[1]王伟,姚嘉龙,熊鹤雯,等.大豆科丰14子叶节遗传转化体系的优化[J].大豆科学,2015,34(03):389-393.[doi:10.11861/j.issn.1000-9841.2015.03.0389]
　WANG Wei,YAO Jia-long,XIONG He-wen,et al.Optimization on Cotyledonary-node Genetic Transformation System of Soybean Cultivar Kefeng 14[J].Soybean Science,2015,34(03):389-393.[doi:10.11861/j.issn.1000-9841.2015.03.0389]
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大豆科丰14子叶节遗传转化体系的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 389-393 栏目: 出版日期: 2015-06-25
Title:
Optimization on Cotyledonary-node Genetic Transformation System of Soybean Cultivar Kefeng 14
作者:
王伟; 姚嘉龙; 熊鹤雯; 吴志慧; 王程; 郭蓓; 谢皓
北京农学院 植物科学技术学院/北京农业应用新技术北京市重点实验室，北京 102206
Author(s):
WANG Wei; YAO Jia-long; XIONG He-wen; WU Zhi-hui;  WANG Cheng; GUO Bei; XIE Hao
College of Plant Science and Technology, Beijing University of Agriculture/ Beijing Key Laboratory of New Technology in Agricultural Application, Beijing 102206, China
关键词:
科丰14; 子叶节; 遗传转化
Keywords:
Kefeng 14;  Cotyledonary node;  Transformation
DOI:
10.11861/j.issn.1000-9841.2015.03.0389
文献标志码:
A
摘要:
科丰14是一个适应性较广的大粒型大豆品种，以农杆菌介导的子叶节法为转化途径，研究农杆菌侵染时间、6-BA浓度及培养基中添加硫代硫酸钠对转化率的影响，以建立适宜科丰14的子叶节遗传转化体系。结果表明：当农杆菌侵染外植体时间为15 min、丛生芽培养基中6-BA的浓度为1.50 mg?L-1时产生的丛生芽率最高，可达72.92%；在共培养基中加入1 mmol?L-1的硫代硫酸钠能有效防止外植体褐化，提高丛生芽率；利用-GUS-基因染色技术检测转化效果，表明在大豆常规转化体系的基础上，上述措施可提高科丰14的转化效率。
Abstract:
Kefeng 14 is a big grain type soybean cultivar with a wide adaptability.Based on the genetic transformation system of soybean cotyledonary node mediated by Agrobacterium tumefacines, the influences of the transformation efficiency were analyzed by infection time, 6-BA concentration and the addition of sodium thiosulfate.The results showed that bud rate was up to 72.92% with the optimal infection combination：A tumefacines- concetration at OD600=0.6 with 15 min infection time.The perfect 6-BA and sodium thiosulfate concentration was 1.50 mg?L-1and 1 mmol?L-1?respectively，GUS gene staining technique showed that the above optimized soybean cotyledonary node Agrobacterium-mediated transformation system could improve the transformation efficiency of Kefeng 14.

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备注/Memo

基金项目：国家自然科学基金(31371648);国家高技术研究发展计划“863计划”（2012AA101106）。

第一作者简介：王伟(1984-)，女，硕士，主要从事大豆遗传育种研究。E-mail:wangwei_wang@126.com。
通讯作者：谢皓（1962-），男，博士，教授，主要从事大豆遗传育种研究。E-mail:xiehao126@126.com。

更新日期/Last Update: 2015-07-15