[1]陈井生,陈立杰,刘大伟,等.放线菌Snea49的种类鉴定及对胞囊线虫的活性评价[J].大豆科学,2010,29(04):663-665.[doi:10.11861/j.issn.1000-9841.2010.04.0663]
　CHEN Jing-sheng,CHEN Li-jie,LIU Da-wei,et al.Indentification of the Strain Snea49 and Evaluation for Nematicidal Activity Against Soybean Cyst Nematode[J].Soybean Science,2010,29(04):663-665.[doi:10.11861/j.issn.1000-9841.2010.04.0663]
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放线菌Snea49的种类鉴定及对胞囊线虫的活性评价

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年04期 页码: 663-665 栏目: 出版日期: 2010-08-25
Title:
Indentification of the Strain Snea49 and Evaluation for Nematicidal Activity Against Soybean Cyst Nematode
文章编号:
1000-9841（2010）04-0663-03
作者:
陈井生1; 2; 陈立杰1; 刘大伟1; 朱晓峰1; 王媛媛1; 段玉玺1
1.沈阳农业大学 植物保护学院，辽宁 沈阳 110161；
2.黑龙江省农业科学院 大庆分院，黑龙江 大庆，163316
Author(s):
CHEN Jing-sheng1; 2; CHEN Li-jie1; LIU Da-wei1; ZHU Xiao-feng1; WANG Yuan-yuan1; DUAN Yu-xi1
1.Plant Nematology Laboratory, Shenyang Agricultural University, Shenyang 110161,Liaoning；
2.Daqing Branch, Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China
关键词:
放线菌; 大豆胞囊线虫; 16S rDNA; 2龄幼虫
Keywords:
Actinomycetes; Heterodera glycines; 16S rDNA; second stage juvenile （J2）
分类号:
S154.38
DOI:
10.11861/j.issn.1000-9841.2010.04.0663
文献标志码:
A
摘要:
采用稀释分离法从辽宁沈阳市土壤中分离到1株放线菌Snea49。对Snea49进行了形态学特征、培养特征、生理生化特征及16S rDNA分析，初步鉴定为环状链霉菌(Streptomyces anulatus)。利用离体测试法，研究了Snea49菌株代谢物不同倍数稀释液对大豆胞囊线虫的抑制作用。结果表明：10倍稀释液浓度下,该菌株对胞囊孵化的相对抑制率达82.60%，与无菌水对照差异显著。处理24 h后,在1倍稀释液浓度下, 该菌株对2龄幼虫的校正死亡率是89.66%,各稀释浓度均与无菌水对照差异显著，该菌株有较高的杀线活性。
Abstract:
Strain Snea49，was isolated by diluted solution from a soil sample collected in Liaoning province.The results based on characteristics of morphological，physiology and biochemistry and 16S rDNA sequence analysis of strain Snea49 showed that it was Streptomyces anulatus. Fermentation filtrates of Streptomyces anulatus at different concentrations were evaluated for nematicidal activity against soybean cyst nematode in the laboratory，bioassays included egg hatching and mortality of second stage juvenile.The hatching rate of cysts were suppressed by 82.60% at 10× diluted solution. The corrected mortality rates of juveniles were 89.66% when J2 were treated with the strain at 1× dilution for 24 hours. The difference between these treatments and water control were significant. Fermentation filtrates of the strains were effective in inhibiting H. glycines?hatch and killing J2.

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备注/Memo

基金项目：教育部新世纪优秀人才支持计划资助项目(NCET-08-0866)；辽宁省优秀人才支持计划资助项目(RC-05-18)。

第一作者简介：陈井生（1982-）,男，硕士,研究实习员,研究方向为大豆胞囊线虫抗性育种和生物防治。E-mail:chenjingsheng1982@hotmail.com。
通讯作者：陈立杰,教授,博士生导师。 E-mail:chenlj@syau.edu.cn。

更新日期/Last Update: 2014-09-14