Zhou Rong Zhang Xiaojuan Wang Xianzhi Sha Aihua Zhou Xinan T u Ganying.GENETIC DIVERSITY OF SOYBEAN GERMPL ASM FROM HUBEI PROVINCE IN CHINA BY SSE[J].Soybean Science,2006,25(03):212-217.[doi:10.11861/j.issn.1000-9841.2006.03.0212]
湖北省大豆种质资源的遗传多样性分析*
- Title:
- GENETIC DIVERSITY OF SOYBEAN GERMPL ASM FROM HUBEI PROVINCE IN CHINA BY SSE
- Keywords:
- Soybean; Germplasm ; SSR; Genetic diversity
- 文献标志码:
- A
- 摘要:
- 利用 SSR标记和 UPGMA 聚类分析, 对 64 份湖北省大豆种质资源进行遗传多样性分析。结果表明 ,27 对 SSR引物扩增出 166 条多态性带 ,SSR 的遗传多样性指数分布范围 ,Simpson 指数为 0. 2406~ 0. 9037,平均值 0. 6960 ;Shannon-weaver 指数为 0. 4800 ~ 2. 4684,平均值 1. 4538 。聚类分析将湖北省地方大豆品种分为 3 个类群 ,包括鄂中南江汉平原大豆类群,鄂西北大豆类群以及鄂东 、鄂南和鄂西混合类群。江汉平原大豆地方品种表现出较高的遗传多样性水平 。
- Abstract:
- T here were more than one thousand local soybean [ Glycine max (L.) Merr. ] germplasms inHubei province. However , their diversity was not well characterized. The objective of this study was toquantify genetic diversity of sixty-four soybean landraces from Hubei by SSR and cluster analysis with theUPGMA method. 166 polymorphic bands were amplified by twenty-seven pairs SSR primers. Genetic di-versity index showed that distribution of Simpson index was 0. 2406~ 0. 9037 and mean 0. 6960, and distri-bution of Shannon-weaver index was 0. 4800 ~ 2. 4684 and mean 1. 4538. By using the dendrogram analy-sis, 64 landraces were separated into three major groups at the DNA level :Jianghan alluvial plain group,northwest Hubei group and mixed group of the east , south and west of Hubei province. The diversity levelof soybean landrace from Jianghan alluvial plain was higher than that of other landraces.
参考文献/References:
1 王珍, 方宣钧. 植物 DNA 分离[ J] . 分子植物育种, 2003, 1(2):281 - 288.
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备注/Memo
基金项目:国家“863”重大专项(2002AA207007);农业部油料作物遗传改良重点开放实验室资助项目。