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[1]何喜珍,牛延宁,金明飞,等.不同分子量大豆多糖的表征和抗氧化研究[J].大豆科学,2016,35(05):805-809.[doi:10.11861/j.issn.1000-9841.2016.05.0805]
　HE Xi-zhen,NIU Yan-ning,JIN Ming-fei,et al.Characterization and Antioxidation Study of Different Molecular Weight of Soluble Soybean Polysaccharides[J].Soybean Science,2016,35(05):805-809.[doi:10.11861/j.issn.1000-9841.2016.05.0805]

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不同分子量大豆多糖的表征和抗氧化研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷期数: 2016年05期页码: 805-809 栏目: 出版日期: 2016-09-20

Title:: Characterization and Antioxidation Study of Different Molecular Weight of Soluble Soybean Polysaccharides

作者:: 何喜珍¹; 牛延宁¹; 金明飞¹; 黄静¹; 崔红亮²; 常忠义¹; 高红亮¹; （1.华东师范大学生命科学学院，上海 200241； 2. 平顶山金晶生物科技有限公司，河南平顶山 467215）

Author(s):: HE Xi-zhen¹; NIU Yan-ning¹; JIN Ming-fei¹; HUANG Jing¹; CUI Hong-liang²; CHANG Zhong-yi¹; GAO Hong-liang¹; （1.School of Life Science，East China Normal University，Shanghai 200241, China； 2.Pingdingshan Jinjing Biotechnology Company Limited，Pingdingshan 467215, China）

关键词:: 大豆多糖; 降解; 抗氧化; 表征

Keywords:: Soluble soybean polysaccharides; Degration; Antioxidant; Characterization

DOI:: 10.11861/j.issn.1000-9841.2016.05.0805

文献标志码:: A

摘要:: 以酸提大豆多糖为原料，通过控制降解条件得到4种不同分子量的大豆多糖样品，分子量分别为550，347，285和21 kDa。对4种多糖进行了傅里叶红外（FT-IR）分析和X射线衍射（XRD）分析，并分别测定了4种多糖对DPPH自由基、羟基自由基的清除能力以及还原能力的大小，结果表明：4种大豆多糖都有一定的抗氧化活性，但抗氧化活性高低有所不同，分子量为285 kDa的大豆多糖对自由基的清除能力最强，还原能力最强，分子量为21 kDa的大豆多糖对自由基的清除能力最弱，还原能力最弱。

Abstract:: Four different molecular-weight soluble soybean polysaccharides were prepared by controlling the degration condition, with the molecular weights 550, 347, 285 and 21 kDa, respectively. The structures of them were identified by Fourier transform infrared spectrometer (FTIR) and X.Ray Diffraction (XRD) Antioxidant activities of four SSPS were evaluated in vitro by DPPH free radical scavenging capacity, hydroxyl free radical scavenging and total reducing power. The results showed that all of them had antioxidant activities. The SSPS-H with molecular weight of 285 kDa had the strongest antioxidant activities in all antioxidant systems, while the SSPS-CH with molecular weight of 21 kDa performed the worst.

参考文献/References:

［1］Furuta H, Takahashi T, Tobe J, et al. Extraction of water-soluble soybean polysaccharides under acidic conditions［J］. Bioscience Biotechnology and Biochemistry, 1998, 62(12): 2300-2305.

［2］Furuta H, Tobe J, Kiwata R, et al. Rheological properties of a heat-reversible gel of water-soluble-soybean polysaccharide extracted under acidic condition［J］. Bioscience Biotechnology and Biochemistry, 1999, 63(12): 2075-2081.?

［3］Furuta H, Maeda H. Rheological properties of water-soluble soybean polysaccharides extracted under weak acidic condition［J］. Food Hydrocolloids, 1999, 13(3): 267-274.?

［4］Nakamura A, Nobuhara T, Matsumiya K, et al. Stabilization of milk protein dispersion by soybean soluble polysaccharide under acidic pH conditions［J］. Food Hydrocolloids, 2014, 34(1):39-45.?

［5］Nakamura A, Maeda H, Corredig M. Emulsifying properties of enzyme-digested soybean soluble polysaccharide［J］. Food Hydrocolloids, 2006, 20(7): 1029-1038.?

［6］Nakamura A, Yoshida R, Maeda H, et al. Soy soluble polysaccharide stabilization at oil–water interfaces［J］. Food Hydrocolloids, 2006, 20(2): 277-283.?

［7］Aimin T, Hongwei Z, Gang C, et al. Influence of ultrasound treatment on accessibility and regioselective oxidation reactivity of cellulose［J］. Ultrasonics Sonochemistry, 2005, 12(6): 467-472.?

［8］Zha X, Wang J, Yang X, et al. Antioxidant properties of polysaccharide fractions with different molecular mass extracted with hot-water from rice bran［J］. Carbohydrate Polymers, 2009, 78(3): 570-575.?

［9］Im S, Oh S, Song S, et al. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity［J］. International Immunopharmacology, 2005, 5(2): 271-279.

［10］Christodouleas D C, Fotakis C, Papadopoulos K, et al.Evaluation of total reducing power of edible oils［J］.Talanta, 2014, 130(5): 233-240.

［11］Zhao X H. Some factors that affect the free radical scavenging activity of tea extracts［J］.Journal of Northeast Agricultural University, 1999, 5(1): 62-67.

［12］Zeng Y, Deng M, Lyu Z, et al.Evaluation of antioxidant activities of extracts from 19 Chinese edible flowers［J］. Springerplus, 2014, 3(1): 1-5.

［13］Wong C, Li H, Cheng K, et al. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay ［J］. Food Chemistry, 2006, 97(4): 705-711.

［14］尹艳.两种水溶性大豆多糖的红外光谱分析［J］. 食品研究与开发, 2013, 34(8): 67-68.(Yin Y. Study on the infrared spectrum analysis of two kinds of soluble soybean polysaccharides［J］.Food Research and Development, 2013, 34(8): 67-68.)

［15］苏浩. 豆渣中水溶性大豆多糖提取、结构鉴定及物性学研究［D］.乌鲁木齐：新疆农业大学, 2009: 83.(Su H.Study on extraction, structure identification and physical properties of water-soluble soybean polysaccharide from soybean dregs［D］.Urumuqi: Xinjiang Agricultural University, 2009:83.)

［16］Salarbashi D, Mortazavi S A, Noghabi M S, et al.Development of new active packaging film made from a soluble soybean polysaccharide incorporating ZnO nanoparticles ［J］. Carbohydrate Polymers, 2016, 140: 220-227.

［17］尹艳,高文宏. 两种水溶性大豆多糖的X.射线衍射分析［J］. 食品研究与开发, 2014, 35(6): 1-2.(Yin Y, Gao W H. Study on analyzing X.ray diffraction patterns of two kinds of soluble soybean polysaccharides［J］. Food Research and Development, 2014, 35(6): 1-2. )

［18］Halliwell B, Gutteridge J, Aruoma O. The deoxyribose method: A simple ‘test-tube’ assay for determination of rate constants for reactions of hydroxyl radicals ［J］. Analytical Biochemistry, 1987, 165(1): 215-219.

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备注/Memo

基金项目：国家自然科学基金（31170920）。第一作者简介：何喜珍（1989-），女，硕士，主要从事天然多糖应用研究。E.mail: hexizhen2010@163.com。通讯作者：高红亮（1972-），男，博士，副教授，主要从事食品生物技术研究。E-mail: hlgao@bio.ecnu.edu.cn。

更新日期/Last Update: 2016-09-25