[1]李东坡,韩岚岚,张旭霞,等.大豆食心虫滞育期间体内代谢酶对温度诱导的响应[J].大豆科学,2018,37(03):415-423.[doi:10.11861/j.issn.1000-9841.2018.03.0415]
　LI Dong-po,HAN Lan-lan,ZHANG Xu-xia,et al.Metabolic Response of the Soybean Pod Borer (Leguminirora glycinioorella) During Diapause to Temperature[J].Soybean Science,2018,37(03):415-423.[doi:10.11861/j.issn.1000-9841.2018.03.0415]
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大豆食心虫滞育期间体内代谢酶对温度诱导的响应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年03期 页码: 415-423 栏目: 出版日期: 2018-05-20
Title:
Metabolic Response of the Soybean Pod Borer (Leguminirora glycinioorella) During Diapause to Temperature
作者:
李东坡; 韩岚岚; 张旭霞; 赵奎军; 张文霖; 高云雷; 邓世群; 张傲楠
（东北农业大学 农学院，黑龙江 哈尔滨 150030）
Author(s):
LI Dong-po;  HAN Lan-lan;  ZHANG Xu-xia;  ZHAO Kui-jun;  ZHANG Wen-lin;  GAO Yun-lei;  DENG Shi-qun;  ZHANG Ao-nan
(College of Agriculture, Northeast Agricultural University, Harbin 150030, China)
关键词:
滞育; 大豆食心虫; 诱导温度; 代谢酶; 酶活
Keywords:
Diapause;  Soybean pod borer;  Induction temperatures;  Metabolic enzymes;  Enzyme activity
DOI:
10.11861/j.issn.1000-9841.2018.03.0415
文献标志码:
A
摘要:
为明确大豆食心虫在未滞育-滞育-滞育解除过程的代谢变化，准确测报大豆食心虫的发生时期，掌握防治时机，进而达到减少使用农药的目的，本试验研究了经4，13，18，23，25℃及室外自然温度不同温度处理后的大豆食心虫Leguminirora glycinioorella滞育前及进入滞育后，不同阶段其体内抗冻、滞育主要代谢酶－海藻糖酶、山梨醇脱氢酶、丙酮酸激酶、过氧化物酶活力的变化。结果表明：自然状态下进入滞育的大豆食心虫体内上述4种代谢酶的活力与未滞育时这4种代谢酶的活力均差异显著，海藻糖酶、山梨醇脱氢酶、丙酮酸激酶活力分别下降0.99，2.85和7.86 U·mg-1，过氧化物酶活力升高1.58 U·mg-1；与室外自然条件相比，4℃诱导大豆食心虫进入滞育初期，其体内海藻糖酶和丙酮酸激酶活力显著降低，过氧化物酶活力显著升高，而山梨醇脱氢酶活力在各诱导温度下保持平稳；不同滞育诱导温度处理大豆食心虫进入滞育后，在滞育初期（11月）、滞育中期（2月）、滞育解除（6月）代谢酶活力变化明显，其中23℃诱导的处理组在这3个滞育阶段的海藻糖酶、山梨醇脱氢酶、丙酮酸激酶活力均达到峰值，2月是大豆食心虫由滞育向滞育解除转折的关键期。研究结果可以为今后大豆食心虫的发生测报提供依据。
Abstract:
The purpose was to clarify the metabolic changes during the period of nondiapause-diapause-diapause elimination of soybean pod borer (Leguminirora glycinioorella), measure the occurrence period of soybean pod borer accurately, grasp the timing of prevention and control, and then achieve the purpose of reducing the use of pesticides. Trehalase, sorbitol dehydrogenase, pyruvate kinase and peroxidase are main metabolic enzymes related to antifreeze and diapause of soybean pod borer. This study investigated the changes of those enzyme activity in soybean pod borer before and after diapause, treated with different diapause-inducing temperatures and different stages of diapause period at different temperatures(4, 13, 18, 23, 25℃ and outdoor). The results showed that the activity of the four metabolic enzymes of soybean pod borer diapaused under natural conditions were all significantly different compared with the pre-diapause activity of soybean pod borer. Among them, the activity of trehalase, sorbitol dehydrogenase, pyruvate kinase decreased by 0.99, 2.85 and 7.86 U·mg-1, respectively, the peroxidase activity increased by 1.58 U·mg-1. Compared with outdoor natural conditions, early diapause after 4℃ induction treatment, the activity of trehalase and pyruvate kinase were significantly decreased while the activity of peroxidase was significantly increased, however, the activity of sorbitol dehydrogenase remained stable under all induction temperatures. In different temperatures conditions, the activity of metabolic enzymes of soybean pod borer in early diapause(November), mid-diapause(February) and after the release of diapause(June) were significantly different. Among them, in the treatment group induced by 23℃, the trehalase, sorbitol dehydrogenase andpyruvate kinase enzyme activities peaked in those three diapause stages, February is the critical period when soybean pod borer changes from diapause to diapause termination.These results would provide basis for the occurrence and forecast of soybean pod borer in the future.

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备注/Memo

收稿日期：2018-01-19

基金项目：现代农业产业技术体系建设专项（CARS-04）。
第一作者简介：李东坡（1991-），男，硕士，主要从事昆虫生理与生态研究。E-mail: dongpoli88@163.com。
通讯作者：赵奎军（1960-），男，博士，教授，主要从事昆虫综合防治研究。E-mail: kjzhao@163.com。
韩岚岚（1970-），女，博士，副教授，主要从事昆虫生物化学与分子生物学研究。E-mail:hanll_neau@aliyun.com。

更新日期/Last Update: 2018-06-08