[1]宋雯雯,刘宝辉,杨明亮,等.农杆菌介导法将 SPS 基因导入大豆的研究[J].大豆科学,2008,27(03):387-390.[doi:10.11861/j.issn.1000-9841.2008.03.0387]
　SONG Wen-wen,LIU Bao-hui,YANG Ming-liang,et al.Transformation of SPS Gene into Soybean via Agrobacterium-mediated Method[J].Soybean Science,2008,27(03):387-390.[doi:10.11861/j.issn.1000-9841.2008.03.0387]
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农杆菌介导法将 SPS 基因导入大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 387-390 栏目: 出版日期: 2008-06-25
Title:
Transformation of SPS Gene into Soybean via Agrobacterium-mediated Method
文章编号:
1000-9841(2008)03-0387-04
作者:
宋雯雯; 刘宝辉; 杨明亮; 张颖; 王继安
东北农业大学大豆研究所，教育部大豆生物学重点实验室，黑龙江 哈尔滨 150030
Author(s):
SONG Wen-wen; LIU Bao-hui; YANG Ming-liang; ZHANG Ying; WANG Ji-an
Soybean Research Institute，Northeast Agricultural University/Key Laboratory of National Educational Department，Harbin 150030，Heilongjiang,China
关键词:
大豆胚尖; 农杆菌介导; SPS基因
Keywords:
Embryonic tips of soybean; Agrobacterium-mediation; SPS gene
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0387
文献标志码:
A
摘要:
长期以来，大豆遗传转化由于受基因型限制，受体系统不完善、实验结果重复性差等因素，使农杆菌介导的大豆遗传转化频率低下。农杆菌介导法多以子叶节为受体系统，但转化体极易形成嵌合体，导致后期筛选难度加大。基因枪方法的技术难度大、易引起基因沉默。以大豆胚尖为受体，采用农杆菌介导法将玉米蔗糖磷酸合成酶(SPS)基因导入大豆基因组中。由于胚尖具有较强的分生能力，再生细胞由转化细胞而来，极大地降低了生成嵌合体的可能性。卡那霉素抗性植株经PCR 及Southern 杂交等分子检测，证明目的基因已导入并整合到大豆基因组中。
Abstract:
The low efficiency of soybean genetic transformation was contributed by restricted soybean genotypes as receptors，the faultiness of tissue culture system，etc.Through Agrobacterium-mediated transformation of cotyledonary node，the most of genetic plants were chimeras.Consequently，it was difficult to select genetic transformation plants.Another popularly used method-Particle Bombardment，whose technique was hard to operate，easily resulted in gene silence.In this research，SPS gene was transformed into embryonic tips of soybean via Agrobacterium-mediated method.The embryonic tip was primary meristem which originated from primordial meristem，therefore it had strong dividing capability.Regeneration cells were derived from genetic cells，which decreased the possibility of being chimeras.The regenerated plants，which were resistant to Kanamycin，were obtained and examined by PCR and Southern blot.The results indicated that the target gene had been transformed into soybean genome.

参考文献/References:

[1] Cheng T Y，Saka T，Voqui-Dinh T H.Plant regeneration from soybean cotyledonary node segments in culture[J].Plant Science Letters，1980，19：91-99.

[2]Donaldson P A，Simmonds D H.Susceptibility to Agtobacterium tumefaciens and cotyledonary node transformation in short-season soybean[J].Plant Cell Reports，19：478-484.
[3]Hinchee M A，Conner Ward D V，Newell C A，et al.Production of transgenic soybean plants using Agrobacterium-mediated DNA Transfer[J].Bio-technology，1988，6：915-922.
[4]McCabe D E，Swain W F，Martinell B J，et al.Stable transformation of soybean(Glycine max) by particle acceleration.Bio/Technology，1988，6：923-926.
[5]刘海坤，卫志明.大豆遗传转化研究进展[J].植物生理与分子生物学学报，2005，31(2)：126-134.(Liu H K，Wei Z M.Recent advances in soybean genetic transformation[J].Journal of Plant Physiology and Molecular Biology，2005，31(2)：126-134.)
[6]李文霞，宁海龙，李文滨.大豆遗传转化的研究进展[J].中国农学通报，2005，12(31)：67-71.(Li W X，Ning H L，Li W B.Research on progress of transgene system in soybean[J].Chinese Agricultural Science Bulletin，2005，12(31)：67-71.)
[7]Trick H N，Dinkins R D，Santarn E R，et al.Recent advances in soybean transformation[J].Plant Tiss Cult Biotechnol，1997，3：9-26.
[8]杨荣仲，谭裕模，陈如凯.大豆遗传转化研究进展[J].广西农业科学，2003，6：11-14.(Yang R Z，Tan Y M，Cheng R K.Recent advances in soybean genetic transformation[J].Guangxi Agricultural Sciences，2003，6：11-14.)
[9]刘海坤，卫志明.利用根癌农杆菌介导转化大豆成熟种子胚尖获得转基因植株[J].植物生理与分子生物学学报，2004，30(6)：631-636.(Liu H K，Wei Z M.Transgenic soybean obtained with agrobacterium tumfaciens-mediated transformation of embryonic tip of soybean mature seeds[J].Journal of Plant Physiology and Molecular Biology，2004，30(6)：631-636.)
[10]周平，叶冰莹，陈由强，等.蔗糖磷酸合成酶研究的新进展[J].生物技术通讯，2006，17(6)：1001-1003.(Zhou P，Ye B Y，Cheng Y Q，et al.The recent advances on sucrose phosphate synthase[J].Letters in Biotechnology，2006，17(6)：1001-1003.)
[11]李雯，邵远志，庄军平，等.蔗糖磷酸合成酶与香蕉果实成熟、衰老的关系[J].园艺学报，2006，33(5)：1087-1089.(Li W，Shao Y Z，Zhuang J P，et al.Relationships between the Sucrose phosphate synthase and ripening，senescence of banana fruits[J].Acta Horticulturae Sinica，2006，33(5)：1087-1089.)

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备注/Memo

基金项目：国家自然科学基金资助项目(30471092)。

作者简介：宋雯雯(1982-)，女，硕士研究生，研究方向为大豆生物技术。E-mail:songwenwen2002@163.com。

更新日期/Last Update: 2014-10-07