[1]沈洁,田丹,蔡燕,等.EDTA对大豆脂氧酶的活性和催化体系的影响[J].大豆科学,2017,36(02):305-309.[doi:10.11861/j.issn.1000-9841.2017.02.0305]
　SHEN Jie,TIAN Dan,CAI Yan,et al.Effects of EDTA on the Soybean Lipoxygenase Catalytic System[J].Soybean Science,2017,36(02):305-309.[doi:10.11861/j.issn.1000-9841.2017.02.0305]
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EDTA对大豆脂氧酶的活性和催化体系的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年02期 页码: 305-309 栏目: 出版日期: 2017-03-20
Title:
Effects of EDTA on the Soybean Lipoxygenase Catalytic System
作者:
沈洁1; 田丹2; 蔡燕2; 陈娇2; 赵勤2; 吴锦明2
1.江南大学 化学与材料工程学院，江苏 无锡 214122；?
2.南通大学 化学化工学院，江苏 南通 226019
Author(s):
SHEN Jie1;  TIAN Dan2;  CAI Yan2;  CHEN Jiao2;  ZHAO Qin2;  WU Jinming2
1.School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China;
2.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China
关键词:
脂氧酶; EDTA; 稳定性; 催化
Keywords:
Lipoxygenase;  EDTA;  Stability;  Catalysis
DOI:
10.11861/j.issn.1000-9841.2017.02.0305
文献标志码:
A
摘要:
本文研究了EDTA对脂氧酶（LOX）溶液稳定性及催化行为的影响。结果表明：EDTA对脂氧酶的稳定性具有显著的促进作用，室温下能在较长时间（30 d）内保持较高的酶活（80%以上），改变了其易失活的特点。EDTA能大幅度提高脂氧酶催化亚油酸的催化效率，使其反应进程缩短为原来的1/3，但当底物为大豆油时起抑制作用。光谱试验显示：EDTA对LOX的影响只是局部构象的微调，并不涉及二级结构和三级结构的改变。EDTA在保存液体脂氧酶和催化氧化亚油酸的反应中有较大的应用价值。
Abstract:
The effects of EDTA on the stability and catalytic behavior of lipoxygenase(LOX) were studied in this paper The results showed that EDTA could improve the stability of LOX significantly, and it could retain high activity(80% or more) in a long time(30 days) at room temperature EDTA can greatly improve the catalytic efficiency of LOX catalyzed on linoleic acid, so that the reaction process can be reduced to 1/3 of the original But it would inhibit the catalytic activity when the substrate is soybean oil Spectral experiments show that EDTA induces only a fine tuning of the local conformation of LOX, and does not involve the change of the secondary structure or tertiary structure EDTA has great application value in preserving liquid LOX and catalytic oxidation of linoleic acid

参考文献/References:

［1］Stephen J B，Sharon H A S Perspective on enzyme catalysis［J］. Science, 2003, 301: 1196-1202

［2］Wheeler D H, Milun A, Linn F Dimer Acid Structures: Cyclic structures of clay catalyzed dimers of normal linoleic 9 cis,12 cis octadecadienoic acid［J］. Jaocs, 1970, 47: 242-244
［3］蔡燕, 石玉刚, 周红波, 等氢过氧化亚油酸钠在洗涤用品中的应用研究［J］. 日用化学工, 2012(43): 188-191(Cai Y, Shi Y, Zhou H B, et al Application of sodium linoleate hydroperoxide in laundry product［J］. China Surfactant Detergent & Cosmetics, 2012(43): 188-191)
［4］Rodrigo D, Jolie R, Van Loey A, et al Thermal and high pressure stability of tomato lipoxygenase and hydroperoxide lyase［J］. Journal of Food Engineering, 2007, 79: 423-429
［5］Indrawati, Amvan L, Ludikhuyze L R, et al Pressure temperature inactivation of lipoxygenase in green peas (Pisum sativum): A kinetic study［J］. Journal of Food Science, 2001, 66: 686-693
［6］Cai K, Fang Y, Xia Y M, et alEffect of exogenous iron on aerobic catalytic mechanism of soybean lipoxygenase［J］. Journal of Molecular Catalysis B: Enzymatic, 2004, 32:21-26
［7］蔡燕, 周红波, 鞠剑锋, 吴锦明外源钡离子对脂肪氧合酶好氧催化体系的影响［J］. 大豆科学, 2015, 34(6): 1053-1056(Cai Y, Zhou H B, Ju J F The effects of exogenous Ba2+on the lipoxygenase catalytic system［J］. Soybean Science, 2015, 34(6): 1053-1056)
［8］蔡琨, 方云, 夏咏梅, 等大豆脂肪氧合酶的提取及影响酶活因素的研究［J］. 林产化学与工业, 2004, 24(2): 10-15 (Cai K, Fang Y, Xia Y M, et al Extraction of soybean lipoxygenase and factors affecting its enzyme activity［J］. Chemical & Industry of Forest Products, 2004, 24(2): 10-15)
［9］Surrey K Spectrophotometric method for determination of lipoxygenase activity［J］. Plant Physiology, 1964, 65-70
［10］Gay C, Collins J, Gebicki J M Hydroperoxide assay with the ferric xylenol orange complex［J］. Analytical Biochemistry, 1999, 273: 149-155
［11］Jiang Z Y, Woolland A C S, Wolf S PLipid hydroperoxide measurement by oxidation of Fe2+in the presence of xylenol orang Comparison with the TBA assay and an iodometric method［J］. Lipids, 1991, 26: 853-856
［12］Cai Y, Xu H, Liu Y J, et al Soymeal catalyzed economic facile bioproduction of soybean oil hydroperoxide［J］. Biocatal Biotransform, 2011, 29(1): 31-36

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更新日期/Last Update: 2017-05-14