[1]高凤鸣,胡文婷,苏国兴.多胺诱导大豆子叶节愈伤组织形成丛生芽的机理研究[J].大豆科学,2015,34(06):1006-1010.[doi:10.11861/j.issn.1000-9841.2015.06.1006]
　GAO Feng-ming,HU Wen-ting,SU Guo-xing.Study on the Mechanism of Clump Bud Generation Induced by Polyamines in Soybean Cotyledon Node Callus[J].Soybean Science,2015,34(06):1006-1010.[doi:10.11861/j.issn.1000-9841.2015.06.1006]
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多胺诱导大豆子叶节愈伤组织形成丛生芽的机理研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 1006-1010 栏目: 出版日期: 2015-12-25
Title:
Study on the Mechanism of Clump Bud Generation Induced by Polyamines in Soybean Cotyledon Node Callus
作者:
高凤鸣; 胡文婷; 苏国兴
苏州大学 基础医学与生物科学学院，江苏 苏州 215123
Author(s):
GAO Feng-ming;  HU Wen-ting;  SU Guo-xing
School of Basic and Biological Science, Soochow University, Suzhou 215123, China
关键词:
大豆子叶节; 过氧化氢; 多胺氧化降解; 丛生芽
Keywords:
Soybean cotyledon node;  Polyamines oxidative degradation;  Hydrogen peroxide;  Clump buds
DOI:
10.11861/j.issn.1000-9841.2015.06.1006
文献标志码:
A
摘要:
以栽培大豆幼苗子叶节愈伤组织为材料，研究多胺氧化降解产物H2O2在大豆子叶节愈伤组织丛生芽形成中的作用。结果表明：浓度为0.5 mmol.L-1时3种外源多胺（Put, Spm和Spd）都能显著促进大豆子叶节愈伤组织分化形成丛生芽，其中Spd的作用最显著，Put和Spm作用次之，而亚精胺合酶抑制剂-环己胺（CHA）对大豆子叶节丛生芽的形成有较强的抑制作用。与此对应的是，外源多胺处理促进了子叶节外植体的二胺氧化酶（CuAO）活性和H2O2含量水平。有趣的是，用多胺氧化酶抑制剂-氨基胍（AG）和H2O2清除剂DMTU分别处理，也可显著抑制多胺对大豆子叶节愈伤组织分化形成丛生芽的促进作用； AG和DMTU 在丛生芽分化上的作用，可能与AG处理导致外植体CuAO活性和H2O2水平的降低，以及与DMTU清除H2O2的作用有关。研究结果暗示多胺降解产物H2O2可能参与多胺对大豆子叶节愈伤组织丛生芽的诱导。
Abstract:
Cotyledon node callus of soybean seedlings was used to study the role of hydrogen peroxide from polyamine degradation in induced generation of clump buds in soybean cotyledon node callus.The results showed that three of the exogenous polyamines significantly enhanced clump buds formation of soybean cotyledon node callus, 0.5 mmol?L-1Put was more effective than Spm, but less than Spd. Meanwhile cyclohexylamine (CHA), an inhibitor of Spd synthase, displayed a strong inhibitory effect on the formation of clump buds. Accordingly, activity of diamine oxidase and levels of hydrogen peroxide in the explants of cotyledon node were greatly increased. Interestingly, polyamine-increased differentiation of clump buds was strongly reduced after the application of diamine oxidase inhibitor-aminoguanidine (AG) and H2O2?scavenger ?dimethyl thiourea (DMTU), respectively.Probably, the effects of AG and DMTU on clump buds differentiation were associated with decreases in CuAO activity and the level of H2O2?caused by AG treatments, and H2O2scavenging by DMTU. These results suggest that hydrogen peroxide from polyamine degradation is potentially involved in inductive formation of clump buds in soybean cotyledon node callus.

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备注/Memo

基金项目：国家自然科学基金（31171467）。

第一作者简介：高凤鸣（1989-），女，硕士，主要从事多胺分解代谢在植物生长发育中的研究。E-mail:229525881@qq.com。
通讯作者：：苏国兴（1962-），男，副教授，硕导，主要从事植物细胞信号转导研究。E-mail:Suguoxing@suda.edu.cn。

更新日期/Last Update: 2016-01-06