[1]刘利新,华菁,任申玥,等.降解大豆分离蛋白AMPS接枝共聚物的合成与表征[J].大豆科学,2007,26(04):591-596.[doi:10.3969/j.issn.1000-9841.2007.04.027]
　LIU Li-xin,HUA Jing,REN Shen-yue,et al.SYNTHESIS AND CHARACTERIZATION OF GRAFT COPOLYMERS OF HYDROLYTIC SOY PROTEIN ISOLATED AND AMPS[J].Soybean Science,2007,26(04):591-596.[doi:10.3969/j.issn.1000-9841.2007.04.027]
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降解大豆分离蛋白AMPS接枝共聚物的合成与表征

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年04期 页码: 591-596 栏目: 出版日期: 2007-08-25
Title:
SYNTHESIS AND CHARACTERIZATION OF GRAFT COPOLYMERS OF HYDROLYTIC SOY PROTEIN ISOLATED AND AMPS
文章编号:
1000-9841(2007)04-0591-06
作者:
刘利新; 华菁; 任申玥; 刘燕; 陈明清; 杨成; 刘晓亚
江南大学化学与材料工程学院，无锡 214122
Author(s):
LIU Li-xin; HUA Jing; REN Shen-yue; LIU Yan; CHEN Ming-qing; YANG Cheng; LIU Xiao-ya
Department of Chemical and Material Engineering of Southern Yangtze University，Wuxi 214122
关键词:
大豆分离蛋白; 降解; 接枝共聚; 2-丙烯酰胺基-2-甲基丙磺酸; 溶液性能
Keywords:
Soy protein Isolated; Hydrolysis; Graft copolymerization; 2-Acrylanmido-2-methyl propane sulfonic acid
分类号:
Q816 S565.1
DOI:
10.3969/j.issn.1000-9841.2007.04.027
文献标志码:
A
摘要:
利用酸性蛋白酶对大豆分离蛋白（SPI）进行降解，得到一系列降解大豆分离蛋白（HSPI）。以过硫酸铵为引发剂、2-巯基乙醇为蛋白质变性剂，在浓度为8 mol/L尿素溶液中进行HSPI与水性单体2-丙烯酰胺基-2-甲基丙磺酸（AMPS）的接枝共聚，得到接枝产物（HSPI-g-PAMPS）。研究了水解度、引发剂用量、反应时间和单体用量对接枝率和接枝效率的影响，并对HSPI和HSPI-g-PAMPS的溶液性能进行了研究。结果表明：HSPI-g-PAMPS的Zeta电位明显降低、在低剪切速率下HSPI-g-PAMPS的粘度最大。
Abstract:
A series of the hydrolytic Soy Proteins Isolated (HIPS)were obtained by the enzymatic hydrolysis of Soybean Protein Isolated (SPI)using the acid proteolytic enzyme.The graft copolymers(HSPI-g-PAMPS)of the hydrolytic Soybean Protein Isolated (HSPI)and AMPS had been prepared in 8 mol/ L urea aqueous solution under nitrogen atmosphere ，using ammonium persulphate(APS)as an initiator，β-mercaptoethanol(ME)as a protein unfolding agent .Evidence of HSPI-g-PAMPS was obtained by comparison of IR Spectro of HSPI and HSPI-g-PAMPS.The effect of time，initiator concentration，degree of hydrolysis and monomer concentration on the graft copolymerization have been studied.The properties of HSPI-g-PAMPS have been characterized and it was shown that the Zeta-potential of HSPI-g-PAMPS was lowest than HSPI-Besides，the viscosity of HSPI-g-PAMPS was the highest under the low cut rate.

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备注/Memo

基金项目：国家自然科学基金资助项目(NSFC20374025)

作者简介：刘利新（1982－），男，硕士研究生，从事大豆分离蛋白接枝改性研究。Tel:013961798581;E-mail:liulixin821027@hotmail.com
通讯作者：刘晓亚，教授，博士生导师，Tel: 0510-89880780，E-mail:lxy@sytu.edu.cn

更新日期/Last Update: 2014-10-21