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[1]李文亮,边鸣镝,王海波.高产纳豆激酶突变菌株产酶条件的研究[J].大豆科学,2010,29(04):692-695.[doi:10.11861/j.issn.1000-9841.2010.04.0692]
　LI Wen-liang,BIAN Ming-di,WANG Hai-bo.Optimation on Fermentation Conditions of a High-yield Nattokinase Mutant[J].Soybean Science,2010,29(04):692-695.[doi:10.11861/j.issn.1000-9841.2010.04.0692]

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高产纳豆激酶突变菌株产酶条件的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷期数: 2010年04期页码: 692-695 栏目: 出版日期: 2010-08-25

Title:: Optimation on Fermentation Conditions of a High-yield Nattokinase Mutant

文章编号:: 1000-9841（2010）04-0692-04

作者:: 李文亮; 边鸣镝; 王海波; 吉林大学农学部军需科技学院，吉林长春 130062

Author(s):: LI Wen-liang; BIAN Ming-di; WANG Hai-bo; College of Quartermaster Technology,Department of Agronomy,Jilin University,Changchun 130062，Jilin，China

关键词:: 纳豆; 纳豆激酶; 纤维蛋白平板法; 条件优化

Keywords:: Natto; Nattokinase; Fibrin plate; Conditions optimization

分类号:: TQ464.8

DOI:: 10.11861/j.issn.1000-9841.2010.04.0692

文献标志码:: A

摘要:: 为了获得最佳产酶条件，通过研究不同碳源（葡萄糖、木糖、麦芽糖、蔗糖、淀粉），氮源（牛肉膏、蛋白胨、大豆蛋白胨、酵母膏、硫酸铵），碳氮浓度比和无机离子组成的培养基发酵，利用纤维蛋白平板法测定纳豆激酶的活性。得到纳豆激酶的最佳产酶条件为：1.5%蛋白胨，1.5%麦芽糖，0.05%硫酸镁，0.2%氯化钙，0.2%磷酸二氢钠和0.1%磷酸氢二钠；碳氮浓度比为1∶1。

Abstract:: In order to obtain optimal conditions for nattokinase production，the influencing factors of carbon source（glucose，sucrose）,nitrogen source（beef extract，peptone，soy peptone，yeast extract，ammonia sulfate），C/N concentration and inorganic ions composition were investigated by fermentation culture and the activity of nattokinase was determined by fibrin plate method. Results showed that the best condition of enzyme production was 1.5% peptone，1.5% maltose，0.05% MgSO₄，0.2%CaCl₂，0.2%NaH₂PO₄ and 0.1%Na₂HPO₄,with the carbon and nitrogen concentration ratio of 1∶1.

参考文献/References:

[1]Fujita M，Nomura K，Hong K，et al.Purification and characterization of a strong fibrinolytic enzyme(nattokinase)in the vegetable cheese natto，a popular soybean fermented food in Japan[J]．Biochemical and Biophysical Research Communications，1993，197(3)：1340-1347.

[2]Deepak V， Kalishwaralal K， Ramkumarpandian S，et al.Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology[J].Bioresource Technology，2008,99：8170-8174.

[3]陈启和，何国庆．纳豆激酶的研究进展[J].食品与发酵工业，2001，27（12）：55-58.（Chen Q H,He Guo Q.Review of Nattokinase research[J].Food and Fermentation Industries,2001,27(12):55-58.）

[4]朱健辉，杜连祥，路福平，等.高产纳豆激酶液态发酵工艺的优化[J].工业微生物，2007，37（1）：20-24.(Zhu J H,Du L X,Lu F P,et al.Optimization of fermentation conditions for high-production nattokinase[J].Industrial Microbiology,2007,37(1):20-24.)

[5]熊晓辉，李睿，陆利霞，等.纳豆激酶的分离纯化及其特性研究[J].食品与发酵工业，2006，32（1）：122-125.(Xiong X H,Li R,Lu L X，et al.Study on the purification of Nattokinase from the liquid fermentation and its characteristics[J].Food and Fermentation Industries,2006,32(1):122-125.)

[6]杨郁，张丽靖，天知诚吾.纳豆激酶高产菌株筛选及发酵条件优化[J].现代食品科技，2007，23（10）：22-25.(Yang Y,Zhang L J,Seigo Amachi.Selection of high Nattokinase-producing strains and optimization of the fermentation conditions[J].Modern Food Science and Technology,2007,23(10):22-25.)

[7]方祥，周换彩，王忠霞，等.纳豆菌分离、鉴定及纳豆激酶高产菌株的正向选育[J].食品与发酵工业，2005，31（12）：26-29.(Fang X,Zhou H C,Wang Z X ,et al.Isolation and forward breeding of bacillus subtilis Natto with high fibrinolytic activity Nattokinase[J].Food and Fermentation Industries,2005,31(12):26-29.)

[8]黄薇，孔繁东，祖国仁，等.高NK活性纳豆菌的诱变选育及产酶条件的研究[J].食品科技，2007(4)：17-21.(Huang W,Kong F D,Zu G R,et al.High NK activity of bacillus natto by mutation breeding and study on culture conditions of nattokinase production[J].Food Science and Technology,2007(4):17-21.)

[9]余功保，郭爱玲，秦巧玲，等.一株高产纳豆激酶菌株的筛选及其发酵条件的优化[J].食品科学，2007，28（4）：227-231.(Yu G B,Guo A L,Qin Q L,et al.Screening of high Nattokinase activity producing strain and optimization of fermentation conditions[J].Food Science，2007，28（4）：227-231.)

[10]张谦，陈大钧，熊颖，等.纳豆激酶液体发酵条件的研究[J].四川食品与发酵，2007（5）：42-46.（Zhang Q，Chen D J，Xiong Y，et al.Study on the liquid fermentation of Nattokinase[J].Sichuan Food and Fermentation，2007（5）：42-46.）

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备注/Memo

基金项目：吉林大学农学部青年基金资助项目（4305050102E7）。

第一作者简介：李文亮（1969-），男，讲师，硕士，现主要从事微生物发酵研究。E-mail：liwenliang2001@yahoo.com.cn。

更新日期/Last Update: 2014-09-15