[1]陈乃松,杨志刚,崔惟东,等.酶制剂体外酶解豆粕中抗营养因子的研究[J].大豆科学,2008,27(04):663-668.[doi:10.11861/j.issn.1000-9841.2008.04.0663]
　CHEN Nai-song,YANG Zhi-gang,CUI Wei-dong,et al.Enzymes Hydrolyzing in Vitro Anti-Nutritional Factors in Soybean Meal[J].Soybean Science,2008,27(04):663-668.[doi:10.11861/j.issn.1000-9841.2008.04.0663]
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酶制剂体外酶解豆粕中抗营养因子的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 663-668 栏目: 出版日期: 2008-08-25
Title:
Enzymes Hydrolyzing in Vitro Anti-Nutritional Factors in Soybean Meal
文章编号:
1000-9841(2008)04-0663-06
作者:
陈乃松; 杨志刚; 崔惟东; 周洁; 马建忠; 王战付; 郑剑伟
上海水产大学生命科学与技术学院，上海 200090
Author(s):
CHEN Nai-song; YANG Zhi-gang; CUI Wei-dong; ZHOU Jie; MA Jian-zhong; WANG Zhan-fu; ZHENG Jian-wei
College of Aqua-life Science and Technology，Shanghai Fisheries University，Shanghai 200090，China
关键词:
酶制剂; 降解; 豆粕; 抗营养因子
Keywords:
Enzyme preparation; Degradation; Soybean meal; Anti-nutritional factors
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0663
文献标志码:
A
摘要:
为了评价几种单一酶、复合酶以及钙离子对豆粕中的相关抗营养因子的水解效果，进行了体外酶解试验。共有4种单一酶制剂（植酸酶、果胶酶、纤维素酶和木聚糖酶）和3种复合酶制剂被采用，其中复合酶SB由α-半乳糖苷酶和木聚糖酶组成，复合酶C3由植酸酶、果胶酶、纤维素酶组成，复合酶C5由植酸酶、果胶酶、纤维素酶、木聚糖酶和α-半乳糖苷酶组成。预试验显示上述酶有基本相似的最适水解反应参数：水分50%、pH 4.8~5.0、温度50℃和时间45 min。在此参数下，添加10.5 Ug-1豆粕的植酸酶，豆粕中植酸的降解率达到69.63%；添加果胶酶16 U.g-1豆粕，豆粕中果胶的降解率达到45.93%；添加纤维素酶50 U. g-1豆粕，纤维素降解率达到66.21%；分别添加木聚糖酶80 U.g-1豆粕和α-半乳糖苷酶0.7 U.g-1豆粕，豆粕中还原糖的释放量增加率分别达到84.77%和65.19%。在豆粕中添加0.2% CaCl2，植酸的降解率与对照组相比微增至71.09%，还原糖的释放量分别增加到88.68%和65.23%。但果胶和纤维素的降解率分别显著地增加到90.01%和70.43%。研究发现复合酶C3和C5对相关的抗营养因子均呈现出不同程度的协同效应。说明使用复合酶制剂并适量添加CaCl2，体外水解豆粕中的相关抗营养因子是可行的。
Abstract:
In vitro experiments with several enzyme preparations were conducted to evaluate the effects of several kinds of single enzyme and compound enzyme and Ca2+on hydrolyzation of relative anti-nutritional factors (ANFs) in soybean meal (SBM).Four single enzymes (phytase，pectase，cellulase，xylanase) and three enzyme compounds (SB-including α-galactosidase and xylanase，C3 including phytase，pectase and cellulase，C5 including phytase，pectase，cellulase，xylanase and SB) were employed.A beforehand test showed that the suitable hydrolytical parameter for these enzymes was similarly 50% for moisture，4.8-5.0 for pH，50℃ for incubation temperature，45 minutes for incubation time.With the parameter and addition of 10.5 U phytase in 1 g SBM，the degradation rate of phytate reached 69.63%.The degradation rates of pectin and cellulose were 45.93% and 66.21% with adding 16 U pectase and 40 U cellulase in 1 g SBM respectively，and with adding 80 U xylanase and 0.7 U a-galactosidase in 1 g SBM，the release rates of reducing sugar increased to 84.77% and 65.19% respectively.With 0.2% CaCl2 supplementation in SBM，the degradation rate of phytate reached 71.09%，slightly higher than 69.63% for the control and the release rates of reducing sugar increased to 88.68% and 65.23% respectively，but the degradation rates of pectin and cellulose increased significantly to 90.01% and 70.43%.Synergistic effects on phytate，pectin and cellulose degradation as well as release of reducing sugars were observed when either C3 or C5 was used.This research indicated that multi-enzyme preparation with moderate CaCl2 supplementation was feasible to hydrolyze in vitro specific ANFs in SBM.

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备注/Memo

基金项目：上海市教委资助项目(科-05227)；上海市农委攻关资助项目(攻-0664)。

作者简介：陈乃松(1961-)，男，在读博士，副教授，从事水产动物营养与饲料学研究。E-mail: nschen@shfu.edu.cn。

更新日期/Last Update: 2014-10-07