[1]孙 昕,赵健如,闫 帆,等.吉林47大豆子叶节遗传转化体系的优化[J].大豆科学,2013,32(03):291-294.[doi:10.11861/j.issn.1000-9841.2013.03.0291]
　SUN Xin,ZHAO Jian-ru,YAN Fan,et al.Optimization on Cotyledonarynode Genetic Transformation System of Soybean Cultivar Jilin 47[J].Soybean Science,2013,32(03):291-294.[doi:10.11861/j.issn.1000-9841.2013.03.0291]
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吉林47大豆子叶节遗传转化体系的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年03期 页码: 291-294 栏目: 出版日期: 2013-06-25
Title:
Optimization on Cotyledonarynode Genetic Transformation System of Soybean Cultivar Jilin 47
作者:
?孙 昕; 赵健如; 闫 帆; 姬长媛; 杨旭光; 李景文; 王 英; 王庆钰
?吉林大学 植物科学学院,吉林 长春 130062
Author(s):
?SUN Xin; ZHAO Jian-ru; YAN Fan; JI Chang-yuan; YANG Xu-guang; LI Jing-wen; WANG Ying; WANG Qing-yu
?Plant Science College of Jilin University,Changchun 130062,China
关键词:
吉林47; 子叶节; 褐化; 芽伸长
Keywords:
Jilin 47; Cotyledonarynode; Browning; Shoot elongation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0291
文献标志码:
A
摘要:
?为检测不同基因型大豆对于根癌农杆菌的敏感性，使用携带pCAMBIA1301质粒（含GUS基因）的农杆菌EHA105对10个性状优良的大豆基因型进行侵染，共培养后进行GUS染色，筛选得到对根癌农杆菌较敏感的基因型吉林47、吉林35和绥农10号；而后以吉林47子叶节为外植体进行大豆遗传转化体系的优化，在重悬及共培养期间添加不同浓度巯基化合物，发现400 mg·L-1L-Cys、248 mg·L-1硫代硫酸钠和300 mg·L-1DTT组合对于褐化的抑制效果最好；对芽伸长阶段的关键激素GA3和IAA进行比较研究，得到最佳配比为1.0 mg·L-1GA3、0.5 mg·L-1IAA。
Abstract:
?After infected by EHA105 caring plasmid pCAMBIA1301,the GUS staining of 10 soybean genotypes was carried out to detect the sensitivity for Agrobacterium tumefaciens.Jilin 47，Jilin 35 and Suinong 10 were screened out as sensitive genenotypes.Jilin 47 was chosen as the genotype for optimization of soybean cotyledonary genetic transformation due to its good performance on shoot regeneration.Amending thiol compounds in liquid and solid cocultivation medium could decrease the browning rate of explants and the best combination of 400?mg·L-1 L-Cys, 248 mg·L-1 sodium thiosulfate and 300 mg·L-1DTT were obtained through orthogonal test.The highest elongation rate was got when 1.0 mg·L-1 GA3 and 0.5 mg·L-1IAA were added to shoot elongation medium.

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更新日期/Last Update: 2014-03-04