[1]王利民,秦贵信,刘林娜,等.三种亲和层析体系纯化大豆凝集素的比较研究[J].大豆科学,2007,26(01):51-54.[doi:10.3969/j.issn.1000-9841.2007.01.012]
　WANG Li-min,QIN Gui-xin,LIU Lin-na,et al.COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION[J].Soybean Science,2007,26(01):51-54.[doi:10.3969/j.issn.1000-9841.2007.01.012]
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三种亲和层析体系纯化大豆凝集素的比较研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年01期 页码: 51-54 栏目: 出版日期: 2007-02-25
Title:
COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION
文章编号:
1000-9841(2007)01-0051-04
作者:
王利民; 秦贵信; 刘林娜; 张亮亮; 张玲玲
吉林农业大学动物科技学院， 长春 130118
Author(s):
WANG Li-min; QIN Gui-xin; LIU Lin-na; ZHANG Liang-liang; ZHANG Ling-ling
College of Animal Science and Technology，Jilin Agricultural University，Changchu 130118
关键词:
亲和层析; 纯化; 大豆凝集素
Keywords:
Affinity chromatography; Purification; Soybean agglutinin
分类号:
S565.101
DOI:
10.3969/j.issn.1000-9841.2007.01.012
文献标志码:
A
摘要:
用N-乙酰基-D-半乳糖胺-epoxy-Sepharose 6B、半乳糖胺-CH-Sepharose 4B和瓜尔胶三种亲和层析体系对大豆中的凝集素进行分离提纯，并进行了多方面的比较。结果显示：三种亲和层析体系纯化大豆凝集素的产率存在显著差异，依次分别为147±15、110±12和128±16mg/100g生大豆；三种亲和胶的亲和饱和度差异显著，分别为12.2±1.4、7.5±1.3和2.1±1.6 mg/mL凝胶。这一结果表明大豆凝集素最理想的纯化方法是N-乙酰基-D-半乳糖胺-expoxy-sepharose 6B亲和层析体系。
Abstract:
Purification of soybean agglutinin with three affinity chromatography systems， GalNac- epoxy-Sepharose 6B，Gal-CH-Sepharose 4B and guar were compared.The results showed the differences of the prodution rate and absorbing capcities among the three systems were significant， 147±15，110±12， 128±16mg/100g raw soybean and 12.2±1.4，7.5±1.3， 2.1±1.6 mg/mL gel separately.This result indicated that the best way to purify soybean agglutinin was GalNac- epoxy-Sepharose 6B.

参考文献/References:

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备注/Memo

基金项目：国家自然基金（C020302）资助项目

作者简介：王利民（1966-），男，在读博士，副教授，研究方向为动物营养。Tel:13944188861

更新日期/Last Update: 2014-10-21