[1]李娜,曹越平.转基因大豆SHZD32-01对草甘膦的抗性及草甘膦除草效果研究[J].大豆科学,2018,37(03):408-414.[doi:10.11861/j.issn.1000-9841.2018.03.0408]
　LI Na,CAO Yue-ping.Resistance of Transgenic Soybean SHZD32-01 to Glyphosate and Glyphosate Weeding Control in Fields[J].Soybean Science,2018,37(03):408-414.[doi:10.11861/j.issn.1000-9841.2018.03.0408]
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转基因大豆SHZD32-01对草甘膦的抗性及草甘膦除草效果研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年03期 页码: 408-414 栏目: 出版日期: 2018-05-20
Title:
Resistance of Transgenic Soybean SHZD32-01 to Glyphosate and Glyphosate Weeding Control in Fields
作者:
李娜; 曹越平
（上海交通大学 农业与生物学院，上海 200000）
Author(s):
LI Na;  CAO Yue-ping
(College of Agricultural and Biological, Shanghai Jiao Tong University，Shanghai 200000, China)
关键词:
转基因大豆SHZD32-01; 不同浓度; 草甘膦; 抗性; 除草效果
Keywords:
Transgenic soybean SHZD32-01;  Different concentration;  Glyphosate;  Resistance;  Weeding control effect
DOI:
10.11861/j.issn.1000-9841.2018.03.0408
文献标志码:
A
摘要:
为了评价自主研制的转G10-EPSPS基因的抗除草剂转基因大豆SHZD32-01对不同浓度草甘膦的耐受性和草甘膦的除草效果，以SHZD32-01大豆为试验材料，以受体中豆32为对照，采用随机区组设计，在V3期喷施田间剂量为1，2，4倍浓度的草甘膦（1.23，2.46，4.92 kg·hm-2）和清水，统计大豆株高和受害率等指标，以及草甘膦喷施后各小区再次长出杂草的密度与高度。结果表明：1~4倍田间浓度的草甘膦处理下，转基因大豆SHZD32-01的株高和覆盖度之间无显著差异，对转基因大豆的生长没有明显影响，而受体材料全部死亡。清水处理下，SHZD32-01与中豆32之间的株高无显著差异，但株高低于喷施草甘膦的处理。喷施草甘膦7 d后，SHZD32-01与受体中豆32的种植小区内杂草全部死亡；28 d后田间浓度草甘膦处理下再次长出杂草的密度和高度与人工除草小区无显著差异。田间种植转基因大豆SHZD32-01在V3期喷施1倍草甘膦即可达到很好的除草效果。
Abstract:
In order to assess the resistance of independently developed transgenic soybean SHZD32-01(G10-epsps ) to different concentrations of glyphosate and the weeding control effect, SHZD32-01 and the receptor Zhongdou 32 were treated by 1,2,4 fold of the recommended concentration of glyphosate(1.23,2.46,4.92 kg·ha-1） and water,with four replications in a 4×4 completely randomized block design. The height and damage rate of soybean plants were analyzed, and density and height of the regenerate weeds were surveyed. The result showed that there were no significant differences between the height of SHZD32-01 treated by different glyphosate concentrations, as well as the height of SHZD32-01 and Zhongdou 32 treated by water. The height of SHZD32-01 treated by water was lower than that of treated by glyphosate(1-4 fold of glyphosate). After 7 d, weeds treated by glyphosate treatment could not survive in the field of SHZD32-01 and Zhongdou 32. After 28 d, there were no significant differences between the density and height of regenerated weeds in the field of SHZD32-01 treated by mannual weeding and recommended glyphosate concentration. To achieve ideal effect, 1.23 kg·ha-1

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备注/Memo

收稿日期：2017-12-11

基金项目：国家转基因重大专项（2016ZX08004001-004）。
第一作者简介：李娜（1993-），女，硕士，主要从事抗草甘膦大豆的遗传转化与抗性鉴定研究。E-mail：ln254251788@sjtu.edu.cn。
通讯作者：曹越平（1967-），女，博士，教授，主要从事转基因大豆新品种培育研究。E-mail：yuepingcao@ sjtu.edu.cn。

更新日期/Last Update: 2018-06-08