[1]王 　 萍　 王 　 罡　 季 　 静.不同菜用大豆品种的胚胎发生与再生*[J].大豆科学,2005,24(04):314-316.[doi:10.11861/j.issn.1000-9841.2005.04.0314]
　Wang Ping Wang Gang Ji Jing.EMBRYOGENESIS AND REGENERATION FROM DIFFERENT OF VEGETABLE SOYBEAN[J].Soybean Science,2005,24(04):314-316.[doi:10.11861/j.issn.1000-9841.2005.04.0314]
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不同菜用大豆品种的胚胎发生与再生*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年04期 页码: 314-316 栏目: 出版日期: 2005-11-25
Title:
EMBRYOGENESIS AND REGENERATION FROM DIFFERENT OF VEGETABLE SOYBEAN
作者:
王 　 萍1　 王 　 罡2　 季 　 静2
(1. 淮海工学院海洋学院 ,连云港 222005;2. 天津大学农业与生物工程学院 ,天津 300072)
Author(s):
Wang Ping 1 　 Wang Gang 2 　 Ji Jing 2
(1. Huaihai Institute of Technology , Marine School , Lianyungang 222005;2. TianjinUniversity , Agriculture &Bioengineering College , Tianj in 300072)
关键词:
菜用大豆; 未成熟子叶; 胚胎发生; 再生植株
Keywords:
Vegetable soybean ; Immature cotyledon; Embryogenesis; Regenerated plantlet
DOI:
10.11861/j.issn.1000-9841.2005.04.0314
文献标志码:
A
摘要:
以 8 个菜用大豆品种的未成熟子叶为外植体,在 MS(附加高浓度 2, 4 - D)培养基诱导愈伤组织形成、体细胞胚胎发生与再生植株。结果表明 :八个菜用大豆品种的未成熟子叶均可诱导产生愈伤组织与体细胞胚胎发生, 出愈率为 14. 71%～ 100%。体细胞胚胎发生率变化在 0～ 36. 76%之间,绿子叶菜用大豆没有形成体细胞胚。体细胞胚可正常萌发产生小植株 ,移栽成活结实 。菜用大豆的出愈率和体细胞胚胎发生率与基因型有关。
Abstract:
Callus formation, somatic embryogenesis and plant regeneration were induced by MS medium in-cluding higher concentrations of 2 ,4- D with immature cotyledon of eight genotypes in vegetable soybean.The results showed that callus formation and somatic embryogenesis of eight genotypes of vegetable soy-bean with immature cotyledon were induced successfully. Frequencies of callus formation varied from14. 7 %to 100%and frequencies of somatic embryogenesis changed 0 ～ 36. 76%. Somatic embryo has notbeen induced from variety Luziyecaidou with green cotyledon. Somatic embryos could germinate , regener-ate plantlets and seed. The frequencies of callus and somatic embryogenesis were associated with genotypesin vegetable soybean.

参考文献/References:

1　 韩天富, 盖钧镒. 世界菜用大豆生产、贸易和研究的进展[ J] . 大豆科学, 2002, 21(4):278 - 284.

2　 韩天富. 中国菜用大豆的种植制度的品种类型[ J] . 大豆科学,2002, 21(2):83- 87.
3　 王萍, 王罡, 季静, 等. 大豆体细胞胚胎发生与农杆菌介导的遗传转化[ J] . 遗传, 2004, 26(5):695- 700.
4　 Wang Ping , Wang Gang , Ji Jing , et al. A novel sy stem for Pro-liferation, maintenance and plantlet Germination from somaticembryo of soybean[ J] . Acta Botanica Sinica , 2004, 46 (2):154-158.
5　 Parrott WA , William EG , Hildebrand DF , et al. Effect of geno-ty pe on somatic embry ogenesis from immature cotyledons of soy-bean[ J] . Plant Cell Tissue and Organ Culture, 1989, 16:15 -21.
6　 周思君, 尹光初. 从大豆幼胚诱导胚胎发生再生植株[ J] . 大豆科学, 1989, 8(1):39- 45.

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备注/Memo

基金项目:国家植物转基因中试及产业化基地专项基金(项目编号 J99 - B - 001)　 作者简介:王萍(1957 -), 女, 教授, 博士, 主要从事生物技术与植物转基因的研究。E - mail:y - pwang @yahoo. com. cn

更新日期/Last Update: 2017-01-13