[1]马跃华,董 超,杨 明,等.纳豆激酶的亲和吸附工艺研究[J].大豆科学,2010,29(02):306-309.314.[doi:10.11861/j.issn.1000-9841.2010.02.0306]
　MA Yue-hua,DONG Chao,YANG Ming,et al.Adsorption of Nattokinase by Soybean Microspheres[J].Soybean Science,2010,29(02):306-309.314.[doi:10.11861/j.issn.1000-9841.2010.02.0306]
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纳豆激酶的亲和吸附工艺研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年02期 页码: 306-309.314 栏目: 出版日期: 2010-04-25
Title:
Adsorption of Nattokinase by Soybean Microspheres
文章编号:
1000-9841（2010）02-0306-04
作者:
马跃华1; 2; 董 超2; 杨 明1; 2; 史延茂2
1.河北工业大学 化工学院生物工程系, 天津 300130 ；
2.河北省生物研究所, 河北 石家庄 050081
Author(s):
MA Yue-hua1; 2;  DONG Chao2; YANG Ming1; 2;  SHI Yan-mao2
1.College of Chemistry and Chemical Engineering, Hebei University of Technology, Tianjin 300100;?
2. Hebei Institute of Biology, Shijiazhuang 050081,Hebei，China
关键词:
大豆颗粒; 纳豆激酶; 吸附平衡; 吸附动力学
Keywords:
Soybean microspheres;  Nattokinase;  Adsorption equilibrium;  Adsorption dynamics
分类号:
Q814
DOI:
10.11861/j.issn.1000-9841.2010.02.0306
文献标志码:
A
摘要:
采用静态吸附方法验证了大豆颗粒对纳豆激酶的亲和吸附特性，测定了其静态吸附动力学特性和吸附等温线以及一些吸附条件。结果表明：该亲和吸附等温线符合Langmiur方程，吸附动力学符合扩散方程；吸附的最佳缓冲液选择pH6.0、0.01 mol·L-1的PBS，在静态时选用大豆颗粒的最大吸附量为6351.58 IU·g-1，洗脱后收率达到81.30%，纯化倍数约30.23倍。初步推断大豆蛋白中含有与纳豆激酶特异性吸附的的配体结构。
Abstract:
The affinity adsorption of Nattokinase by soybean microspheres was characterized and the static adsorption dynamics, adsorption isotherms and some adsorption conditions were investigated in the present work. The results revealed that the adsorption isotherm was accorded with Langmuir adsorption isotherm and the adsorption dynamics was coincident with diffusion equation. The best buffer was 0.01 mol·L-1PBS with pH of 6.0.Under this condition,the maximum adsorption capacity was 6 351.58 IU·g-1. The purification fold and recovery of Nattokinase activity reached 30.23 and 81.3%,respectively. It may be presumed that the soybean protein contains the ligand which absorb the nattokinase specifically.

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备注/Memo

基金项目：河北省科技支撑计划资助项目 （08276413D）。

作者简介：马跃华（1982-），女，硕士，研究方向为微生物制药。
通讯作者：史延茂，研究员。E-mail：yanmaosh@163.com。

更新日期/Last Update: 2014-09-13