[1]陈丽霞,李英慧,郑服丛,等.油菜素内酯（BR）对大豆疫霉根腐病抗性的影响[J].大豆科学,2007,26(05):713-717,727.[doi:10.3969/j.issn.1000-9841.2007.05.015]
　CHEN Li-xia,LI Ying-hui,ZHENG Fu-cong,et al.EFFECT OF BRASSINOSTEROIDS ON SOYBEAN RESISTANCE TO PHYTOPHTHORA SOJAE[J].Soybean Science,2007,26(05):713-717,727.[doi:10.3969/j.issn.1000-9841.2007.05.015]
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油菜素内酯（BR）对大豆疫霉根腐病抗性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 713-717,727 栏目: 出版日期: 2007-10-25
Title:
EFFECT OF BRASSINOSTEROIDS ON SOYBEAN RESISTANCE TO PHYTOPHTHORA SOJAE
文章编号:
1000-9841(2007)05-0713-05
作者:
陈丽霞1; 2; 李英慧2; 郑服丛1; 任朝阳3; 关荣霞2; 刘章雄2; 郝再彬3; 常汝镇2; 邱丽娟2
1.华南热带农业大学, 儋州 571737；
2.中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京100081；
3.东北农业大学生命科学学院，哈尔滨 150030
Author(s):
CHEN Li-xia1; 2;  LI Ying-hui2;  ZHENG Fu-cong1;  REN Zhao-yang3; GUANRong-xia2; LIU Zhang-xiong2; HAO Zai-bin3; CHANG Ru-zhen2; QIU Li-juan2
1. South China University of Tropical Agriculture, Danzhou 571737;
2.National Key Facility of Gene Resources and Genetic Improvement / Key Lab of Crop Germplasm Resources & Biotechnology, the Ministry of Agriculture, Institute of Crop Science, CAAS, Beijing 100081;
3.Life Science College,Northeast Agriculture University, Harbin 150030.
关键词:
油菜素内酯; 酶活性; 大豆; 疫霉根腐病
Keywords:
Brassinosteroids (BR);  Enzyme activity;  Soybean;  Phytophthora root rot
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.05.015
文献标志码:
A
摘要:
以抗、感疫霉根腐病的2个品种和一对矮秆突变体与野生型为材料，研究BR对抗大豆疫霉根腐病防御反应的过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）活性的影响。目的是明确BR对抗大豆疫霉根腐病的作用效果，鉴定矮秆突变体的抗性。酶活测定表明，接种使大豆叶片的 POD和PAL活性显著增强，说明病原物诱导使植株产生系统抗病性；加BR接种处理（B+P+）比加BR不接种处理（B+P-）的POD和PAL活性有一定的增强，表明BR对抗大豆疫霉根腐病有一定的抗性效果；挑战接种鉴定表明，东农42属于感病，而东泽11属于中间类型，施加BR后对这两个品种的抗性均有增强作用。
Abstract:
Peroxidase (POD) and phenylalanine ammonialyase (PAL)are defensive enzyme for soybean Phytophthora root rot. To elucidate the effect of Brassinosteroids (BR)on soybean resistance to Phytophthora root rot and identify the resistance of a soybean mutant. Two soybean cultivars Williams(sensitive) and Ludou 4(resistant), and a pair of soybean mutant(Dongze 11) with its wild type(Dongnong 42) were adopted as materials. The soybean seedlings were treated with Brassinosteroids (BR)or/and inoculated with Phytophthora sojae and the changes in activities of POD and PAL were investigated. The POD and PAL activity in plants inoculated with Phytophthora sojae were significantly higher than those without inoculation, which showed the Phytophthora sojae induced the systemic resistance of soybean plants. The activity of POD and PAL were also increased when treated with BR, which suggested that BR might have some resistant effect on Phytophthora root rot. The identification results showed Dongnong 42 was susceptible and the mutant was mid-type, their resistance to Phytophthora root rot were enhanced when treated with BR.

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备注/Memo

基金项目：国家863项目(2006AA100104)；国家自然科学基金重大项目(30490250)

作者简介：陈丽霞（1981-），女，硕士，主要从事大豆病害研究。E-mail:chenlixia0614@163.com
通讯作者：邱丽娟博士，研究员，博士生导师。Tel: 010-62135623; E-mail: qiu_lijuan@263.net

更新日期/Last Update: 2014-10-20