[1]赵印华,陈颖珊,郭丽琼,等.pCAAFP66表达载体的构建及其大豆遗传转化的研究[J].大豆科学,2011,30(04):541-545.[doi:10.11861/j.issn.1000-9841.2011.04.0541]
　ZHAO Yin-hua,CHEN Ying-shan,GUO Li-qiong,et al.Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(04):541-545.[doi:10.11861/j.issn.1000-9841.2011.04.0541]
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pCAAFP66表达载体的构建及其大豆遗传转化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年04期 页码: 541-545 栏目: 出版日期: 2011-08-25
Title:
Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method
文章编号:
1000-9841（2011）04-0541-05
作者:
赵印华1; 陈颖珊1; 郭丽琼1; 2; 覃凤云1; 林俊芳1; 2
1.华南农业大学 食品学院，广东 广州 510642；
?2.华南农业大学 生物质能研究所，广东 广州 510642
Author(s):
ZHAO Yin-hua1; CHEN Ying-shan1; GUO Li-qiong1; 2; QIN Feng-yun1; LIN Jun-fang1; 2
1.College of Food Science,South China Agricultural University,Guangzhou 510642；
?2.Institute of Biomass Research,South China Agricultural University,Guangzhou 510642,Guangdong,China
关键词:
抗冷冻蛋白; 载体构建; 农杆菌介导; 大豆遗传转化
Keywords:
afp gene; Vector construction; Agrobacterium-mediated; Soybean genetic transformation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0541
文献标志码:
A
摘要:
以pCAMBIA3301为载体骨架，afp为目的基因，构建了大小为9 868 bp的大豆表达载体pCAAFP66，该载体带有抗冷冻蛋白基因afp和筛选标记基因bar。以大豆品种华春3号和华春6号的胚尖和子叶节为外植体，应用农杆菌介导法进行遗传转化，以草甘膦(PPT)为筛选底物。结果表明：在胚尖转化体系中，华春3号和华春6号初转化频率分别为7.0%和4.0%，在子叶节转化体系中，2个品种都未获得转化植株。
Abstract:
Plant expression vector pCAAFP66(9868 bp),which contained the target gene afp and the screening marker Bar gene,was constructed for soybean transformation.The anti-freezing gene afp was transformed into embryonic tip and cotyledonary node of soybean using Agrobacterium-mediated method and PPT as selective agent.Identification indicated the afpgene was successfully transformed into Huachun 3 and Huachun 6 through the embryonic tip transformation system,with transformation rates 7.4% and 4.0%, respectively.Whereas in the cotyledonary node system no transformed plants was obtained.Analysis of the embryonic tip and cotyledonary node approach revealed that significant differences existed in the regeneration and transformation rate between two genotypes;even with the same genotype,different explants materials had totally different transformation rates.

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项资助项目（2009ZX08004-007B）；广东省科技计划资助项目（2009A020102004）；华南农业大学“211工程”三期资助项目（2009C010500001）。

第一作者简介：赵印华（1984-），男，在读硕士，研究方向为大豆转基因育种。E-mail:zhaoyinhua110@yahoo.com.cn。
通讯作者：林俊芳(1962-），男，博士生导师，主要从事生物活性物质与生物炼制方面的研究。E-mail：linjf@scau.edu.cn。

更新日期/Last Update: 2014-08-15