Liu Yag uangWang JicaiYang Qingkai.PRELIMINARY STUDIES ON THE TOXIN FROMCERCOSPOR A SOJ IN A HARA[J].Soybean Science,1999,18(04):306-311.[doi:10.11861/j.issn.1000-9841.1999.04.0306]
大豆灰斑病菌( Cercospora sojina Hara)毒素的初步研究
- Title:
- PRELIMINARY STUDIES ON THE TOXIN FROMCERCOSPOR A SOJ IN A HARA
- 文献标志码:
- A
- 摘要:
- 本试验主要从液体培养基的种类、培养温度、培养方式、培养天数、接种量和培养基的pH值及碳源等几个方面入手研究了大豆灰斑病菌最佳的产毒条件。利用大豆胚根伸长抑制率和单复叶萎蔫指数来测定毒素的产量。实验结果得出该菌的最佳产毒条件是利用Czapek培养基,以蔗糖作为碳源,糖含量为3% ,将培养基的pH调到6- 7,接种三块直径为6mm的新鲜菌丝块,于25- 28℃下静止培养25- 27天。
- Abstract:
- The condi tions of producing toxin f rom Cercospora sojina Hara w ere systema ticallystudies on following apsects: kind of liquid culture medium, culture temperture, culture time,incubation mode, inoculation quantity and pH v alue of culture medium. The inhibi tion ofsoybean radicle grow th and w ilting index of single compound leaf of soybean w ere used totest the injury results of foxicity. The experimental result s show ed that the optimun co nditionsof producing toxin f rom Cercospora sojina Hara w ere Czapek ’s liquid medium, the pHvalue 6 to 7, three pieces (φ= 6mm) mycelia, 25- 28℃ , static incubatio n, 25- 27 days underdark and 3% sucrose as C soure respectivelly.
参考文献/References:
[1 ] 黄桂潮、霍虹, 1984,大豆灰斑病菌(Cerospor a so jina Hara)生理小种鉴定初报,大豆科学, 3( 3): 231- 234
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