[1]杨明俊,杨晓彤,杨庆尧.纳豆激酶的发酵和纯化方法[J].大豆科学,2007,26(06):961-965.[doi:10.3969/j.issn.1000-9841.2007.06.031]
　YANG Ming-jun,YANG Xiao-tong,YANG Qing-yao.FERMENTATION AND PURIFICATION TECHNIQUES IN NATTOKINASE PRODUCTION[J].Soybean Science,2007,26(06):961-965.[doi:10.3969/j.issn.1000-9841.2007.06.031]
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纳豆激酶的发酵和纯化方法

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 961-965 栏目: 出版日期: 2007-12-25
Title:
FERMENTATION AND PURIFICATION TECHNIQUES IN NATTOKINASE PRODUCTION
文章编号:
1000-9841(2007)06-0961-05
作者:
杨明俊1; 杨晓彤1; 杨庆尧2
1. 上海师范大学微生物与免疫学研究所，上海 200234;
2.上海杨杨百草研究所，上海 200234
Author(s):
YANG Ming-jun1; YANG Xiao-tong1; YANG Qing-yao2
1. Institute of Microbiology & Immunology，Shanghai Normal University，Shanghai 200234；
2.Shanghai Yang’s Herb Institute，Shanghai 200234
关键词:
纳豆激酶; 发酵; 育种; 分离纯化
Keywords:
Nattokinase; Fermentation; Breeding; Extraction
分类号:
q814
DOI:
10.3969/j.issn.1000-9841.2007.06.031
文献标志码:
A
摘要:
对纳豆激酶在菌种选育、发酵条件及分离纯化工艺方面的研究进展作了综述，介绍了提高纳豆激酶产量的多种固、液体发酵条件和菌种选育结果，并归纳了膨胀床吸附、金属螯合双水相亲和纳豆分配、反胶束萃取、磁性微球分离等分离技术在纳豆激酶分离纯化中的应用。
Abstract:
This article introduced the progresses of strain breeding，solid and liquid fermentation and purification in nattokinase production.Various studies on fermentation conditions and breeding efforts to boost nattokinase production were reviewed，and the application of separation techniques for nattokinase extraction，such as expanded bed adsorption，immobilized metal affinity partition，reverse micelles extraction and magnetic bead separation were also summarized.

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备注/Memo

基金项目：上海杨杨百草研究所资助

作者简介：杨明俊(1979-)，男，硕士研究生，研究方向为微生物药物学。
通讯作者：杨晓彤。Tel：021-64322895， E-mail：xtyang@shnu.edu.cn

更新日期/Last Update: 2014-10-20