28(4):359-366(吴晓雷，贺超英，陈受宜，庄炳昌，王克晶，王学臣.2001.用SSR分子标记研究大豆属种间亲缘进化关系.遗传学报，28(4):359-366)
[8]Zhao H K， Wang Y M，Li Q Y，Zhang M，and Zhuang B C.2001.SSR analysis of wild soybean(G.soja)and cultivated soybean from different latitude in China.Soybean Science，20(3):171-176(赵洪锟，王玉民，李启云，张明，庄炳昌.2001.中国不同纬度野生大豆和栽培大豆SSR分析.大豆科学，20(3):171-176)
[9]Zhou R， Zhang X J，Wang X Z，Sha A H，and Zhou X A.2006.Genetic diversity of soybean germplasm from Hubei province in China by SSR.Soybean Science，25(3):212-217(周蓉，张小娟，王贤智，沙爱华，周新安.2006.湖北省大豆种质资源的遗传多样性分析.大豆科学，25(3):212-217)
[10]Zhuang B C.2000.Genetic diversity of wild soybean in China and QTL mapping of quality traits.Dissertation for Ph D，China Agricultural University，Supervisor:Wang X K，pp.38-41(庄炳昌.2000.中国野生大豆的遗传多样性及品质性状的QTL定位，博士学位论文，中国农业大学，导师：王象坤，pp.38-41)

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备注/Memo

基金项目：引进国际先进农业科学技术计划948项目（2006-G5）

作者简介：张小明(1982-)，女，硕士研究生，研究方向为大豆遗传育种。
通讯作者(Corresponding author)：刘丽君， 研究员。E-mail:nkyssbd@126.com

更新日期/Last Update: 2014-10-08