[1]王涛,秦贵信,赵元,等.大豆皮中大豆球蛋白、β-伴大豆球蛋白和凝集素免疫活性的检测[J].大豆科学,2008,27(01):158-161.[doi:10.11861/j.issn.1000-9841.2008.01.0158]
　WANG Tao,QIN Gui-xin,ZHAO Yuan,et al.Determination of Immunocompetence of Glycinin β-conglycinin and Soybean Agglutinin in Soybean Hulls[J].Soybean Science,2008,27(01):158-161.[doi:10.11861/j.issn.1000-9841.2008.01.0158]
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大豆皮中大豆球蛋白、β-伴大豆球蛋白和凝集素免疫活性的检测

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 158-161 栏目: 出版日期: 2008-02-25
Title:
Determination of Immunocompetence of Glycinin β-conglycinin and Soybean Agglutinin in Soybean Hulls
作者:
王涛; 秦贵信; 赵元; 孙泽威; 王利民; 鲍男; 张兵; 朱丹; 孙玲; 王合峰
吉林农业大学动物科技学院，吉林长春 130118
Author(s):
WANG Tao; QIN Gui-xin; ZHAO Yuan; SUN Ze-wei; WANG Li-min; BAO Nan; ZHANG Bing; ZHU Dan; SUN Ling; and WANG He-feng
College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，Jilin，China
关键词:
大豆皮; 大豆球蛋白; β-伴大豆球蛋白; 凝集素; 竞争ELISA法
Keywords:
Soybean hulls; Glycinin; β-conglycinin; Soybean agglutinin; Inhibition ELISA
DOI:
10.11861/j.issn.1000-9841.2008.01.0158
文献标志码:
A
摘要:
由于大豆皮具有多种有益的功能，因而被添加于多种动物的饲料中。国内外学者对大豆皮的研究主要集中在大豆皮的可消化性和能量价值，而对大豆皮中抗营养因子组成及含量的研究报告非常的少。本文用竞争ELISA法对大豆皮中具有免疫活性大豆球蛋白、β-伴大豆球蛋白和凝集素的含量进行了测定，结果显示生大豆皮中具有免疫活性的大豆球蛋白、β-伴大豆球蛋白和凝集素的含量分别为25.3、41.4和12.2 mg g-1，说明大豆皮中含有相当高水平的抗营养因子。因此，大豆皮添加到动物饲料中之前，必须经过适当的加工处理，以在最大程度上灭活其中具有免疫活性的抗营养因子，且应该注意添加量等问题。
Abstract:
Soybean hull is added to the diet for its multifunctional characteristics.Researches of soybean hulls mostly focused on its digestibility and energy value，little reports were found on their content of antinutritional factors.The immunocompetence of glycinin，β-conglycinin and soybean agglutinin in soybean hulls were determined by the inhibition ELISA in this article；Results showed that the immunocompetence of glycinin，β-conglycinin and soybean agglutinin in soybean hulls was 25.3，41.4，and 12.2 mg g-1，respectively，which showed that the immunocompetence of glycinin，β-conglycinin and soybean agglutinin in soybean hulls was quite high.So it is necessary to process the soybean hulls in order to make the immunocompetence of glycinin，β-conglycinin and soybean agglutinin in soybean hulls inactive before adding it to the diet，and more attention should be paid on the additive quantity.

参考文献/References:

[1]Barratt M E J，Strachan P J，and Porter P.1979.Immunologically mediated nutritional disturbances associated with soyaprotein antigens.Proceedings of the Nutrition Society，38：143-150

[2]Cole J T，Fahey G C，Merchen N R，Patil A R，Murray S M，Hussein H S，and Brent J L.1999.Soybean hulls as a dietary fiber source for dogs.Journal of Animal Science，77：917-924?
[3]Cunningham K D，Cecava M J，and Johnson.T R.1993.Nutrient digestion，nitrogen，and amino acid flows in lactating cows feedsoybean hulls in place of forage or concentrate.Journal of Dairy Science，76:3523-3535
[4]He Z Y，Hu G X，and Wang C F，eds.2005.Animal immunological technology.Jilin Science Techlogy Press，China，Changchun，pp.71-76（何昭阳，胡桂学，王春凤，著.2005.动物免疫学实验技术.吉林科学技术出版社，中国，长春，pp.71-76）
[5]Iwabuchi S，and Yamauchi F.1987.Determination of glycinin and β-conglycinin in soybean protein by immunological methods.Journal of Agricultural and Food Chemistry，35:200-205
[6]Jin J，Zhao X，and Zhang Y.2003.Purification of Soybean Agglutinin and the Investigation of its Agglutination of different Tumor Cells.Journal of China Biological Chemistry and Molecular Biology，19(3)：401-405.（荆剑，赵翔，张页.2003.大豆凝集素的纯化及其凝集不同肿瘤细胞的探讨.中国生物化学与分子生物学报，19（3）：401-405）
[7]Kornegay E T.1981.Soybean hull digestibitity by sows and feeding value for growing finishing swine.Journal of Animal Science，53：138-145
[8]Li D F，ed.2003.Soybean Antinutritional Factors.China Science Press，China，Beijing，pp.154（李德法，著.2003.大豆抗营养因子.中国科学技术出版社，中国，北京，pp.154）
[9]Li Z T.2003.Determination，Purification of Soybean Agglutinin and the Investigation of its Antinutritional Mechanism on Rat.Dissertation for Ph D，College of Animal Science and Technology of China Agricultural University，Supervisor：Li D F，pp.20-28(李振田.2003.大豆凝集素的检测/纯化和对大鼠抗营养机理的研究.博士学位论文.中国农业大学动物科学技术学院，导师:李德法，pp.20-28)
[10]Ma Q Z，and Liu Q.Advance of Lectin Research.Guangdong Feed，15(5):23-25（马秋枝，刘强.2006.植物凝集泰的研究进展.广东饲料，15（5）:23-25)
[11]Muzilla Unklesbay M N，Helsel Z，Unklesbay K，and Ellersieck M.1989.Effect of particle size and heat on absorptive properties of soyhulls.Journal of Food Quality，12：305-318
[12]Pan H B，Qin G X，and Sun Z W.2005.Resarch Advances of Antinutrition on Soybean Lectin Antinutrition.Soybean Science，24 (3)：210-215（潘洪彬，秦贵信，孙泽威.2005.大豆凝集素抗营养的研究进展，大豆科学，24（3）：210-215
[13]Perez M D，Mills E N C，Lambert N，Johnson I T，and Morgan M R A.2000.The use of anti-soya globulin antisera in investigating soya digestion in vivo.Science of Food and Agriculture，80：513-521
[14]Rackis J J，Anderson R L，Sasame H A，Smith A K，and VanEtten C H.1961.Amino acids in soybean hulls and oil meal fractions.Journal of Agricultural Food Chemistry，9:409-412?
[15]Sharon N，and Lis H.2002.How proteins bind carbohydrate:lessons from legume lectins.Journal of Agricultural Food Chemistry，50：6586-6591
[16]Sissons J W.1982.Effects of soybean products on digestive processes in the gastrointestinal tract of preruminant calves.Proceedings of the Nutrition Society，41:53- 61?
[17]Yang Y P，Tong J M，Sa R N，and Zhang S Y.2006.Research advances of allergic factor one of the soybean antinutritional factors.China Animal Husbandry and Veterinary Medicine，33（11）：7-10(杨昱萍，佟建明，萨仁娜，章世元.2006.大豆抗营养因子—致敏因子的研究进展.中国畜牧兽医，33（11）：7-10

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备注/Memo

基金项目：国家自然科学基金重点项目（30430520）

作者简介：王涛（1984-），男，硕士研究生，研究方向饲料抗营养因子。E-mail: faithhill2002@163.com。
通讯作者(Corresponding author)：秦贵信，教授，博士生导师。

更新日期/Last Update: 2014-10-09